公开(公告)号：US11739340B2

公开(公告)日：2023-08-29

申请号：US16938550

申请日：2020-07-24

申请人： Ceres, Inc.

发明人： Zhihong Cook ,  Yiwen Fang ,  Kenneth A. Feldmann ,  Edward Kiegle ,  Shing Kwok ,  Yu-Ping Lu ,  Leonard Medrano ,  Roger Pennell ,  Richard Schneeberger ,  Chuan-Yin Wu ,  Nestor Apuya ,  Jack K. Okamuro ,  Diane K. Jofuku ,  Jonathan Donson ,  David Van-Dinh Dang ,  Emilio Margolles-Clark ,  Nickolai Alexandrov ,  Tatiana Tatarinova ,  Noah Theiss ,  Danielle Grizard ,  Shawna Davis ,  Dennis Robles ,  Michael Portereiko

IPC分类号： C12N15/82

CPC分类号： C12N15/8222 ,  C12N15/823 ,  C12N15/8226 ,  C12N15/8227

摘要： The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.

公开(公告)号：US20230203516A1

公开(公告)日：2023-06-29

申请号：US17818060

申请日：2022-08-08

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： TIM FOX ,  WILLIAM JAMES GORDON-KAMM ,  RACHEL CAROL HUEGEL ,  KEITH S. LOWE ,  JON AARON TUCKER REINDERS ,  HUAXUN YE

IPC分类号： C12N15/82 ,  A01H1/08 ,  C12N9/22

CPC分类号： C12N15/823 ,  A01H1/08 ,  C12N9/22 ,  C12N2310/20

摘要： Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.

公开(公告)号：US09534228B2

公开(公告)日：2017-01-03

申请号：US13114656

申请日：2011-05-24

申请人： Roderick Scott

发明人： Roderick Scott

IPC分类号： C12N15/82 ,  A01H5/10 ,  C07K14/415

CPC分类号： C12N15/8261 ,  A01H5/10 ,  C07K14/415 ,  C12N15/823 ,  C12N15/8234 ,  Y02A40/146

摘要： This invention relates to methods for altering cell proliferation in a plant by modulating the expression of an endogenous plant gene whose expression product affects cell proliferation. These methods can be used to modify plant characteristics, such as seed size. In particular, the invention provides methods for modifying cell proliferation in the integuments and/or seed coats of a plant by inhibiting expression of endogenous gene encoding Auxin response factor 2 (ARF2/MNT) or an orthologue thereof, such that the number of cells in the integuments and/or seed coat is increased, resulting in increased seed size. The plant or plant propagating material can be transformed, e.g., with a nucleic acid construct, to increase or decrease the expression of endogenous genes or the orthologues thereof that affect cell proliferation. The invention also relates to modified plants and reproducible plant material produced by the methods, and nucleic acid constructs for use in such methods.

摘要翻译： 本发明涉及通过调节其表达产物影响细胞增殖的内源植物基因的表达来改变植物细胞增殖的方法。 这些方法可用于修改植物特性，如种子大小。 特别地，本发明提供了通过抑制编码生物素应答因子2（ARF2 / MNT）或其直向同源物的内源基因的表达来修饰植物的外皮和/或种皮中的细胞增殖的方法， 皮和/或种皮增加，导致种子大小增加。 植物或植物繁殖材料可以例如用核酸构建体进行转化，以增加或减少影响细胞增殖的内源基因或其直向同源物的表达。 本发明还涉及通过所述方法生产的经修饰的植物和可重复植物材料，以及用于这些方法的核酸构建体。

公开(公告)号：US20160017346A1

公开(公告)日：2016-01-21

申请号：US14772729

申请日：2014-03-12

申请人： E.I. DU PONT DE NEMOURS AND COMPANY

发明人： Zhongsen Li

IPC分类号： C12N15/82

CPC分类号： C12N15/823 ,  C07K14/415

摘要： The invention relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean predicted allergen Gly m Bd 28K peptide gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-specific manner in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.

摘要翻译： 本发明涉及大豆的基因表达调控序列，特别涉及大豆预测变应原Gly m Bd 28K肽基因及其片段的启动子及其在促进一种或多种异源核酸片段以组织特异性方式表达的用途 在植物中。 本发明还公开了组合物，多核苷酸构建体，转化的宿主细胞，转基因植物和含有重组构建体的启动子的种子，以及制备和使用该重组构建体的方法。

公开(公告)号：US20150376631A1

公开(公告)日：2015-12-31

申请号：US14772711

申请日：2014-03-12

申请人： E.I. DU PONT DE NEMOURS AND COMPANY

发明人： Zhongsen Li

IPC分类号： C12N15/82

CPC分类号： C12N15/823 ,  C12N15/8234

摘要： The invention relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean hypersensitive-induced response protein gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-specific manner in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.

