公开(公告)号：US20030109024A1

公开(公告)日：2003-06-12

申请号：US10080866

申请日：2002-02-22

Applicant: GENENTECH, INC.

Inventor： Eriks Sasha Paegle ,  Dorothea Reilly ,  Daniel G. Yansura

IPC: C12N001/12 ,  C12N001/20

CPC classification number: A61K39/0258 ,  C07K14/245 ,  C12N15/70 ,  C12N15/71 ,  C12P21/02 ,  Y02A50/474

Abstract: Vectors for producing polypeptides heterologous to prokaryotes are described comprising, along with the polypeptide-encoding nucleic acid, anti-termination nucleic acid that inhibits intragenic transcription termination with a non-lambda promoter therefor and/or nucleic acid encoding a GreA or GreB protein and a promoter therefor. Also described are processes for producing a heterologous polypeptide in prokaryotic host cells utilizing such elements to improve the quality and/or quantity of heterologous polypeptide produced.

Abstract translation: 描述了用于产生与原核生物异源的多肽的载体，其与编码编码多肽的核酸一起，使用其非λ启动子和/或编码GreA或GreB蛋白的核酸和/或编码GreA或GreB蛋白质的核酸抑制内切转录终止的抗终止核酸 推动者。 还描述了利用这些元件在原核宿主细胞中产生异源多肽以改善产生的异源多肽的质量和/或量的方法。