公开(公告)号：US20080311631A1

公开(公告)日：2008-12-18

申请号：US11628459

申请日：2005-06-02

Applicant: Marcel Gerhardus Wubbolts ,  Roelof Ary Lans Bovenberg ,  Georg Sprenger ,  Johannes Josef Bongaerts ,  Stefan Kozak ,  Michael Muller ,  Volker Lorbach

Inventor： Marcel Gerhardus Wubbolts ,  Roelof Ary Lans Bovenberg ,  Georg Sprenger ,  Johannes Josef Bongaerts ,  Stefan Kozak ,  Michael Muller ,  Volker Lorbach

IPC: C12P13/22 ,  C12P13/00 ,  C12N15/63 ,  C12N1/20 ,  C07C61/22

CPC classification number: C12P13/04

Abstract: The invention relates to a process for the biosynthetic production of 4-amino-4-deoxychorismate (ADC) performed fermentatively in vivo with a 4-amino-4-deoxychorismate synthase, preferably a PabAB bipartite protein (which may be a fusion protein), at an increased level of activity, thereby obtaining a broth comprising ADC and 4-amino-4-deoxyprephenate (ADP), that are recovered. The invention also relates to a further process of converting the ADP into p-aminophenylalanine. The invention, moreover relates to biosynthetic production of [3R,4R]-4-amino-3-hydroxycyclohexa-1,5-diene-1-carboxylic acid (3,4-CHA), by concerted action of such 4-amino-4-deoxychorismate synthase and of an enzyme capable of converting isochorismate into [5S,6S]-5,6 dihydroxycyclohexa-1,3-diene-1-carboxylic acid (2,3-CHD), preferably a phenazine biosynthesis protein PhzD, including recovery of 3,4-CHA. The invention also relates to expression vectors and host cells for use in any of such processes. The invention further relates to the use of 3,4-CHA as a catalytically active product, in particular as a chiral catalyst. And the invention finally relates to synthesis of oseltamivir phosphate from 3,4-CHA.

Abstract translation: 本发明涉及用4-氨基-4-脱氧激酶合酶，优选PabAB二分体蛋白（可以是融合蛋白）在体内发酵生物合成生产4-氨基-4-脱氧胸苷酸（ADC）的方法。 在增加的活性水平上，从而获得包含ADC和4-氨基-4-脱氧降钙素（ADP）的肉汤，其被回收。 本发明还涉及将ADP转化为对氨基苯丙氨酸的另一种方法。 本发明还涉及通过这种4-氨基-3-羟基环己基-1,5-二烯-1-羧酸（3,4-CHA）的共同作用生产合成[3R，4R] -4-氨基-3-羟基环己-1,5-二烯-1-羧酸（3,4-CHA） 能够将等分子酸转化为[5S，6S] -5,6二羟基环己-1,3-二烯-1-羧酸（2,3-CHD），优选吩嗪生物合成蛋白PhzD的酶的酶，包括4-脱氧胸苷酸合酶 回收3,4-CHA。 本发明还涉及用于任何这些方法的表达载体和宿主细胞。 本发明还涉及3,4-CHA作为催化活性产物，特别是作为手性催化剂的用途。 本发明最终涉及从3,4-CHA合成磷酸奥司他韦。

公开(公告)号：US06316232B1

公开(公告)日：2001-11-13

申请号：US09298843

申请日：1999-04-26

Applicant: Georg Sprenger ,  Ruth Siewe ,  Hermann Sahm ,  Martin Karutz ,  Theodorus Sonke

Inventor： Georg Sprenger ,  Ruth Siewe ,  Hermann Sahm ,  Martin Karutz ,  Theodorus Sonke

IPC: C12P1304

CPC classification number: C12N9/1205 ,  C07K14/195 ,  C12N9/1022 ,  C12P1/00 ,  C12P13/22 ,  C12P19/02

Abstract: The invention makes available, by means of an increased provision of intracellular metabolic intermediates, in particular of erythrose 4-phosphate, alternative processes for the microbial preparation of substances, in particular of aromatic amino acids such as L-phenylalanine, in which processes the activity of a transaldolase is increased in a microorganism producing these substances. In preferred embodiments of the invention, the activity of a transketolase or the activity of a transport protein for the PEP-independent uptake of a sugar and/or the activity of a kinase which phosporylates the relevant sugar are/is additionally increased. The invention also relates to gene structures, and to transformed cells carrying these gene structures, which make it possible to implement these processes in a particularly successful manner.

Abstract translation: 本发明通过增加细胞内代谢中间体，尤其是赤藓糖-4-磷酸的提供，可用于微生物制备物质，特别是芳族氨基酸如L-苯丙氨酸的替代方法，其中过程活性 的转醛醇酶在产生这些物质的微生物中增加。 在本发明的优选实施方案中，另外增加转酮醇酶的活性或转运蛋白的活性，用于PEP独立摄取糖和/或磷酸化相关糖的激酶的活性。 本发明还涉及基因结构以及携带这些基因结构的转化细胞，这使得可以以特别成功的方式实施这些过程。

公开(公告)号：US20050227333A1

公开(公告)日：2005-10-13

申请号：US10497587

申请日：2002-12-05

Applicant: Marc Gerigk ,  Leon Raeven ,  Georg Sprenger ,  Ralf Takors ,  Christian Wandrey

Inventor： Marc Gerigk ,  Leon Raeven ,  Georg Sprenger ,  Ralf Takors ,  Christian Wandrey

IPC: C12M1/36 ,  C12P7/22 ,  C12P13/22 ,  C12Q3/00

CPC classification number: C12P7/22 ,  C12P13/22

Abstract: The invention relates to a process for the production of an aromatic amino acid metabolite or derivative thereof by aerobic fermentation of Escherichia coli, which fermentation comprises a growth and a production phase and in which fermentation glucose and L-tyrosine are controlled, wherein during at least part of the production phase, the glucose concentration in the fermentation medium is controlled within the range of 1-20 g/L and the L-tyrosine concentration in the fermentation medium is controlled below 36 mg/L.

