Abstract:
In an image acquisition device, an optical path length difference in a second light figure can be formed by arrangement of an optical path difference generating member, without need for splitting light in a second optical path for focus control. Therefore, it reduces the quantity of light into the second optical path necessary for acquisition of information of focal position while ensuring the quantity of light enough for execution of imaging by a first imaging device. Furthermore, in this image acquisition device, a light reduction portion is provided between a first face and a second face of the optical path difference generating member. This light reduction portion can narrow a light superimposed region on the imaging surface of the second imaging device, which allows control of the focal position to a sample to be accurately carried out.