公开(公告)号：US20150176070A1

公开(公告)日：2015-06-25

申请号：US14414235

申请日：2013-07-11

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Yuichiro Ota ,  Tomohiro Shoji ,  Michiru Fujioka ,  Takanobu Haga

IPC: C12Q1/68 ,  G01N21/03 ,  G01N15/14 ,  G01N21/64

CPC classification number: C12Q1/6869 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/0332 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/645 ,  G01N33/5436 ,  G01N2015/0038 ,  G01N2015/1006 ,  G01N2021/6482 ,  G01N2201/0231 ,  G01N2201/061

Abstract: In a biomaterial analysis, erroneous detection of a particle emitting fluorescence is prevented, and highly sensitive and highly accurate optical detection in biomaterial analysis is performed. A flow cell (104) for biomaterial analysis includes: a light-transmissive upper substrate (310); an antireflective lower substrate (313); and an inner layer section interposed between the upper substrate (310) and the lower substrate (313) and including a flow path (311) in which a particle (312) configured to emit fluorescence is provided. A biomaterial analysis device includes: a flow cell (104) for biomaterial analysis as described above; and an irradiation unit configured to irradiate excitation light; and an optical detection unit (106) configured to detect fluorescence emitted by the particle (312).

Abstract translation: 在生物材料分析中，防止发射荧光的颗粒的错误检测，并且进行生物材料分析中的高灵敏度和高精度的光学检测。 用于生物材料分析的流动池（104）包括：透光上基板（310）; 抗反射下基板（313）; 以及插入在上基板（310）和下基板（313）之间的内层部分，并且包括设置有发射荧光的颗粒（312）的流路（311）。 生物材料分析装置包括：如上所述的用于生物材料分析的流动池（104）; 以及被配置为照射激发光的照射单元; 以及被配置为检测由所述粒子（312）发射的荧光的光学检测单元（106）。

公开(公告)号：US10451553B2

公开(公告)日：2019-10-22

申请号：US15129287

申请日：2015-03-18

Applicant: Hitachi High-Technologies Corporation

Inventor： Motohiro Yamazaki ,  Yuichiro Ota ,  Satoshi Takahashi ,  Yoshitaka Kodama

IPC: G01N21/64 ,  G01J3/44 ,  G01N27/447 ,  G01J3/02

Abstract: During analysis of samples of unknown concentration, situations frequently occur in which the dynamic range is insufficient, necessitating reanalysis. Accordingly, a fluorescence spectrometer which splits a single object image into multiple images having different fluorescent intensity by means of image splitting elements, and simultaneously detects the plurality of images obtained thereby in different regions within the same detection plane, is proposed.

公开(公告)号：US10711294B2

公开(公告)日：2020-07-14

申请号：US15533087

申请日：2014-12-26

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Yuichiro Ota ,  Tomohiro Shoji ,  Toru Yokoyama ,  Masatoshi Narahara

IPC: C12Q1/68 ,  G01B11/00 ,  C12Q1/6837 ,  G01N21/05 ,  G01N21/64 ,  G01N35/00

Abstract: The substrate 100 for use in the analysis of a nucleic acid according to the present invention has multiple analysis areas 12 which are partitioned on a substrate 10, and enables the measurement of the analysis areas 12 while interchanging the analysis areas 12 in turn, said substrate 100 being characterized in that each of the analysis areas 12 consists of an adsorption part 13 onto which a DNA fragment or a carrier having the DNA fragment carried thereon can be adsorbed and a non-adsorption part 14 which is a part outside of the adsorption part 13, and the non-adsorption part 14 has, formed on at least a part thereof, a marker part 15 that has a specified shape and helps to identify the positions of the analysis areas 12.