公开(公告)号：US20020182673A1

公开(公告)日：2002-12-05

申请号：US10000157

申请日：2001-10-30

Applicant: GENENTECH, INC.

Inventor： Jian Chen ,  Ellen Filvaroff ,  Sherman Fong ,  Dorothy French ,  Audrey Goddard ,  Paul J. Godowski ,  J. Christopher Grimaldi ,  Austin L. Gurney ,  Kenneth J. Hillan ,  Sarah G. Hymowitz ,  Hanzhong Li ,  James Pan ,  Melissa A. Starovasnik ,  Daniel Tumas ,  Menno Van Lookeren ,  Richard Vandlen ,  Colin K. Watanabe ,  P. Mickey Williams ,  William I. Wood ,  Daniel G. Yansura

IPC: C07K014/715 ,  C07H021/04 ,  C12P021/02 ,  C12N005/06

CPC classification number: C07H21/04 ,  C07K16/244

Abstract: The present invention is directed to novel polypeptides having sequence identity with IL-17, IL-17 receptors and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided herein are methods for treating degenerative cartilaginous disorders and other inflammatory diseases.

Abstract translation: 本发明涉及与IL-17，IL-17受体和编码那些多肽的核酸分子具有序列同一性的新型多肽。 本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法 发明。 本文还提供了治疗退行性软骨疾病和其它炎性疾病的方法。

公开(公告)号：US20030073164A1

公开(公告)日：2003-04-17

申请号：US10020786

申请日：2001-12-13

Applicant: GENENTECH, INC.

Inventor： Laura C. Simmons ,  Laura Klimowski ,  Dorothea Reilly ,  Daniel G. Yansura

IPC: C07K016/40 ,  C12P021/02 ,  C12N001/21 ,  C07H021/04

CPC classification number: C07K16/36 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/22 ,  C07K16/2845 ,  C07K16/2878 ,  C07K16/2896 ,  C07K16/32 ,  C07K16/4291 ,  C07K2317/20 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2319/00 ,  C12N15/70

Abstract: The present invention provides methods and compositions for improved expression and production of recombinant antibodies in prokaryotic expression systems. Particularly contemplated are prokaryotic expression and production of full length aglycosylated antibodies. The antibody products of the invention can be used in various aspects of biological research, diagnosis and medical treatment.

Abstract translation: 本发明提供用于改进原核表达系统中重组抗体表达和产生的方法和组合物。 特别考虑的是全长糖基化抗体的原核表达和产生。 本发明的抗体产品可用于生物研究，诊断和治疗的各个方面。

公开(公告)号：US20030109024A1

公开(公告)日：2003-06-12

申请号：US10080866

申请日：2002-02-22

Applicant: GENENTECH, INC.

Inventor： Eriks Sasha Paegle ,  Dorothea Reilly ,  Daniel G. Yansura

IPC: C12N001/12 ,  C12N001/20

CPC classification number: A61K39/0258 ,  C07K14/245 ,  C12N15/70 ,  C12N15/71 ,  C12P21/02 ,  Y02A50/474

Abstract: Vectors for producing polypeptides heterologous to prokaryotes are described comprising, along with the polypeptide-encoding nucleic acid, anti-termination nucleic acid that inhibits intragenic transcription termination with a non-lambda promoter therefor and/or nucleic acid encoding a GreA or GreB protein and a promoter therefor. Also described are processes for producing a heterologous polypeptide in prokaryotic host cells utilizing such elements to improve the quality and/or quantity of heterologous polypeptide produced.

Abstract translation: 描述了用于产生与原核生物异源的多肽的载体，其与编码编码多肽的核酸一起，使用其非λ启动子和/或编码GreA或GreB蛋白的核酸和/或编码GreA或GreB蛋白质的核酸抑制内切转录终止的抗终止核酸 推动者。 还描述了利用这些元件在原核宿主细胞中产生异源多肽以改善产生的异源多肽的质量和/或量的方法。

公开(公告)号：US20030008815A1

公开(公告)日：2003-01-09

申请号：US09747259

申请日：2000-12-20

Applicant: GENENTECH, INC.

Inventor： Jian Chen ,  Ellen Filvaroff ,  Sherman Fong ,  Audrey Goddard ,  Paul J. Godowski ,  Christopher Grimaldi ,  Austin L. Gurney ,  Hanzhong Li ,  Kenneth Hillan ,  Daniel Tumas ,  Menno VanLookeren ,  Richard Vandlen ,  Colin Watanabe ,  P. Mickey Williams ,  William I. Wood ,  Daniel G. Yansura

IPC: A61K038/17 ,  C07H021/04 ,  C12N009/00 ,  C07K014/435 ,  C12P021/02 ,  C12N005/06

CPC classification number: A61K38/17 ,  C07H21/04 ,  C07K14/435 ,  C12N5/06 ,  C12N9/00 ,  C12N15/00 ,  C12P21/02

Abstract: The present invention is directed to novel polypeptides and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention.

公开(公告)号：US20030017592A1

公开(公告)日：2003-01-23

申请号：US09829251

申请日：2001-04-09

Applicant: GENENTECH, INC.

Inventor： Laura C. Simmons ,  Daniel G. Yansura

IPC: C12P021/02 ,  C12N015/87

CPC classification number: C12N15/625 ,  C07K2319/02 ,  C07K2319/036 ,  C07K2319/61 ,  C07K2319/75 ,  C12N15/70

Abstract: The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.

Abstract translation: 本发明公开了意想不到的结果，与野生型信号序列相比，具有降低的翻译强度的突变信号序列提供了基本上完整的处理和高水平的感兴趣的多肽的表达，并且许多哺乳动物多肽需要较窄的翻译水平范围 达到最大分泌。 一组信号序列载体提供一系列翻译强度，以优化感兴趣的多肽的表达。

公开(公告)号：US20020177188A1

公开(公告)日：2002-11-28

申请号：US09874503

申请日：2001-06-05

Applicant: GENENTECH, INC.

Inventor： Jian Chen ,  Ellen Filvaroff ,  Sherman Fong ,  Audrey Goddard ,  Paul J. Godowski ,  J. Christopher Grimaldi ,  Austin Gurney ,  Hanzhong Li ,  Kenneth Hillan ,  Sarah G. Hymowitz ,  Daniel Tumas ,  Melissa A. Starvovasnik ,  Menno Van Lookeren ,  Richard Vandlen ,  Colin Watanabe ,  P. Mickey Williams ,  William I. Wood ,  Daniel G. Yansura

IPC: C07K014/715 ,  C07H021/04 ,  C12P021/02 ,  C12N005/06

CPC classification number: C07H21/04 ,  C07K14/715 ,  C12N5/06 ,  C12P21/02

Abstract: The present invention is directed to novel polypeptides having sequence identity with IL-17, IL-17 receptors and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention and to methods for producing the polypeptides of the present invention. Further provided herein are methods for treating degenerative cartilaginous disorders and other inflammatory diseases.

Abstract translation: 本发明涉及与IL-17，IL-17受体和编码那些多肽的核酸分子具有序列同一性的新型多肽。 本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明的多肽的嵌合多肽分子，与本发明的多肽结合的抗体以及本发明的多肽的制备方法 发明。 本文还提供了治疗退行性软骨疾病和其它炎性疾病的方法。