-
公开(公告)号：US11459540B2
公开(公告)日：2022-10-04
申请号：US15983823
申请日：2018-05-18
发明人： Rui Tostoes , Bart Lipkens , Kedar Chitale , Benjamin Ross-Johnsrud , Walter M. Presz, Jr. , Jack Saloio
摘要： Separation of materials is achieved using affinity binding and acoustophoretic techniques. A column provided with a fluid mixture of materials for separation and support structures may be used with acoustic waves to block flow of the support structures. The support structures can have an affinity for one or more materials in the fluid mixture. By blocking flow of the support structures, materials bound or adhered to the support structure are also blocked.
-
公开(公告)号：US11179747B2
公开(公告)日：2021-11-23
申请号：US15206244
申请日：2016-07-09
发明人： Bart Lipkens , Walter M Presz, Jr. , Kedar Chitale , Thomas J Kennedy, III , Rudolf Gilmanshin , Dane Mealey , Brian Dutra , David Sokolowski
摘要： An acoustophoretic device is disclosed. The acoustophoretic device includes an acoustic chamber, an ultrasonic transducer, and a reflector. The ultrasonic transducer includes a piezoelectric material driven by a voltage signal to create a multi-dimensional acoustic standing wave in the acoustic chamber emanating from a non-planar face of the piezoelectric material. A method for separating a second fluid or a particulate from a host fluid is also disclosed. The method includes flowing the mixture through an acoustophoretic device. A voltage signal is sent to drive the ultrasonic transducer to create the multi-dimensional acoustic standing wave in the acoustic chamber such that the second fluid or particulate is continuously trapped in the standing wave, and then agglomerates, aggregates, clumps, or coalesces together, and subsequently rises or settles out of the host fluid due to buoyancy or gravity forces, and exits the acoustic chamber.
-
公开(公告)号：US20200308533A1
公开(公告)日：2020-10-01
申请号：US16880787
申请日：2020-05-21
发明人： Bart Lipkens , Erik Miller , Benjamin Ross-Johnsrud , Walter M. Presz, JR. , Kedar Chitale , Thomas J. Kennedy, III , Lauryn Winiarski
摘要： Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.
-
公开(公告)号：USD797887S1
公开(公告)日：2017-09-19
申请号：US29562478
申请日：2016-04-26
-
公开(公告)号：US20170217794A1
公开(公告)日：2017-08-03
申请号：US15490878
申请日：2017-04-18
发明人： Bart Lipkens , Walter M. Presz, JR. , Kedar Chitale , Brian McCarthy , Benjamin Ross-Johnsrud , Thomas J. Kennedy, III , Dane Mealey , Brian Dutra , David Sokolowski
CPC分类号： C02F1/36 , B01D17/04 , B01D17/041 , B01D17/044 , B01D19/0078 , B01D21/28 , B06B1/0622 , B06B1/0644 , C02F1/40 , C12M35/04 , C12M47/02 , C12N1/02 , C12N13/00 , G10K11/28 , H01L41/0913
摘要： Separation of particles or droplets from a host fluid may be achieved using a transducer and/or reflector that is a thin, non-planar structure. The thin non-planar structure improves operation of an acoustic standing wave generated by an acoustic transducer. The structure may operate as a pressure release boundary and may be constructed as plastic film.
-
公开(公告)号：US09663756B1
公开(公告)日：2017-05-30
申请号：US15053359
申请日：2016-02-25
CPC分类号： C12M47/04 , C12M33/00 , C12M33/08 , C12M47/02 , C12N5/0062 , C12N13/00 , C12N2527/00
摘要： Methods, systems, and apparatus for acoustic separation of cellular supporting materials such as microcarriers or microbubbles from cell culture are provided. In one aspect, a method includes flowing a fluid containing the cellular supporting material and the cells through a flow chamber; driving at least one acoustic transducer to launch an acoustic wave from the acoustic transducer positioned on a first wall of the flow chamber to a reflector positioned on a second wall of the flow chamber to create, in the flow chamber, a multi-dimensional field that includes first spatial locales where acoustic pressure amplitude is elevated from a baseline level when the acoustic transducer is turned off, and second spatial locales where acoustic pressure amplitude is substantially identical to the baseline level when the acoustic transducer is turned off, wherein the first wall is opposite to the second wall; preferentially trapping, and gravity separating, the cellular supporting material or the cells at the first or second spatial locales inside the flow chamber; and collecting cells separated from the cellular supporting material using a first collection duct coupled to the flow chamber.
