公开(公告)号：US10329525B2

公开(公告)日：2019-06-25

申请号：US15323977

申请日：2014-07-23

Applicant: Hitachi, Ltd.

Inventor： Masaharu Kiyama ,  Guangbin Zhou ,  Takayuki Nozaki ,  Ryota Nakajima ,  Daisuke Suzuki ,  Yumiko Igarashi ,  Shizu Takeda ,  Taku Nakamura ,  Masakazu Sugaya ,  Koichi Terada

IPC: C12M1/00 ,  C12M1/34 ,  C12M1/36

Abstract: There is the problem that a liquid culture medium fed by a pump has a high frequency of contact with a gas during movement in a tube, and the pH value of the liquid culture medium before and after feeding changes easily. In a liquid feeder and a cell culture apparatus provided therewith, a feed pipe 7 is connected with a gas bag 14, the liquid to be fed into the feed pipe 7 is fed so as to be sandwiched by a gas for suppressing the qualitative changes of the liquid. Furthermore, the gas bag is connected with a container 8 for holding a liquid, and holds the gas for suppressing qualitative changes of the liquid in a gas phase of the container.

公开(公告)号：US10087410B2

公开(公告)日：2018-10-02

申请号：US14770627

申请日：2013-03-25

Applicant: Hitachi, Ltd.

Inventor： Takayuki Nozaki ,  Guangbin Zhou ,  Masaharu Kiyama ,  Taku Nakamura ,  Shizu Takeda ,  Ryota Nakajima ,  Masakazu Sugaya ,  Koichi Terada

IPC: C12M1/34 ,  C12M1/00 ,  C12M1/36 ,  C12M3/00

Abstract: In culture vessels for producing biological samples, it is possible to prevent decrease in a temperature at the time of taking out the culture vessels from an automatic culture apparatus and during delivery of the culture vessels which have been taken out from the automatic culture apparatus. This invention includes a culture vessel 201 holding a biological sample therein, a heat storage portion 701 holding the culture vessel, and a heat insulation portion 601 surrounding the heat storage portion. The whole circumference or a part of 609, 610, and 611 of the heat insulation portion 601 is removable depending on an arrangement environment.

公开(公告)号：US10087409B2

公开(公告)日：2018-10-02

申请号：US14426457

申请日：2012-09-26

Applicant: Hitachi, Ltd.

Inventor： Masaharu Kiyama ,  Guangbin Zhou ,  Ryota Nakajima ,  Takayuki Nozaki ,  Naoko Senda ,  Shizu Matsuoka

IPC: C12M1/22 ,  C12M1/00 ,  C12M1/42 ,  C12M3/00 ,  C12M1/12 ,  C12M1/36

Abstract: Provided is a cell culturing vessel for holding and culturing cells. It has a first vessel that stores a culture medium and cells or only a culture medium, a second vessel that is placed above the first vessel and stores a culture medium and cells or only a culture medium, a main vessel that holds the first vessel and houses the second vessel, and a lid member that engages with the main vessel. The main vessel has a pressing member that fixes and holds the first vessel in the main vessel and the second vessel is eccentrically held in the first vessel by the pressing member.

公开(公告)号：US20150232798A1

公开(公告)日：2015-08-20

申请号：US14425331

申请日：2012-09-05

Applicant: Hitachi,, Ltd.

Inventor： Guangbin Zhou ,  Takayuki Nozaki ,  Shizu Matsuoka ,  Ryota Nakajima ,  Masaharu Kiyama ,  Kenichi Minami

IPC: C12M1/00 ,  G05D7/00 ,  F16K7/06

CPC classification number: C12M29/00 ,  A61M39/281 ,  A61M39/284 ,  A61M39/285 ,  C12M29/06 ,  F16K7/063 ,  G05D7/00 ,  Y10T137/87

Abstract: The purpose of the present invention is to provide a valve mechanism which features a simple structure adapted to collectively control the supply of gas or culture medium to a plurality of cultivation containers for simultaneous cultivation and to enhance the reliability of the valve mechanism. The valve mechanism of the invention is a valve mechanism for opening and closing passages for liquid or gas. The valve mechanism includes: clip members adapted to close a plurality of tube passages, respectively; a multiple cam member for opening the clip members in sequence by making contact with the clip members; and a rotational mechanism for rotating the multiple cam member. The clip member includes: a spring for closing a clip thereof; and a clip contact portion for a cam contact portion of the multiple cam member to make contact therewith.

