公开(公告)号：US20230294086A1

公开(公告)日：2023-09-21

申请号：US18016753

申请日：2021-07-29

Applicant: Colorado State University Research Foundation

Inventor： Charles S. Henry ,  Brian J. Geiss ,  David S. Dandy ,  Cody Carrell ,  Jeremy Link ,  Isabelle Samper ,  Ana Sanchez-Cano ,  Ilhoon Jang ,  Zachary Call

IPC: B01L3/00 ,  G01N33/543

CPC classification number: B01L3/5023 ,  G01N33/54388 ,  B01L3/50273 ,  B01L2200/16 ,  B01L2400/0406 ,  B01L2300/069 ,  B01L2300/088

Abstract: An assay device includes a colorimetric testing assembly including a detection area, a fluid inlet, and a microfluidic network including a first path extending to the detection area and a second path extending to the detection area. When a fluid (e.g., a buffer fluid or a combined buffer and sample solution) is provided to the fluid inlet, a first portion of the fluid rehydrates a first dried reagent (e.g., a dried enzyme label) disposed along the first path to produce a first rehydrated reagent and a second portion of the fluid rehydrates a second dried reagent (e.g., a dried substrate) to produce a second rehydrated reagent. The first rehydrated reagent and the second rehydrated reagent are then sequentially delivered to the detection area by capillary-driven flow to perform the assay.

公开(公告)号：US11291997B2

公开(公告)日：2022-04-05

申请号：US16530638

申请日：2019-08-02

Applicant: Colorado State University Research Foundation ,  The United States of America, as represented by the Secretary of Agriculture

Inventor： Charles S. Henry ,  Rachel Feeny ,  Alan B. Franklin ,  Cody Carrell

IPC: B01L3/00

Abstract: A system for and methods of analyzing a test sample through the use of a rotary apparatus that includes a microfluidic paper-based apparatus (mPAD). The apparatus includes two or more layers that are rotatable with respect to one another. A middle layer may comprise a microfluidic apparatus having one or more reagent channels. Each of the reagent channels may include reagent dried on the surface of the channel, and, together with an absorption pad, may be aligned vertically with a sample chamber. Male and female engagement surfaces on each of the middle layer, the top layer, and the bottom layer interlock to secure each layers in vertical alignment so that fluid flows through the apparatus to contact a test sample with a reagent and facilitate detection of a target analyte in the test sample in the sample chamber.