公开(公告)号：US10711294B2

公开(公告)日：2020-07-14

申请号：US15533087

申请日：2014-12-26

Applicant: HITACHI HIGH-TECHNOLOGIES CORPORATION

Inventor： Yuichiro Ota ,  Tomohiro Shoji ,  Toru Yokoyama ,  Masatoshi Narahara

IPC: C12Q1/68 ,  G01B11/00 ,  C12Q1/6837 ,  G01N21/05 ,  G01N21/64 ,  G01N35/00

Abstract: The substrate 100 for use in the analysis of a nucleic acid according to the present invention has multiple analysis areas 12 which are partitioned on a substrate 10, and enables the measurement of the analysis areas 12 while interchanging the analysis areas 12 in turn, said substrate 100 being characterized in that each of the analysis areas 12 consists of an adsorption part 13 onto which a DNA fragment or a carrier having the DNA fragment carried thereon can be adsorbed and a non-adsorption part 14 which is a part outside of the adsorption part 13, and the non-adsorption part 14 has, formed on at least a part thereof, a marker part 15 that has a specified shape and helps to identify the positions of the analysis areas 12.

公开(公告)号：US10041884B2

公开(公告)日：2018-08-07

申请号：US14890246

申请日：2014-05-09

Applicant: Hitachi High-Technologies Corporation

Inventor： Michiru Fujioka ,  Motohiro Yamazaki ,  Toru Yokoyama

IPC: G01N21/64 ,  G06F19/24 ,  C12Q1/68

Abstract: Provided is a nucleic acid analyzer, which does not require manual processes by a highly trained operator such as a researcher and is easy to use, small-sized, capable of accepting multiple samples, and performs speedy analysis, and a nucleic acid analysis method using the analyzer. The analyzer and method perform detection in a plurality of exposure times, provide a program for determining a threshold for signal detection, and determine whether a faint signal peak is a false signal peak.

公开(公告)号：US09605300B2

公开(公告)日：2017-03-28

申请号：US14652922

申请日：2013-12-04

Applicant: Hitachi High-Technologies Corporation

Inventor： Toru Yokoyama ,  Motohiro Yamazaki

IPC: G01J3/30 ,  C12Q1/68 ,  G06F19/18 ,  G01N27/447 ,  G01N21/64

CPC classification number: C12Q1/68 ,  C12Q1/6827 ,  G01N21/6428 ,  G01N21/6456 ,  G01N27/447 ,  G01N27/44726 ,  G01N2021/6417 ,  G01N2021/6439 ,  G01N2021/6441 ,  G06F19/18 ,  C12Q2565/629 ,  C12Q2565/634

Abstract: A technique for performing spectral calibration simultaneously with electrophoresis of an actual sample to be analyzed, without performing electrophoresis using a special matrix standard which is time-consuming and costly, is provided. The device for genotypic analysis is characterized by obtaining reference fluorescence spectra using a size standard and an allelic ladder, which provide information concerning known DNA fragments used for electrophoresis of an actual sample, and is characterized by performing spectral calibration for a capillary in which the allelic ladder is not used by detecting a shift amount of the fluorescence spectra of the size standard and shifting the reference fluorescence spectra using the shift amount to determine fluorescence spectra.