Abstract translation: 本发明的目的是提供一种具有简单结构的阀机构，其适于共同控制气体或培养基供应到多个栽培容器以用于同时培养并提高阀机构的可靠性。 本发明的阀机构是用于打开和关闭用于液体或气体的通道的阀机构。 阀机构包括：适于分别关闭多个管道的夹子构件; 多个凸轮构件，用于通过与夹子构件接触来依次打开夹子构件; 以及用于旋转所述多个凸轮构件的旋转机构。 夹子构件包括：用于封闭其夹子的弹簧; 以及用于所述多个凸轮构件的凸轮接触部分以与其接触的夹子接触部分。

公开(公告)号：US10138450B2

公开(公告)日：2018-11-27

申请号：US14768770

申请日：2013-03-15

Applicant: Hitachi, Ltd.

Inventor： Takayuki Nozaki ,  Guangbin Zhou ,  Masaharu Kiyama ,  Ryota Nakajima ,  Shizu Matsuoka ,  Taku Nakamura ,  Masakazu Sugaya ,  Koichi Terada

IPC: C12M1/00 ,  B01L1/00 ,  C12M3/00 ,  C12M1/34

Abstract: An automatic culture device which employs closed-system culture vessels and in which, when a passage part (2) and a rotational valve mechanism (3) are configured, the multiple culture vessels in a culture vessel part (1) can be rendered equal in liquid-feeding conditions by, for example, regulating the length of each passage. As a result, the evenness in quality of cells incubated in the multiple culture vessels is rendered possible. Furthermore, by providing a mechanism which enables a user to normally set passages in the device, the evenness in quality of the cells is rendered possible likewise.

公开(公告)号：US20160244713A1

公开(公告)日：2016-08-25

申请号：US15029762

申请日：2013-10-24

Applicant: HITACHI, LTD.

Inventor： Taku Nakamura ,  Takayuki Nozaki ,  Koichi Terada ,  Masaharu Kiyama ,  Guangbin Zhou ,  Masakazu Sugaya ,  Shizu Matsuoka

IPC: C12M1/36 ,  C12M1/34 ,  C12M1/00

CPC classification number: C12M41/48 ,  C12M23/50 ,  C12M23/58 ,  C12M29/00 ,  C12M41/00 ,  C12M41/34 ,  C12M41/40

Abstract: Provided is an automated culture apparatus in which the exchange of a culture medium or the exchange of a gas in a culture vessel that is taken from the device is not needed. The device is equipped with multiple culture vessels (500 to 530), flow channels respectively connected to the multiple culture vessels, a vessel selection unit (300) for controlling a liquid or a gas that is to be sent to the culture vessels through the flow channels, and a control unit (400) for controlling the vessel selection unit, wherein the control unit (400) detects the disconnection of some of the flow channels which correspond to some of the culture vessels among the multiple culture vessels and alters the manner of the control of the sending of the liquid or the gas.

Abstract translation: 提供一种自动培养装置，其中不需要从装置取出培养基的交换或培养容器中的气体交换。 该装置配备有多个培养容器（500至530），分别连接到多个培养容器的流动通道，用于控制待通过流动送至培养容器的液体或气体的容器选择单元（300） 通道和用于控制血管选择单元的控制单元（400），其中控制单元（400）检测与多个培养容器中的一些培养容器相对应的一些流动通道的断开，并改变 控制液体或气体的发送。

公开(公告)号：US11441114B2

公开(公告)日：2022-09-13

申请号：US16316908

申请日：2017-02-24

Applicant: Hitachi, Ltd.

Inventor： Masaharu Kiyama ,  Guangbin Zhou ,  Daisuke Suzuki ,  Midori Kato ,  Hikaru Saito

IPC: C12M1/12 ,  C12M1/00 ,  C12M1/34 ,  C12M1/36

Abstract: A liquid feed device that feeds a liquid medium and a cell suspension to target containers by respectively suited liquid feed methods is provided. A pump 6 is connected between a first liquid bottle 12 containing a liquid medium and a second liquid bottle 2 containing a cell suspension, and a receptacle 8 to be used as a culture container is connected downstream of a second liquid bottle 12. The liquid medium in the first liquid bottle is sent to the receptacle through the pump 6 and a branch point 20 by opening a first supply valve 17, and the cell suspension in the second liquid bottle is sent to the receptacle by opening a first gas introducing valve 10, a second gas introducing valve 15, and a second supply valve 19 and transferring, by pressure, a gas supplied from the first gas introducing valve 10 to the second liquid bottle through the pump 6 and a branch point 16.

