公开(公告)号：US20030109024A1

公开(公告)日：2003-06-12

申请号：US10080866

申请日：2002-02-22

Applicant: GENENTECH, INC.

Inventor： Eriks Sasha Paegle ,  Dorothea Reilly ,  Daniel G. Yansura

IPC: C12N001/12 ,  C12N001/20

CPC classification number: A61K39/0258 ,  C07K14/245 ,  C12N15/70 ,  C12N15/71 ,  C12P21/02 ,  Y02A50/474

Abstract: Vectors for producing polypeptides heterologous to prokaryotes are described comprising, along with the polypeptide-encoding nucleic acid, anti-termination nucleic acid that inhibits intragenic transcription termination with a non-lambda promoter therefor and/or nucleic acid encoding a GreA or GreB protein and a promoter therefor. Also described are processes for producing a heterologous polypeptide in prokaryotic host cells utilizing such elements to improve the quality and/or quantity of heterologous polypeptide produced.

Abstract translation: 描述了用于产生与原核生物异源的多肽的载体，其与编码编码多肽的核酸一起，使用其非λ启动子和/或编码GreA或GreB蛋白的核酸和/或编码GreA或GreB蛋白质的核酸抑制内切转录终止的抗终止核酸 推动者。 还描述了利用这些元件在原核宿主细胞中产生异源多肽以改善产生的异源多肽的质量和/或量的方法。

公开(公告)号：US20030073164A1

公开(公告)日：2003-04-17

申请号：US10020786

申请日：2001-12-13

Applicant: GENENTECH, INC.

Inventor： Laura C. Simmons ,  Laura Klimowski ,  Dorothea Reilly ,  Daniel G. Yansura

IPC: C07K016/40 ,  C12P021/02 ,  C12N001/21 ,  C07H021/04

CPC classification number: C07K16/36 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/22 ,  C07K16/2845 ,  C07K16/2878 ,  C07K16/2896 ,  C07K16/32 ,  C07K16/4291 ,  C07K2317/20 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/55 ,  C07K2319/00 ,  C12N15/70

Abstract: The present invention provides methods and compositions for improved expression and production of recombinant antibodies in prokaryotic expression systems. Particularly contemplated are prokaryotic expression and production of full length aglycosylated antibodies. The antibody products of the invention can be used in various aspects of biological research, diagnosis and medical treatment.

Abstract translation: 本发明提供用于改进原核表达系统中重组抗体表达和产生的方法和组合物。 特别考虑的是全长糖基化抗体的原核表达和产生。 本发明的抗体产品可用于生物研究，诊断和治疗的各个方面。