公开(公告)号：US10226770B2

公开(公告)日：2019-03-12

申请号：US14864491

申请日：2015-09-24

申请人： Fluidigm Corporation

发明人： Jake Kimball ,  Brandon Ripley ,  Gang Sun ,  Dominique Toppani ,  Myo Thu Maung

IPC分类号： C12Q3/00 ,  B01L7/00 ,  B01L3/00 ,  C12P19/34 ,  G01N21/64 ,  G01N21/76

摘要： A thermal cycler for a microfluidic device includes a controller operable to provide a series of electrical signals, a heat sink, and a heating element in thermal communication with the heat sink and operable to receive the series of electrical signals from the controller. The thermal cycler also includes a thermal chuck in thermal communication with the heating element. The thermal chuck comprises a heating surface operable to make thermal contact with the microfluidic device. The heating surface is characterized by a temperature ramp rate between 2.5 degrees Celsius per second and 5.5 degrees Celsius per second and a temperature difference between a first portion of the heating surface supporting a first portion of the microfluidic device and a second portion of the heating surface supporting a second portion of the microfluidic device is less than 0.25° C.

公开(公告)号：US20160114327A1

公开(公告)日：2016-04-28

申请号：US14864491

申请日：2015-09-24

申请人： Fluidigm Corporation

发明人： Jake Kimball ,  Brandon Ripley ,  Gang Sun ,  Dominique Toppani ,  Myo Thu Maung

IPC分类号： B01L7/00

CPC分类号： B01L7/52 ,  B01L3/5027 ,  B01L2200/025 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/0893 ,  B01L2300/1805 ,  B01L2300/1822 ,  C12P19/34 ,  C12Q3/00 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/76

摘要： A thermal cycler for a microfluidic device includes a controller operable to provide a series of electrical signals, a heat sink, and a heating element in thermal communication with the heat sink and operable to receive the series of electrical signals from the controller. The thermal cycler also includes a thermal chuck in thermal communication with the heating element. The thermal chuck comprises a heating surface operable to make thermal contact with the microfluidic device. The heating surface is characterized by a temperature ramp rate between 2.5 degrees Celsius per second and 5.5 degrees Celsius per second and a temperature difference between a first portion of the heating surface supporting a first portion of the microfluidic device and a second portion of the heating surface supporting a second portion of the microfluidic device is less than 0.25° C.

摘要翻译： 用于微流体装置的热循环仪包括控制器，其可操作以提供与散热器热连通的一系列电信号，散热器和加热元件，并可操作以从控制器接收一系列电信号。 热循环仪还包括与加热元件热连通的热卡盘。 热卡盘包括可操作以与微流体装置进行热接触的加热表面。 加热表面的特征在于每秒2.5摄氏度和5.5摄氏度/秒之间的温度升高速率，并且支撑微流体装置的第一部分的加热表面的第一部分与加热表面的第二部分之间的温度差 支撑微流体装置的第二部分小于0.25℃

公开(公告)号：US20190217302A1

公开(公告)日：2019-07-18

申请号：US16245537

申请日：2019-01-11

申请人： Fluidigm Corporation

发明人： Jake Kimball ,  Brandon Ripley ,  Gang Sun ,  Dominique Toppani ,  Myo Thu Maung

IPC分类号： B01L7/00 ,  C12Q3/00 ,  C12P19/34

CPC分类号： B01L7/52 ,  B01L3/5027 ,  B01L2200/025 ,  B01L2200/147 ,  B01L2300/0819 ,  B01L2300/0893 ,  B01L2300/1805 ,  B01L2300/1822 ,  C12P19/34 ,  C12Q3/00 ,  G01N21/6408 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/76

摘要： A thermal cycler for a microfluidic device includes a controller operable to provide a series of electrical signals, a heat sink, and a heating element in thermal communication with the heat sink and operable to receive the series of electrical signals from the controller. The thermal cycler also includes a thermal chuck in thermal communication with the heating element. The thermal chuck comprises a heating surface operable to make thermal contact with the microfluidic device. The heating surface is characterized by a temperature ramp rate between 2.5 degrees Celsius per second and 5.5 degrees Celsius per second and a temperature difference between a first portion of the heating surface supporting a first portion of the microfluidic device and a second portion of the heating surface supporting a second portion of the microfluidic device is less than 0.25° C.