公开(公告)号：US10184100B2

公开(公告)日：2019-01-22

申请号：US14894033

申请日：2013-08-23

Applicant: Hitachi, Ltd.

Inventor： Masaharu Kiyama ,  Guangbin Zhou ,  Takayuki Nozaki ,  Shizu Matsuoka ,  Masakazu Sugaya ,  Taku Nakamura ,  Koichi Terada

IPC: C12M3/00 ,  C12M1/34 ,  C12M1/00 ,  C12M1/26

Abstract: The liquid feeder includes a liquid feeding pipe having a liquid introduce port and a liquid discharge port, a housing container which holds liquid to be introduced from the liquid introduce port, a liquid feed mechanism which feeds the liquid in the liquid feeding pipe to the liquid discharge port, a gas introduce unit which introduces gas into the liquid feeding pipe, and a liquid level detection unit which detects an advancing liquid level of the liquid to be fed in the liquid feeding pipe. The gas introduce unit is connected to a branch part provided in the liquid feeding pipe on the upstream side of the liquid feed mechanism. The liquid level detection unit is provided on the downstream side of the branch part.

公开(公告)号：US20160244711A1

公开(公告)日：2016-08-25

申请号：US15027485

申请日：2013-10-15

Applicant: HITACHI, LTD.

Inventor： Masaharu Kiyama ,  Guangbin Zhou ,  Takayuki Nozaki

IPC: C12M1/34 ,  C12M1/00

CPC classification number: C12M41/34 ,  C12M29/26

Abstract: Maintaining pressure inside a culturing vessel is difficult when minimizing the size of a liquid supply path that supplies liquid and gas to a cell culturing vessel that performs two-level culturing (co-culturing). This cell culturing device includes: culturing vessels; a flow path having at least first supply ports capable of supplying liquid or gas to the culturing vessels and first discharge ports that discharge the gas from the culturing vessels, said flow path discharging gas from the first discharge ports to the atmosphere; and a filter in the flow path. A trap bottle is provided between the first discharge ports and the filter upon the flow path to collect moisture from the discharged gas. Thus, a liquid phase and a gas phase are separated in the trap bottle; moisture is prevented from reaching the filter; filter clogging is prevented; and the internal pressure of the culturing vessels can be maintained.

Abstract translation: 在将液体和气体供给到进行两级培养（共培养）的细胞培养容器的液体供给路径的尺寸最小化的情况下，难以维持培养容器内的压力。 该细胞培养装置包括：培养容器; 具有能够向培养容器供给液体或气体的至少第一供给口和从培养容器排出气体的第一排出口的流路，所述流路从第一排出口向大气排出气体; 和流路中的过滤器。 在流路上的第一排出口和过滤器之间设置有捕集瓶，以从排出的气体中收集水分。 因此，在捕集瓶中分离液相和气相; 防止水分到达过滤器; 防止过滤器堵塞; 并且能够维持培养容器的内部压力。

公开(公告)号：US10947490B2

公开(公告)日：2021-03-16

申请号：US15896947

申请日：2018-02-14

Applicant: HITACHI, LTD.

Inventor： Hikaru Saito ,  Masaharu Kiyama ,  Midori Kato ,  Kunio Ohyama ,  Guangbin Zhou ,  Hiroko Hanzawa ,  Shizu Takeda

IPC: C12M1/00 ,  C12M1/36 ,  C12M1/34 ,  C12M1/12 ,  C12M3/00

Abstract: A liquid delivery device includes a first and second container for a first and second liquid; a third container for receiving a first and second liquid; a fourth container into which a first and second liquid are discharged; a fifth container for a third liquid; a first liquid delivery tube for delivering a first liquid; a second liquid delivery tube for delivering a second liquid; a third liquid delivery tube connected to the first and second liquid delivery tube; a first liquid delivery pump on the third liquid delivery tube; a fourth liquid delivery tube for discharging a first liquid contained in the third container and delivering a third liquid to the third container; a fifth liquid delivery tube connected to the fourth liquid delivery tube; a sixth liquid delivery tube connected to the fourth liquid delivery tube; and a second liquid delivery pump on the fourth liquid delivery tube.