知搜-全球专利检索

  1. 登录
  2. 注册

搜索结果

31 条记录 (耗时 0.029 秒)

    Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange
    1.
    发明申请
    Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange 审中-公开
    提高位点特异性多核苷酸交换效率的方法和组合

    公开(公告)号：US20070143881A1

    公开(公告)日：2007-06-21

    申请号：US11427947

    申请日：2006-06-30

    申请人： Dennis L. Bidney ,  Sheila E. Maddock ,  Xinli E. Wu

    发明人： Dennis L. Bidney ,  Sheila E. Maddock ,  Xinli E. Wu

    IPC分类号： A01H1/00

    CPC分类号： C12N15/8213 ,  C12N15/8205 ,  C12N15/8207

    摘要： Methods and compositions using a site-specific integration system are combined with methods and compositions which deliver compositions via microinjection directly to the embryo sac of a plant. The methods allow for various components of the site-specific recombination system to be introduced into the cellular environment of the embryo sac a composition comprising at least one component of the site-specific recombination system is injected into an embryo sac, providing improved efficiency of expression, recombination, integration, exchange, excision and/or inversion of a polynucleotide of interest. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and tissues, plant parts, and/or plants produced therefrom. Cells, egg cells, zygotes, embryos, endosperm, tissues, seeds, and/or plants produced by the methods and comprising the polynucleotide(s) of interest are also provided.

    摘要翻译： 使用位点特异性整合系统的方法和组合物与通过显微注射将组合物直接递送至植物胚囊的方法和组合物组合。 所述方法允许将位点特异性重组系统的各种组分引入胚囊的细胞环境中，将包含位点特异性重组系统的至少一个组分的组合物注入胚囊，提供更好的表达效率 ，重组，整合，交换，切除和/或转化感兴趣的多核苷酸。 感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳以及由其产生的组织，植物部分和/或植物的基因组中。 还提供了通过方法产生并包含感兴趣的多核苷酸的细胞，卵细胞，合子，胚胎，胚乳，组织，种子和/或植物。

    Methods for Introducing Into a Plant a Polynucleotide of Interest
    2.
    发明申请
    Methods for Introducing Into a Plant a Polynucleotide of Interest 审中-公开
    向植物引入感兴趣的多核苷酸的方法

    公开(公告)号：US20070143880A1

    公开(公告)日：2007-06-21

    申请号：US11427371

    申请日：2006-06-29

    申请人： Dennis L. Bidney ,  Sheila E. Maddock ,  Xinli E. Wu ,  Zuo-Yu Zhao

    发明人： Dennis L. Bidney ,  Sheila E. Maddock ,  Xinli E. Wu ,  Zuo-Yu Zhao

    IPC分类号： A01H1/00 ,  C12N15/82

    CPC分类号： C12N15/8205 ,  C12N15/8207 ,  C12N15/8213

    摘要： The present invention provides methods and compositions which deliver Agrobacterium via microinjection directly into the embryo sac. At the time of injection, the embryo sac can comprise an egg cell, or alternatively, the embryo sac can be fertilized and comprise either a zygote or an embryo. Once inside the embryo sac, the Agrobacterium harboring a T-DNA having a polynucleotide of interest can express of the polynucleotide of interest in the plant. Further, the Agrobacterium can transfer the T-DNA having the polynucleotide of interest to the plant nucleus to produce a transformed plant. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and any tissue, plant part, and/or plant generated therefrom.

    摘要翻译： 本发明提供了通过显微注射将土壤杆菌直接递送到胚囊中的方法和组合物。 在注射时，胚囊可以包括卵细胞，或者，胚囊可被受精并且包括受精卵或胚胎。 一旦进入胚囊，携带具有感兴趣的多核苷酸的T-DNA的农杆菌可以在植物中表达感兴趣的多核苷酸。 此外，农杆菌可以将具有目标多核苷酸的T-DNA转移到植物核，以产生转化的植物。 感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳的基因组中，以及由其产生的任何组织，植物部分和/或植物。

    Method of plant transformation
    8.
    发明授权
    Method of plant transformation 有权
    植物转化方法

    公开(公告)号：US06265638B1

    公开(公告)日：2001-07-24

    申请号：US09407574

    申请日：1999-09-28

    申请人： Dennis L. Bidney ,  Christopher Jay Scelonge

    发明人： Dennis L. Bidney ,  Christopher Jay Scelonge

    IPC分类号： C12N1582

    CPC分类号： C12N15/8241 ,  C12N15/8205 ,  C12N15/8209

    摘要： Compositions and methods for the efficient co-transformation of a plant are provided. Novel compositions are Agrobacterium strains that have been engineered to comprise at least two binary vector plasmids in addition to a helper plasmid comprising the vir functions. Each of the binary vectors comprises its own T-DNA borders flanking a heterologous nucleotide sequence of interest. Methods of the invention comprise the use of these novel multiple-binary vector Agrobacterium strains to co-transform a plant. In this manner, heterologous nucleotide sequences of interest residing on different binary vectors can be independently introduced into the plant in a single transformation event and incorporated in the plant's nuclear DNA in an unlinked manner. The invention also provides for regenerated, fertile transgenic plants, transgenic seeds produced therefrom, and T1 and subsequent generations.

    摘要翻译： 提供了植物有效共同转化的组合物和方法。 除了包含vir功能的辅助质粒之外，新型组合物是经过工程改造以包含至少两种二元载体质粒的农杆菌菌株。 每个二元载体包含在感兴趣的异源核苷酸序列侧翼的其自身的T-DNA边界。 本发明的方法包括使用这些新型多元二元载体农杆菌菌株共同转化植物。 以这种方式，驻留在不同二元载体上的感兴趣的异源核苷酸序列可以在单个转化事件中独立地引入植物中，并以非连接的方式并入植物的核DNA中。 本发明还提供了再生的，可育的转基因植物，由其产生的转基因种子，以及T1和随后的几代。

    FRT recombination sites and methods of use
    10.
    发明授权
    FRT recombination sites and methods of use 有权
    FRT重组位点和使用方法

    公开(公告)号：US08318493B2

    公开(公告)日：2012-11-27

    申请号：US12756211

    申请日：2010-04-08

    申请人： Yumin Tao ,  Dennis L. Bidney ,  William J. Gordon-Kamm ,  Leszek A. Lyznik

    发明人： Yumin Tao ,  Dennis L. Bidney ,  William J. Gordon-Kamm ,  Leszek A. Lyznik

    IPC分类号： C12N15/87 ,  C12N15/82

    CPC分类号： C12N15/8213 ,  C12N15/102 ,  C12N15/1051 ,  C12N15/1093 ,  C40B40/08

    摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.

    摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。