-
公开(公告)号：US10322949B2
公开(公告)日：2019-06-18
申请号：US15490878
申请日：2017-04-18
发明人： Bart Lipkens , Walter M. Presz, Jr. , Kedar Chitale , Brian McCarthy , Benjamin Ross-Johnsrud , Thomas J. Kennedy, III , Dane Mealey , Brian Dutra , David Sokolowski
IPC分类号： C02F1/36 , B06B1/06 , B01D17/04 , C02F1/40 , B01D19/00 , B01D21/28 , C12M1/42 , G10K11/28 , C12N1/02 , C12N13/00 , C12M1/00 , H01L41/09
摘要： Separation of particles or droplets from a host fluid may be achieved using a transducer and/or reflector that is a thin, non-planar structure. The thin non-planar structure improves operation of an acoustic standing wave generated by an acoustic transducer. The structure may operate as a pressure release boundary and may be constructed as plastic film.
-
公开(公告)号：US20180362918A1
公开(公告)日：2018-12-20
申请号：US16010296
申请日：2018-06-15
发明人： Bart Lipkens , Rui Tostoes , Brian Dutra , Rudolf Gilmanshin , Jason Dionne , Walter M. Presz, JR. , Benjamin Ross-Johnsrud , Goutam Ghoshal , Kedar Chitale
摘要： A closed and modular fluidic system composed of one or more acoustic elements and cell processing reagents. The system employs a cellular manufacturing process for producing cell and gene therapy therapeutics.
-
公开(公告)号：US20180015392A1
公开(公告)日：2018-01-18
申请号：US15690263
申请日：2017-08-29
发明人： Bart Lipkens , Walter M. Presz, JR. , Jeffrey King , Jason Barnes , Dane Mealey , Brian McCarthy , Ben Ross-Johnsrud , Kedar Chitale
摘要： Devices for separating a host fluid from a second fluid or particulate are disclosed. The devices include an acoustic chamber, a fluid outlet at a top end of the acoustic chamber, a concentrate outlet at a bottom end of the acoustic chamber, and an inlet on a first side end of the acoustic chamber. An ultrasonic transducer and reflector create a multi-dimensional acoustic standing wave in the acoustic chamber that traps and separates particulates (e.g. cells) from a host fluid. The host fluid is collected via the fluid outlet, and the particulates are collected via the concentrate outlet. The device is a large-scale device that is able to process liters/hour, and has a large interior volume.
-
公开(公告)号：US20180010085A1
公开(公告)日：2018-01-11
申请号：US15696176
申请日：2017-09-05
发明人： Bart Lipkens , Erik Miller , Benjamin Ross-Johnsrud , Walter M. Presz , Kedar Chitale , Thomas J. Kennedy, III , Lauren Winiarski
IPC分类号： C12M1/00 , C12M1/42 , C12M1/26 , B06B1/06 , B01D21/28 , B01D17/06 , C12N13/00 , B01D17/04 , H01L41/09
摘要： Acoustic perfusion devices for separating biological cells from other material in a fluid mixture are disclosed. The devices include an inlet port, an outlet port, and a collection port that are connected to an acoustic chamber. An ultrasonic transducer creates an acoustic standing wave in the acoustic chamber that permits a continuous flow of fluid to be recovered through the collection port while keeping the biological cells within the acoustic chamber to be returned to the bioreactor from which the fluid mixture is being drawn.
-
-
-
-
-
-
-
-
-
搜索结果
38 条记录 (耗时 0.024 秒)
