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    Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange
    1.
    发明申请
    Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange 审中-公开
    提高位点特异性多核苷酸交换效率的方法和组合

    公开(公告)号：US20070143881A1

    公开(公告)日：2007-06-21

    申请号：US11427947

    申请日：2006-06-30

    申请人： Dennis L. Bidney ,  Sheila E. Maddock ,  Xinli E. Wu

    发明人： Dennis L. Bidney ,  Sheila E. Maddock ,  Xinli E. Wu

    IPC分类号： A01H1/00

    CPC分类号： C12N15/8213 ,  C12N15/8205 ,  C12N15/8207

    摘要： Methods and compositions using a site-specific integration system are combined with methods and compositions which deliver compositions via microinjection directly to the embryo sac of a plant. The methods allow for various components of the site-specific recombination system to be introduced into the cellular environment of the embryo sac a composition comprising at least one component of the site-specific recombination system is injected into an embryo sac, providing improved efficiency of expression, recombination, integration, exchange, excision and/or inversion of a polynucleotide of interest. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and tissues, plant parts, and/or plants produced therefrom. Cells, egg cells, zygotes, embryos, endosperm, tissues, seeds, and/or plants produced by the methods and comprising the polynucleotide(s) of interest are also provided.

    摘要翻译： 使用位点特异性整合系统的方法和组合物与通过显微注射将组合物直接递送至植物胚囊的方法和组合物组合。 所述方法允许将位点特异性重组系统的各种组分引入胚囊的细胞环境中，将包含位点特异性重组系统的至少一个组分的组合物注入胚囊，提供更好的表达效率 ，重组，整合，交换，切除和/或转化感兴趣的多核苷酸。 感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳以及由其产生的组织，植物部分和/或植物的基因组中。 还提供了通过方法产生并包含感兴趣的多核苷酸的细胞，卵细胞，合子，胚胎，胚乳，组织，种子和/或植物。

    Methods for Introducing Into a Plant a Polynucleotide of Interest
    2.
    发明申请
    Methods for Introducing Into a Plant a Polynucleotide of Interest 审中-公开
    向植物引入感兴趣的多核苷酸的方法

    公开(公告)号：US20070143880A1

    公开(公告)日：2007-06-21

    申请号：US11427371

    申请日：2006-06-29

    申请人： Dennis L. Bidney ,  Sheila E. Maddock ,  Xinli E. Wu ,  Zuo-Yu Zhao

    发明人： Dennis L. Bidney ,  Sheila E. Maddock ,  Xinli E. Wu ,  Zuo-Yu Zhao

    IPC分类号： A01H1/00 ,  C12N15/82

    CPC分类号： C12N15/8205 ,  C12N15/8207 ,  C12N15/8213

    摘要： The present invention provides methods and compositions which deliver Agrobacterium via microinjection directly into the embryo sac. At the time of injection, the embryo sac can comprise an egg cell, or alternatively, the embryo sac can be fertilized and comprise either a zygote or an embryo. Once inside the embryo sac, the Agrobacterium harboring a T-DNA having a polynucleotide of interest can express of the polynucleotide of interest in the plant. Further, the Agrobacterium can transfer the T-DNA having the polynucleotide of interest to the plant nucleus to produce a transformed plant. The polynucleotide of interest may be stably integrated into the genome of the egg cell, zygote, embryo, or endosperm, and any tissue, plant part, and/or plant generated therefrom.

    摘要翻译： 本发明提供了通过显微注射将土壤杆菌直接递送到胚囊中的方法和组合物。 在注射时，胚囊可以包括卵细胞，或者，胚囊可被受精并且包括受精卵或胚胎。 一旦进入胚囊，携带具有感兴趣的多核苷酸的T-DNA的农杆菌可以在植物中表达感兴趣的多核苷酸。 此外，农杆菌可以将具有目标多核苷酸的T-DNA转移到植物核，以产生转化的植物。 感兴趣的多核苷酸可以稳定地整合到卵细胞，合子，胚或胚乳的基因组中，以及由其产生的任何组织，植物部分和/或植物。

    MAR/SAR elements flanking RSYN7-driven construct
    8.
    发明授权
    MAR/SAR elements flanking RSYN7-driven construct 失效
    MAR / SAR元素位于RSYN7驱动构建体的侧翼

    公开(公告)号：US06388066B1

    公开(公告)日：2002-05-14

    申请号：US09405779

    申请日：1999-09-27

    申请人： Wesley B. Bruce ,  Sheila E. Maddock

    发明人： Wesley B. Bruce ,  Sheila E. Maddock

    IPC分类号： C07H2104

    CPC分类号： C12N15/8216 ,  C12N15/822 ,  C12N15/8222 ,  C12N15/8227 ,  C12N15/8234

    摘要： This invention discloses to compositions and methods for altering the characteristic pattern of expression associated with a promoter. More particularly the constitutive expression pattern associated with the small synthetic promoter, Rsyn7 is modified so that expression of a heterologous nucleotide sequence operably linked to the Rsyn7 promoter is in a tissue localized manner. This modification of the Rsyn7 pattern of expression occurs as a result of the addition of matrix attachment region DNA sequences to the flanks or 5′ and 3′ ends of an expression cassette comprising the Rsyn7 promoter operably linked to a heterologous nucleotide sequence of interest. DNA constructs, transformed plant cells and transformed plants are provided.

    摘要翻译： 本发明公开了用于改变与启动子相关的表达特征模式的组合物和方法。 更具体地，与小合成启动子Rsyn7相关的组成型表达模式被修饰，使得可操作地连接到Rsyn7启动子的异源核苷酸序列的表达是组织定位的。 Rsyn7模式表达的这种修饰是由于将基质连接区DNA序列添加到包含可操作地连接到感兴趣的异源核苷酸序列的Rsyn7启动子的表达盒的侧翼或5'和3'末端而进行的。 提供了DNA构建体，转化的植物细胞和转化的植物。