公开(公告)号：US20210261973A1

公开(公告)日：2021-08-26

申请号：US17307877

申请日：2021-05-04

Applicant: Genentech, Inc.

Inventor： Karthik VEERAVALLI ,  Rebekah MCKENNA

IPC: C12N15/70 ,  C07K16/00 ,  C12N9/90 ,  C12N9/02 ,  C07K16/24 ,  C07K16/22

Abstract: Provided herein are methods and host cells for producing a polypeptide containing two chains, such as an antibody, half-antibody, antibody fragment, or one-armed antibody. The methods and host cells allow for two-chain polypeptide production using expression of polynucleotides encoding the polypeptide chains from extra-chromosomal polynucleotide(s), and expression of one or more chaperone protein(s) (e.g., peptidyl-prolyl isomerases and/or protein disulfide oxidoreductases) from the host cell chromosome using non-native combination(s) of promoters and translational units encoding a chaperone protein.

公开(公告)号：US20180030434A1

公开(公告)日：2018-02-01

申请号：US15782616

申请日：2017-10-12

Applicant: Genentech, Inc.

Inventor： Twyla Noelle LOMBANA ,  Rebekah MCKENNA ,  Christoph SPIESS ,  Karthik VEERAVALLI ,  Michael DILLON

IPC: C12N15/10 ,  G01N33/569 ,  C07K14/195 ,  C07K16/12 ,  G01N33/53 ,  G01N33/68 ,  C12Q1/68

CPC classification number: C12N15/1037 ,  C07K14/195 ,  C07K16/12 ,  C07K16/22 ,  C07K16/244 ,  C07K2317/30 ,  C07K2317/92 ,  C07K2317/94 ,  C07K2319/40 ,  C12Q1/689 ,  G01N33/5306 ,  G01N33/56911 ,  G01N33/6854 ,  G01N33/6857 ,  G01N33/686 ,  G01N2469/20

Abstract: The invention provides methods of identifying bacteria comprising binding polypeptides. The invention also provides methods of identifying bacteria with improved expression of binding polypeptides. The invention also provides methods of identifying binding polypeptides with improved expression. The invention also provides engineered bacteria suitable for use in the methods of the invention. The invention also provides compositions that can be obtained using the methods, for example, anti-interleukin-13 (IL-13) antibodies with improved expression and/or stability. The invention also provides libraries comprising binding polypeptide (e.g., antibody) variants.