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公开(公告)号：US20050196327A1
公开(公告)日：2005-09-08
申请号：US11080071
申请日：2005-03-15
Applicant: Gregory Mathus , George Lyman
Inventor： Gregory Mathus , George Lyman
CPC classification number: B01L3/50853
Abstract: A method of sealing the open mouths of an array of test tubes comprises providing a plurality of plugs with upwardly facing recesses, and a flexible carrier sheet having the same array of mutually spaced downwardly projecting pegs inserted into and frictionally retained within the recesses of respective ones of the plugs. Sealing is effected by aligning the plugs with the open mouths of the test tubes, pressing the carrier sheet towards the test tubes to seat the plugs in the open tube mouths, and peeling the carrier sheet away from the test tubes to extract the pegs from the plug recesses while allowing the plugs to remain seated in the tube mouths.
Abstract translation: 密封试管阵列的开口的方法包括提供多个具有向上的凹部的塞子，以及柔性载体片，其具有插入并摩擦地保持在相应的凹槽内的相互间隔开的向下突出的钉的相同阵列 的插头。 密封是通过将塞子与试管的开口对准来实现的，将载体片朝向试管按压以将塞子固定在开口管口中，并将载体片材从试管上剥离，以将塞子从 插头凹槽，同时允许插头保持在管口中。
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公开(公告)号：US06890488B2
公开(公告)日：2005-05-10
申请号：US10165032
申请日：2002-06-07
Applicant: Gregory Mathus , George Lyman
Inventor： Gregory Mathus , George Lyman
CPC classification number: B01L3/50853
Abstract: A sealing mat for sealing the open mouths of an array of test tubes. The mat includes a flexible carrier sheet having an array of mutually spaced pegs projecting downwardly therefrom and a plurality of plugs. The plugs are externally configured and dimensioned to be inserted in and to seal the open mouths of the test tubes. Each of the plugs has an upwardly facing recess internally configured and dimensioned to receive and coact in frictional engagement with a respective one of the pegs to thereby releasably secure the plugs to the carrier sheet. The carrier sheet is separable from the thus inserted plugs by flexure to extract the pegs from the recesses.
Abstract translation: 用于密封试管阵列的开口的密封垫。 垫包括柔性载体片，其具有从其向下突出的相互间隔开的钉的阵列和多个塞子。 插头外部配置和尺寸设计成插入并密封试管的开放口。 每个插头具有内部构造的尺寸设计为上部的凹槽，其尺寸被设计成接收并与其相应的一个棘爪摩擦接合，从而将插头可释放地固定到载体片上。 载体片通过挠曲与这样插入的插塞分离，以从凹部提取钉。
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公开(公告)号：US5139951A
公开(公告)日：1992-08-18
申请号：US595305
申请日：1990-10-10
Applicant: David Butz , George Lyman , David Root , Gregory Mathus
Inventor： David Butz , George Lyman , David Root , Gregory Mathus
IPC: C12M3/06
Abstract: A tissue or cell growth device is described for placement within a well of a cluster dish. The device has a cell or tissue retention element detachably attached to a hanger. The hanger preferably has openings allowing access to the well without removal of the member and is capable of positioning the cell or tissue retention element a preselected distance relative to the hanger. Also described are various embodiments of the device having differently shaped hangers and the combination of the device and a cluster dish.
Abstract translation: 描述了组织或细胞生长装置用于放置在簇盘的孔内。 该装置具有可拆卸地附接到衣架的细胞或组织保持元件。 衣架优选地具有允许进入井的开口，而不移除构件，并且能够将电池或组织保持元件相对于衣架定位成预选的距离。 还描述了具有不同形状的悬挂器以及装置和簇盘的组合的装置的各种实施例。
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公开(公告)号：US5026649A
公开(公告)日：1991-06-25
申请号：US841562
申请日：1986-03-20
Applicant: George Lyman , Gregory Mathus , David Root
Inventor： George Lyman , Gregory Mathus , David Root
Abstract: Apparatus for growing tissue cultures in vitro, which permits a concentration gradient of nutrients to develop through a permeable membrane to which a sample of tissue is attached. The permeable membrane is attached to the bottom end of a tubular support that in turn hangs by a flange connected to its upper end on the top of a well containing the nutrients. Typically, the well is part of a tissue culture cluster dish. The flange of the support positions the support and membrane centrally in the well so as to avoid capillary action in the space between the well and support. The configuration of the support and its cooperation with the lid of the cluster dish also prevents the support and membrane from floating in the nutrient solution in the well. Openings in the support provide access for a pipette to add and withdraw fluid from the space between the well and membrane support and from the space below the membrane.
Abstract translation: 用于在体外生长组织培养物的装置，其允许营养物的浓度梯度通过附着有组织样品的渗透膜发育。 可渗透膜附接到管状支撑件的底端，管状支撑件的底端又通过连接到容纳营养物质的孔的顶部上方的凸缘而悬挂。 通常，孔是组织培养簇盘的一部分。 支撑件的凸缘将支撑件和膜中心定位在井中，以避免在井和支撑件之间的空间中的毛细作用。 支架的配置及其与簇盘盖的配合也可防止支撑和膜浮在井中的营养液中。 支架中的开口提供移液管的通道，以从孔和膜支撑之间的空间以及膜下方的空间添加和抽出流体。
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公开(公告)号：US4927764A
公开(公告)日：1990-05-22
申请号：US122052
申请日：1987-11-17
Applicant: George Lyman , Gregory Mathus
Inventor： George Lyman , Gregory Mathus
Abstract: A tissue culture flask having a flat bottom wall with optical properties on the inner surface of which cells are grown. A top wall opposite the bottom wall has a large opening therethrough which provides access to the growing surface for the removal of cells growing on the surface. The opening is closed by a flexible transparent film which is sealed to the top wall and which may be peeled off the top wall to provide access to the flask interior.
