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公开(公告)号：US20170253937A1
公开(公告)日：2017-09-07
申请号：US15376495
申请日：2016-12-12
发明人： Xudong Ye , Larry A. Gilbertson , Shihshieh Huang , Susan J. Johnson , Michael W. Petersen , David Walters , Paul S. Chomet
CPC分类号： C12N15/8265 , C12N15/8205 , C12N15/8209 , C12N15/821 , C12N15/8212 , C12N15/8218 , C12N15/8222 , C12N15/8231 , C12N15/8234 , C12N15/8261 , C12N15/8263 , C12N15/8267 , C12N15/8287 , C12N15/8289 , C12N15/829 , C12N15/8294
摘要： The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
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2.发明申请REPRODUCTION OF FEMALE STERILITY LINES AND ITS APPLICATION IN HYBRID SEED PRODUCTION 审中-公开女性不育系的再生及其在混合种子生产中的应用公开(公告)号：US20160326536A1
公开(公告)日：2016-11-10
申请号：US15109540
申请日：2014-12-29
发明人： Ning WEI , Haodong CHEN , Zhongcheng LIANG
IPC分类号： C12N15/82
CPC分类号： C12N15/829 , A01H1/02 , C07K14/415 , C12N15/8287 , C12N15/8289
摘要： Provided in the present invention is a method for preparing hybrid rice, comprising the reproduction and maintenance of the female sterility line and the use thereof in preparing hybrid rice.
摘要翻译： 在本发明中提供了一种制备杂交稻的方法，包括雌性不育系的繁殖和维持及其在制备杂交稻中的用途。
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公开(公告)号：US08946507B2
公开(公告)日：2015-02-03
申请号：US12819702
申请日：2010-06-21
申请人： Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
发明人： Timothy Robert Hawkes , Glynn Mitchell , Stephen Thomas Hadfield , Paul Anthony Thompson , Russell Viner , Yan Zhang
CPC分类号： C12N9/0024 , C12N9/18 , C12N15/8287 , C12N15/8289 , C12N15/829 , C12P13/04 , C12P41/007
摘要： A method of producing male or female sterile plants comprising the steps of transforming plant material with a polynucleotide which encodes at least one enzyme which reacts with a non-phytotoxic substance to produce a phytotoxic one, and regenerating the thus transformed material into a plant, wherein the said non-phytotoxic substance is applied to the plant up to the time of male or female gamete formation and/or maturation, so that the non-phytotoxic substance provides for the production of a phytotoxic one which selectively prevents the formation of or otherwise renders the said gametes non-functional, wherein the enzyme is expressed preferentially in either male or female reproductive structures, characterised in that (i) the non-phytotoxic substance is D-phosphinothricin and (ii) the enzyme is a D-amino acid oxidase.
摘要翻译： 一种产生雄性或雌性不育植物的方法，包括以编码与非植物毒性物质反应产生植物毒性物质的至少一种酶的多核苷酸转化植物材料并将由此转化的材料再生成植物的步骤，其中 所述非植物毒性物质直到雄性或雌性配子形成和/或成熟时被施用于植物，使得非植物毒性物质提供了用于选择性地防止形成或以其它方式形成的植物毒性物质 所述配子是非功能性的，其中所述酶优先在雄性或雌性生殖结构中表达，其特征在于（i）非植物毒性物质是D-膦丝菌素，和（ii）所述酶是D-氨基酸氧化酶。
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公开(公告)号：US20140245490A1
公开(公告)日：2014-08-28
申请号：US14119426
申请日：2012-05-31
IPC分类号： C12N15/82
CPC分类号： C12N15/8287 , C07K14/415 , C12N15/8249 , C12N15/8261 , C12N15/829 , Y02A40/146
摘要： Plants having modulated expression of a HAF or BEE polypeptide and which modulate aspects of plant fertility, such as parthenocarpy, fertilization efficiency, and/or fruit size, are described. Methods of modulating fertility characteristics in a plant by modulating the expression of a HAF or BEE polypeptide in the plant are also described.
摘要翻译： 描述了调节HAF或BEE多肽表达并调节植物生育力方面的植物，如单性结实，受精效率和/或果实大小。 还描述了通过调节植物中HAF或BEE多肽的表达来调节植物中生育力特征的方法。
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公开(公告)号：US20130091603A1
公开(公告)日：2013-04-11
申请号：US13242193
申请日：2011-09-23
IPC分类号： C12N15/82
CPC分类号： C12N15/8289 , C07K14/415 , C12N9/22 , C12N15/8205 , C12N15/823 , C12N15/8231 , C12N15/8233 , C12N15/8234 , C12N15/8263 , C12N15/827 , C12N15/829
摘要： The present invention relates to the regulation of reproductive development, particularly to the genetic ablation of reproductive tissues in angiosperm and gymnosperm species. Reproductive-preferred promoters, regulatory elements, and cytotoxic nucleotide sequences are disclosed herein, as are constructs and methods for genetic ablation.
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公开(公告)号：US08293975B2
公开(公告)日：2012-10-23
申请号：US12061834
申请日：2008-04-03
申请人： Andrew Mark Cigan , Erica Unger
发明人： Andrew Mark Cigan , Erica Unger
CPC分类号： C12N15/8218 , A01H1/02 , C12N15/8212 , C12N15/8216 , C12N15/8231 , C12N15/8263 , C12N15/8287 , C12N15/8289 , C12N15/829
摘要： Methods are provided in which a promoter hairpin construct is introduced into a cell to suppress expression of a gene of interest; a change in phenotype of the cell, or of an organism regenerated from the cell, is detected; and the change in phenotype identifies the function of a product of the gene of interest. Also provided are methods for restoring the original phenotype.
