公开(公告)号：US07553943B2

公开(公告)日：2009-06-30

申请号：US10578460

申请日：2004-11-08

Applicant: Darren James Ellis ,  Colin Lloyd Barnes ,  Harold Philip Swerdlow ,  Tom Brown

Inventor： Darren James Ellis ,  Colin Lloyd Barnes ,  Harold Philip Swerdlow ,  Tom Brown

IPC: C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C07H21/00 ,  C12Q1/6837 ,  C12Q2525/301 ,  C12Q2525/113

Abstract: The invention provides a hairpin polynucleotide, having a loop and a stem region, characterised in that a sulfur-based nucleophile is attached to an internal nucleotide in the hairpin through a linker to enable binding to a solid support.

Abstract translation: 本发明提供了具有环和茎区的发夹多核苷酸，其特征在于通过连接体将硫基亲核试剂连接到发夹中的内部核苷酸以使得能够结合固体支持物。

公开(公告)号：US06481261B1

公开(公告)日：2002-11-19

申请号：US09560748

申请日：2000-04-28

Applicant: Darren James Ellis

Inventor： Darren James Ellis

IPC: B21J1100

CPC classification number: B21D51/26 ,  B23Q7/06 ,  B23Q7/14

Abstract: There is described a feed mechanism for a feed table for translating sheet metal blanks from as automatic notching curling and beading machine to a bodymaker in the process of tin box manufacture. The mechanism comprises a pair of reciprocally drives longitudinal feed bars provided with spring loaded pawls in their upper surfaces which engage behind the edges of the blanks during a forward motion and slide beneath the blanks during a reverse motion. The difficulty of altering the lateral displacement of the respective feed bars to allow the table to transfer blanks of different sizes is overcome by using a telescopic fixing member which can be extended to an infinitesimal degree and then secured. In practice, both the longitudinal feed bars are secured to a reciprocally driven transfer table within the stationary feed table, and it is the connection of the feed bars to this table that is achieved with telescopic fixing members. Additionally, the feed bars may be disposed in guide means for both support and to ensure precisely linear back and forth motion parallel with the central axis of the table. In this manner reliable and trouble free transfer of blanks is effected.

Abstract translation: 描述了用于在锡盒制造过程中将钣金坯料从自动开槽卷曲和卷边机转移到主体的进给台的进给机构。 该机构包括一对往复驱动的纵向进给杆，在其上表面中设有弹簧加载的棘爪，其在向前运动期间接合在坯件的边缘之后，并且在反向运动期间在坯件下方滑动。 通过使用伸缩固定构件来克服各个进给杆的横向位移以允许工作台转移不同尺寸的坯料的难度，该可伸缩固定构件可以扩展到无穷小程度并且然后固定。 在实践中，两个纵向进给杆固定在固定进给台内的往复驱动的转台上，并且是通过伸缩式固定构件实现的进给杆与该工作台的连接。 另外，进给杆可以设置在用于支撑件的引导装置中并且确保与工作台的中心轴平行的精确的线性前后运动。 以这种方式可靠和无故障地转移坯料。

公开(公告)号：US20160115524A1

公开(公告)日：2016-04-28

申请号：US13989782

申请日：2011-11-28

Applicant: Darren James Ellis ,  Geir Fonnum ,  Nini Hofslokken Kjus

Inventor： Darren James Ellis ,  Geir Fonnum ,  Nini Hofslokken Kjus

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support whilst maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.

Abstract translation: 一种用于处理核酸的方法，其中所述核酸暴露于水性培养基，所述水性培养基包含足够比例的多元醇，用于所述核酸的至少一部分进入或保留在超溶液相中。 因此，多元醇可以用于将溶液中的核酸与固体支持物结合，或者将固体支持物上的核酸同时保持在载体上。 多元醇可以是例如C 2 -C 10链烷二醇。

公开(公告)号：US06434997B1

公开(公告)日：2002-08-20

申请号：US09684078

申请日：2000-10-07

Applicant: Darren James Ellis

Inventor： Darren James Ellis

IPC: B21D1702

CPC classification number: B21D19/12 ,  B21D17/02

Abstract: A method and apparatus (2) for providing at least a bead (4) on a side wall portion (10) of a metal container (8). The apparatus includes a mandrel (16) over which side wall portions (10) of the container are disposed. The mandrel (16) has reaction members (20) with reaction surfaces for supporting the side wall portion internally and a plurality of forming members (18) surrounding the mandrel having forming surfaces being substantially parallel with the reaction surfaces but separated therefrom to define a gap between the surfaces which receives the side wall portion (10). The reaction members (20) and forming members (18) are capable of displacement towards and away from one another such that the gap between the reaction surfaces and forming surfaces can effectively expand and contract. The reaction surfaces and forming surfaces are provided with complementary formations thereon to deform the side wall portion therebetween and form a bead (4) therein. The reaction members (20) and the forming members (18) are moved back and forth by virtue of cam means particular to each reaction member and forming member and the cam means are provided on an actuating member (26) having a linear reciprocal motion parallel with the axis of the mandrel (16). A curl (6) can also be formed on the container using curl forming means (44) which are also provided on the actuating member (26).

Abstract translation: 一种用于在金属容器（8）的侧壁部分（10）上提供至少一个珠（4）的方法和装置（2）。 该设备包括心轴（16），容器的侧壁部分（10）设置在该心轴上。 心轴（16）具有反作用构件（20），其具有用于在内部支撑侧壁部分的反作用表面，并且围绕心轴的多个成形构件（18）具有与反作用表面基本平行但形成间隔开的间隙 在接收侧壁部分（10）的表面之间。 反作用构件（20）和成形构件（18）能够朝向和远离彼此移动，使得反作用表面和成形表面之间的间隙能够有效地膨胀和收缩。 反应表面和成型表面在其上设置互补结构，以使其侧壁部分变形，并在其中形成凸缘（4）。 反作用构件（20）和成形构件（18）由于每个反作用构件和成形构件特别的凸轮装置而前后移动，并且凸轮装置设置在具有线性往复运动平行的致动构件（26）上 与心轴（16）的轴线。 也可以使用也设置在致动构件（26）上的卷曲形成装置（44）在容器上形成卷曲（6）。