公开(公告)号：US12105122B2

公开(公告)日：2024-10-01

申请号：US18160764

申请日：2023-01-27

申请人： HOWARD HUGHES MEDICAL INSTITUTE

发明人： Eric R. Schreiter ,  Ahmed Abdelfattah

IPC分类号： G01R15/22 ,  C07K14/195 ,  C07K14/435 ,  G01N21/64 ,  G01R19/00

CPC分类号： G01R15/22 ,  G01R19/0084 ,  C07K14/195 ,  C07K14/43504 ,  C07K2319/60 ,  G01N21/6486

摘要： A voltage indicator includes a polypeptide sequence comprising a voltage-sensitive opsin domain and a capture protein domain arranged and disposed to capture a fluorescent dye ligand. When the fluorescent dye ligand is captured and the voltage indicator is bound to a cell membrane, an increase in voltage across the cell membrane causes an increase in fluorescent emission.

公开(公告)号：US11598792B2

公开(公告)日：2023-03-07

申请号：US16872188

申请日：2020-05-11

申请人： HOWARD HUGHES MEDICAL INSTITUTE

发明人： Eric R. Schreiter ,  Ahmed Abdelfattah

IPC分类号： G01R15/22 ,  G01R19/00 ,  C07K14/435 ,  G01N21/64

摘要： A voltage indicator includes a polypeptide sequence comprising a voltage-sensitive opsin domain and a capture protein domain arranged and disposed to capture a fluorescent dye ligand. When the fluorescent dye ligand is captured and the voltage indicator is bound to a cell membrane, an increase in voltage across the cell membrane causes an increase in fluorescent emission.

公开(公告)号：US11708397B2

公开(公告)日：2023-07-25

申请号：US16768153

申请日：2018-12-31

申请人： HOWARD HUGHES MEDICAL INSTITUTE

发明人： Eric R Schreiter ,  Luke D. Lavis ,  Claire Deo ,  Hersh Bhargava ,  Ahmed Abdelfattah

IPC分类号： C07K14/47 ,  G01N33/58 ,  G01N33/84 ,  G01N21/77 ,  A61K49/00

CPC分类号： C07K14/4728 ,  A61K49/0004 ,  A61K49/0041 ,  A61K49/0056 ,  G01N21/77 ,  G01N33/582 ,  G01N33/84 ,  C07K2319/20 ,  C07K2319/60 ,  G01N2021/7786 ,  G01N2333/4727

摘要： A chemigenetic calcium indicator and a method of measuring calcium are provided. The chemigenetic calcium indicator includes a calcium-binding protein domain attached to a ligand binding protein domain. The method of measuring calcium includes administering a chemigenetic calcium indicator to a subject and determining changes in fluorescence, the chemigenetic calcium indicator including a ligand binding protein domain having a calcium-binding protein domain and a dye-ligand conjugate attached thereto.

公开(公告)号：US11385236B2

公开(公告)日：2022-07-12

申请号：US16347765

申请日：2017-11-30

申请人： HOWARD HUGHES MEDICAL INSTITUTE

发明人： Eric R. Schreiter ,  Luke D. Lavis ,  Ahmed Abdelfattah

IPC分类号： G01N33/58 ,  C07K14/195 ,  C07K14/215 ,  C07K14/435 ,  C07K14/46 ,  C07K14/465 ,  C12N9/14 ,  G01N21/64 ,  G01N33/566

摘要： Provided herein are a voltage indicator and a method of measuring voltage. The voltage indicator includes a membrane-localized voltage-sensitive protein coupled to a capture protein. The method of measuring voltage includes administering a voltage indicator including a membrane-localized voltage-sensitive protein coupled to a capture protein, and determining changes in fluorescence of a small-molecule fluorescent dye captured by the capture protein.