Abstract translation: 一种组织培养瓶，其具有在其内表面上生长细胞的具有光学性质的平坦底壁。 与底壁相对的顶壁具有穿过其的大开口，其提供进入生长表面以去除在表面上生长的细胞。 开口由柔性透明膜封闭，该透明膜密封到顶壁，并且可以从顶壁剥离以提供通向烧瓶内部的通路。
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Patent Agency Ranking
公开(公告)号：US4690757A
公开(公告)日：1987-09-01
申请号：US842494
申请日：1986-03-21
Applicant: Gregory Mathus , George Lyman , Kevin Looney
Inventor： Gregory Mathus , George Lyman , Kevin Looney
CPC classification number: B01D46/543 , B01D29/012 , B01D46/0012 , B01D61/18 , B01D63/08 , B01D2277/20
Abstract: A small volume laboratory filter having a thin rectangular body and coaxial inlet and outlet sleeves connected to opposite ends thereof. A very thin filter cavity is formed in the body and lies in a plane inclined slightly to the common axis of the inlet and outlet sleeves. The cavity is divided by a filter membrane into extremely thin chambers, one communicating with the inlet sleeve and the other with the outlet sleeve. A rigid package for the filter is sealed across one end by a flexible cover which when stripped from the package exposes the inlet sleeve so that it may be readily engaged by a syringe nipple or other fluid source without the user having to touch the filter. When the inlet sleeve is engaged, the filter may be withdrawn from the package.
Abstract translation: 具有薄的矩形体的小体积实验室过滤器和连接到其相对端的同轴入口和出口套筒。 在体内形成非常薄的过滤腔，并且位于与入口和出口套筒的公共轴线稍微倾斜的平面内。 空腔被过滤膜分成非常薄的室，一个与入口套管相通，另一个与出口套管连通。 用于过滤器的刚性包装通过柔性盖密封在一端上，当从包装件剥离时，柔性罩暴露入口套筒，使得其可以容易地通过注射器管接头或其它流体源接合，而用户不必接触过滤器。 当入口套筒接合时，过滤器可以从包装件中取出。
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公开(公告)号：US07306767B2
公开(公告)日：2007-12-11
申请号：US11080071
申请日：2005-03-15
Applicant: Gregory Mathus , George Lyman
Inventor： Gregory Mathus , George Lyman
IPC: B01L3/00
CPC classification number: B01L3/50853
Abstract: A method of sealing the open mouths of an array of test tubes comprises providing a plurality of plugs with upwardly facing recesses, and a flexible carrier sheet having the same array of mutually spaced downwardly projecting pegs inserted into and frictionally retained within the recesses of respective ones of the plugs. Sealing is effected by aligning the plugs with the open mouths of the test tubes, pressing the carrier sheet towards the test tubes to seat the plugs in the open tube mouths, and peeling the carrier sheet away from the test tubes to extract the pegs from the plug recesses while allowing the plugs to remain seated in the tube mouths.
Abstract translation: 密封试管阵列的开口的方法包括提供多个具有向上的凹部的塞子，以及柔性载体片，其具有插入并摩擦地保持在相应的凹槽内的相互间隔开的向下突出的钉的相同阵列 的插头。 密封是通过将塞子与试管的开口对准来实现的，将载体片朝向试管按压以将塞子固定在开口管口中，并将载体片材从试管上剥离，以将塞子从 插头凹槽，同时允许插头保持在管口中。
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公开(公告)号：USD245300S
公开(公告)日：1977-08-09
申请号：US672780
申请日：1976-04-01
Applicant: Gregory Mathus , George Lyman , David Roche
Designer： Gregory Mathus , George Lyman , David Roche
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9.发明授权Culture device and method of use having a detachable cell or tissue growth surface 失效具有可分离细胞或组织生长表面的培养装置和使用方法
公开(公告)号：US5272083A
公开(公告)日：1993-12-21
申请号：US705767
申请日：1991-05-28
Applicant: David Butz , George Lyman , David Root , Gregory Mathus
Inventor： David Butz , George Lyman , David Root , Gregory Mathus
Abstract: A tissue or cell growth device is described for placement within a well of a cluster dish. The device has a cell or tissue retention element detachably attached to a hanger. The hanger preferably has openings allowing access to the well without removal of the member and is capable of positioning the cell or tissue retention element a preselected distance relative to the hanger. Also described are various embodiments of the device having differently shaped hangers and the combination of the device and a cluster dish.
Abstract translation: 描述了组织或细胞生长装置用于放置在簇盘的孔内。 该装置具有可拆卸地附接到衣架的细胞或组织保持元件。 衣架优选地具有允许进入井的开口，而不移除构件，并且能够将电池或组织保持元件相对于衣架定位成预选的距离。 还描述了具有不同形状的悬挂器以及装置和簇盘的组合的装置的各种实施例。
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公开(公告)号：US5466602A
公开(公告)日：1995-11-14
申请号：US180695
申请日：1994-01-13
Applicant: George Lyman , Gregory Mathus , David Root
Inventor： George Lyman , Gregory Mathus , David Root
Abstract: The present invention relates to an apparatus for growing tissue culture in vitro. The apparatus features a housing which fits into a well containing media. A bottom membrane surface is maintained at a depth within the well, and the housing centered in the well by a flange and rim which cooperate with the well.
Abstract translation: 本发明涉及体外培养组织培养的装置。 该装置具有适合于容纳介质的容器的壳体。 底部膜表面保持在井内的深度，并且壳体通过与井配合的凸缘和边缘在井中居中。
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