摘要翻译： 提供了将促进剂发夹构建体导入细胞以抑制目的基因表达的方法; 检测细胞表型或从细胞再生的生物体的变化; 并且表型的变化确定了感兴趣的基因的产物的功能。 还提供了恢复原始表型的方法。
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公开(公告)号：US20110302671A1
公开(公告)日：2011-12-08
申请号：US12940581
申请日：2010-11-05
申请人： Thomas Dresselhaus , Simone Cordts , Suseno Amien , Horst Loerz
发明人： Thomas Dresselhaus , Simone Cordts , Suseno Amien , Horst Loerz
IPC分类号： A01H1/00 , C12N15/74 , C12N5/10 , A01H5/10 , C12N1/19 , C12N15/63 , C07H21/04 , A01H5/00 , C12N15/82 , C12N1/21
CPC分类号： C12N15/8287 , C07K14/415 , C12N15/8234 , C12N15/8261 , C12N15/829 , Y02A40/146
摘要： The present invention relates to isolated nucleotide sequences useful for the production of plants with a modified embryo sac, embryo and or endosperm development, and to transgenic cells and plants transformed with the nucleotide sequences.
摘要翻译： 本发明涉及可用于生产具有修饰的胚囊，胚和/或胚乳发育的植物的分离的核苷酸序列，以及用该核苷酸序列转化的转基因细胞和植物。
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公开(公告)号：US20110258738A1
公开(公告)日：2011-10-20
申请号：US13138005
申请日：2009-12-22
申请人： Raju Datla , Maozhi Ren , Shuqing Qiu , Gopalan Selvaraj
发明人： Raju Datla , Maozhi Ren , Shuqing Qiu , Gopalan Selvaraj
CPC分类号： G01N33/5097 , C12N9/1205 , C12N15/825 , C12N15/8261 , C12N15/8262 , C12N15/827 , C12N15/8273 , C12N15/8289 , C12N15/829 , Y02A40/146
摘要： Broad experimental tools that include biochemical molecular developmental global genomics and loss and gain of function transgenic approaches have been applied to address target of rapamycin (TOR) signaling pathway in plants especially using Arabidopsis model system and Brassica napus crop Towards this objective, putative TOR interacting proteins (TIPs) have been identified and functions of these implicated in diverse developmental and biochemical processes have been investigated Functional studies including over-expression and silencing of TIPs have shown a range of phenotypes that include nutrition-use-efficiency, altered plant architecture and stress resistance in transgenic Arabidopsis and Brassica lines Some of these phenotypes are relevant to important developmental pathways implicated in canola crop yield and performance
摘要翻译： 包括生物化学分子发育全球基因组学和功能转基因方法的损失和增益的广泛实验工具已被应用于解决植物中雷帕霉素（TOR）信号通路的靶标，特别是使用拟南芥模拟系统和甘蓝型油菜作物。针对这一目标，推定的TOR相互作用蛋白 （TIPs）已经被鉴定，并且涉及多种发育和生物化学过程的功能已经被研究了包括过度表达和沉默的TIP的功能研究已经显示了一系列表型，包括营养 - 使用效率，改变的植物结构和抗压力 在转基因拟南芥和芸苔系中有些这些表型与涉及卡诺拉作物产量和性能的重要发育途径相关
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公开(公告)号：US07915398B2
公开(公告)日：2011-03-29
申请号：US12057862
申请日：2008-03-28
申请人： Timothy W Fox , Yongzhong Wu
发明人： Timothy W Fox , Yongzhong Wu
CPC分类号： C12N15/8218 , A01H1/02 , C12N15/8212 , C12N15/8216 , C12N15/8231 , C12N15/8263 , C12N15/8287 , C12N15/8289 , C12N15/829
摘要： A promoter isolated from Zea mays, designated the P67 promoter, provides a high level of specificity for expression in developing pollen, particularly at the mid-uninucleate stage, as confirmed by RT-PCR and Northern blot analyses of RNA samples from various tissues. Compositions and methods of use of the P67 promoter are disclosed.
摘要翻译： 分离自Zea mays的启动子，被命名为P67启动子，为开发花粉的表达提供了高水平的特异性，特别是在非核中期阶段，通过来自各种组织的RNA样品的RT-PCR和Northern印迹分析证实。 公开了P67启动子的组合物和使用方法。
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公开(公告)号：US07851679B2
公开(公告)日：2010-12-14
申请号：US12180180
申请日：2008-07-25
CPC分类号： C12N15/8289 , C07K14/415 , C12N9/22 , C12N15/8205 , C12N15/823 , C12N15/8231 , C12N15/8233 , C12N15/8234 , C12N15/8263 , C12N15/827 , C12N15/829
摘要： The present invention relates to the regulation of reproductive development, particularly to the genetic ablation of reproductive tissues in angiosperm and gymnosperm species. Reproductive-preferred promoters, regulatory elements, and cytotoxic nucleotide sequences are disclosed herein, as are constructs and methods for genetic ablation.
摘要翻译： 本发明涉及生殖发育的调节，特别涉及被子植物和裸子植物物种中生殖组织的遗传消融。 生殖优选的启动子，调节元件和细胞毒性核苷酸序列在本文中公开，以及用于遗传消融的构建体和方法。
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