Abstract translation: 本发明涉及通过大肠杆菌的好氧发酵生产芳香族氨基酸代谢物或其衍生物的方法，该发酵包括生长和生产阶段，其中控制发酵葡萄糖和L-酪氨酸，其中至少 部分生产阶段，发酵培养基中的葡萄糖浓度控制在1-20g / L的范围内，发酵培养基中的L-酪氨酸浓度控制在36mg / L以下。

公开(公告)号：US4802059A

公开(公告)日：1989-01-31

申请号：US188186

申请日：1988-04-28

Applicant: Gunter Prietzel ,  Georg Sprenger ,  Reinhard Kugler

Inventor： Gunter Prietzel ,  Georg Sprenger ,  Reinhard Kugler

IPC: H01H71/08 ,  H02B1/20

CPC classification number: H01H71/08

Abstract: In a low-voltage circuit breaker, terminal points for connecting contact arrangements to stationary bus bars are provided. Between the terminal points and the stationary bus bars, connecting bars are arranged which are braced relative to the housing of the circuit breaker by support bodies against current forces. The support bodies are parts comprising relatively thin insulating material with cutouts for the connecting bars. Detent projections allow the support bodies to snap into corresponding recesses in the lateral housing walls of the circuit breaker.

Abstract translation: 在低压断路器中，提供用于将接触装置连接到固定母线的端点。 在终点和固定母线之间，连接杆布置成相对于断路器的壳体通过支撑体抵抗电流力而被支撑。 支撑体是包括相对薄的绝缘材料的部件，具有用于连接杆的切口。 止动突起允许支撑体卡入到断路器的侧向壳体壁中的相应凹部中。

公开(公告)号：US09091327B2

公开(公告)日：2015-07-28

申请号：US13473147

申请日：2012-05-16

Applicant: Georg Sprenger

Inventor： Georg Sprenger

IPC: F03D11/00 ,  F16H1/28 ,  F03D11/02 ,  F16H1/46

CPC classification number: F16H1/28 ,  F03D15/00 ,  F03D15/10 ,  F03D80/70 ,  F05B2240/61 ,  F05B2260/40311 ,  F16D1/033 ,  F16H1/46 ,  H02K5/225 ,  Y02E10/722

Abstract: A drive system for a wind turbine includes at least one planetary gear stage, connectable to a rotor shaft of the wind turbine and including a sun gear engaging with multiple planetary gears, a driven hollow shaft connected to the sun gear, a pitch tube having a generator-side end with a centering shoulder, and a face side provided with threaded holes, the pitch tube being arranged concentrically to the annulus gear and extending axially over the entirety of the planetary stage, an adapter element having an opening for receiving a generator add-on component, the adapter element being received on and radially surrounding the generator side end and fixed in position radially and axially in one direction by the centering shoulder, and screw-type connection fittings extending axially through the adapter element and being received in the threaded holes of the pitch tube for securing the adapter element to the pitch tube.

Abstract translation: 用于风力涡轮机的驱动系统包括至少一个行星齿轮级，可连接到风力涡轮机的转子轴，并包括与多个行星齿轮接合的太阳齿轮，连接到太阳齿轮的从动空心轴，具有 发电机侧端部具有定中心的肩部，并且具有螺纹孔的面侧，所述俯仰管同心地布置到环形齿轮并且轴向地延伸在整个行星台上，适配器元件具有用于接收发电机的开口 在组件中，适配器元件被接纳在发电机侧端部并且径向围绕发电机侧端部并且通过定心台肩沿一个方向固定在一个方向上，螺旋型连接配件轴向地延伸穿过适配器元件并被接收在螺纹 用于将适配器元件固定到俯仰管上的桨距管的孔。

公开(公告)号：US20060234358A1

公开(公告)日：2006-10-19

申请号：US10513424

申请日：2003-04-29

Applicant: Britta Anderlei ,  Georg Sprenger ,  Hermann Sahm ,  Johannes Bongaerts

Inventor： Britta Anderlei ,  Georg Sprenger ,  Hermann Sahm ,  Johannes Bongaerts

IPC: C12P13/22 ,  C12N15/74 ,  C12N1/21

CPC classification number: C12P13/22 ,  C12Y604/01001

Abstract: A process for the microbial production of aromatic amino acids and other metabolites of the aromatic amino acid biosynthetic pathway The invention relates to a process for the microbial production of aromatic amino acids and other metabolites of the aromatic amino acid biosynthetic pathway. Microbially produced substances such as fine chemicals, in particular aromatic amino acids or metabolites of the aromatics biosynthetic pathway, are of great economic interest, and the need for amino acids, for example, continues to increase. The inventors found that, after introducing a pyc gene sequence into microorganisms, it was possible to produce aromatic amino acids and also metabolites of the aromatic biosynthetic pathway in an improved manner. The process of the invention is particularly suitable for producing L-phenylalanine.

Abstract translation: 用于微生物生产芳香族氨基酸和芳香族氨基酸生物合成途径的其它代谢物的方法本发明涉及芳香族氨基酸生物合成途径的芳香族氨基酸等代谢物的微生物生产方法。 微生物物质如精细化学品，特别是芳香族氨基酸或芳族化合物生物合成途径的代谢物具有重大的经济利益，并且氨基酸的需要例如继续增加。 发明人发现，在将pyc基因序列引入微生物之后，可以以改进的方式产生芳香族氨基酸和芳香族生物合成途径的代谢物。 本发明的方法特别适用于生产L-苯丙氨酸。