摘要翻译： 本发明涉及大豆，特别是大豆过敏诱导的应答蛋白基因及其片段的启动子的基因表达调控序列及其在植物中以组织特异性方式促进一种或多种异源核酸片段的表达的用途 。 本发明还公开了组合物，多核苷酸构建体，转化的宿主细胞，转基因植物和含有重组构建体的启动子的种子，以及制备和使用该重组构建体的方法。

公开(公告)号：US20150128302A1

公开(公告)日：2015-05-07

申请号：US14539673

申请日：2014-11-12

申请人： PIONEER HI BRED INTERNATIONAL INC

发明人： Shane Abbitt

IPC分类号： C12N15/82 ,  C12N15/113

CPC分类号： C12N15/8216 ,  C12N15/113 ,  C12N15/8223 ,  C12N15/823

摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a constitutive regulatory element isolated from sorghum. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

摘要翻译： 本发明提供调节植物中异源核苷酸序列表达的组合物和方法。 组合物是从高粱分离的组成型调节元件的新型核苷酸序列。 提供了使用本文公开的调节序列在植物中表达异源核苷酸序列的方法。 该方法包括将植物细胞转化为包含可操作地连接到本发明的一个或多个调控序列的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。

公开(公告)号：US20130227742A1

公开(公告)日：2013-08-29

申请号：US13775341

申请日：2013-02-25

申请人： Pioneer Hi Bred International Inc

发明人： Shane E Abbitt

IPC分类号： C12N15/113

CPC分类号： C12N15/8216 ,  C12N15/113 ,  C12N15/8223 ,  C12N15/823

摘要： The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a constitutive regulatory element isolated from sorghum. A method for expressing a heterologous nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.

公开(公告)号：US08476493B2

公开(公告)日：2013-07-02

申请号：US13242193

申请日：2011-09-23

申请人： William H. Rottmann ,  Kim H. Norris-Caneda ,  Chunsheng Zhang

发明人： William H. Rottmann ,  Kim H. Norris-Caneda ,  Chunsheng Zhang

IPC分类号： C12N15/82 ,  C12N15/55 ,  C12N5/04 ,  C12N5/10 ,  C12N15/31 ,  A01H5/00 ,  A01H1/02

CPC分类号： C12N15/8289 ,  C07K14/415 ,  C12N9/22 ,  C12N15/8205 ,  C12N15/823 ,  C12N15/8231 ,  C12N15/8233 ,  C12N15/8234 ,  C12N15/8263 ,  C12N15/827 ,  C12N15/829

摘要： The present invention relates to the regulation of reproductive development, particularly to the genetic ablation of reproductive tissues in angiosperm and gymnosperm species. Reproductive-preferred promoters, regulatory elements, and cytotoxic nucleotide sequences are disclosed herein, as are constructs and methods for genetic ablation.

摘要翻译： 本发明涉及生殖发育的调节，特别涉及被子植物和裸子植物物种中生殖组织的遗传消融。 生殖优选的启动子，调节元件和细胞毒性核苷酸序列在本文中公开，以及用于遗传消融的构建体和方法。

公开(公告)号：US08389805B2

公开(公告)日：2013-03-05

申请号：US12103970

申请日：2008-04-16

申请人： Nestor Apuya ,  Shing Kwok ,  Nickolai Alexandrov ,  Tatiana Tatarinova ,  Yiwen Fang ,  Roger Pennell ,  Yu-Ping Lu ,  Leonard Medrano ,  Zhihong Cook ,  Kenneth A. Feldmann

发明人： Nestor Apuya ,  Shing Kwok ,  Nickolai Alexandrov ,  Tatiana Tatarinova ,  Yiwen Fang ,  Roger Pennell ,  Yu-Ping Lu ,  Leonard Medrano ,  Zhihong Cook ,  Kenneth A. Feldmann

IPC分类号： A01H1/00 ,  C12N15/63 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N15/8237 ,  C12N15/8225 ,  C12N15/8226 ,  C12N15/8227 ,  C12N15/8229 ,  C12N15/823 ,  C12N15/8233 ,  C12N15/8234 ,  Y10T436/143333

摘要： The present invention is directed to promoter sequences and promoter control elements, polynucleotide constructs comprising the promoters and control elements, and methods of identifying the promoters, control elements, or fragments thereof. The invention further relates to the use of the present promoters or promoter control elements to modulate transcript levels.

摘要翻译： 本发明涉及启动子序列和启动子控制元件，包含启动子和控制元件的多核苷酸构建体，以及鉴定启动子，控制元件或其片段的方法。 本发明还涉及本发明启动子或启动子控制元件调节转录水平的用途。

公开(公告)号：US08222484B2

公开(公告)日：2012-07-17

申请号：US12888469

申请日：2010-09-23

申请人： Olga Danilevskaya ,  Milo J Auckerman ,  Pedro Hermon ,  Evgueni Ananiev ,  David Mark Shirbroun ,  Michael G Muszynski

发明人： Olga Danilevskaya ,  Milo J Auckerman ,  Pedro Hermon ,  Evgueni Ananiev ,  David Mark Shirbroun ,  Michael G Muszynski

IPC分类号： C12N15/29 ,  C12N15/82 ,  C12N5/04 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N15/827 ,  C07K14/415 ,  C12N15/11 ,  C12N15/8201 ,  C12N15/8222 ,  C12N15/823 ,  C12N15/8241 ,  C12N2830/008

摘要： The invention provides isolated floral transition nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering floral transition in plants. The invention further provides regulatory elements, recombinant expression cassettes, host cells, and transgenic plants.

摘要翻译： 本发明提供了分离的花转移核酸及其编码的蛋白质。 本发明提供了关于改变植物花转变的方法和组合物。 本发明还提供调节元件，重组表达盒，宿主细胞和转基因植物。