公开(公告)号：US20210062168A1

公开(公告)日：2021-03-04

申请号：US17005913

申请日：2020-08-28

Applicant: Codexis, Inc. ,  Societe des Produits Nestle S.A.

Inventor： Chinping Chng ,  William Casey Hallows ,  Judy Victoria Antonio Viduya ,  Nikki Dellas ,  Stephanie Sue Galanie ,  Kristen Jean Vallieu

IPC: C12N9/20 ,  A61K38/46 ,  A61P5/48 ,  A61K9/48

Abstract: The present invention provides engineered lipase polypeptides and compositions thereof. The engineered lipase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH 7) conditions. The invention also relates to the use of the compositions comprising the engineered lipase polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered lipase polypeptides, as well as methods for making the engineered polynucleotides and lipase polypeptides.

公开(公告)号：US11987823B2

公开(公告)日：2024-05-21

申请号：US17005913

申请日：2020-08-28

Applicant: Societe des Produits Nestle S.A.

Inventor： Chinping Chng ,  William Casey Hallows ,  Judy Victoria Antonio Viduya ,  Nikki Dellas ,  Stephanie Sue Galanie ,  Kristen Jean Vallieu

IPC: C12N9/20 ,  A61K9/48 ,  A61K38/46 ,  A61P5/48

CPC classification number: C12N9/20 ,  A61K9/4883 ,  A61K38/465 ,  A61P5/48

Abstract: The present invention provides engineered lipase polypeptides and compositions thereof. The engineered lipase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH 7) conditions. The invention also relates to the use of the compositions comprising the engineered lipase polypeptides for therapeutic and/or nutritional purposes. The present invention also provides polynucleotides encoding the engineered lipase polypeptides, as well as methods for making the engineered polynucleotides and lipase polypeptides.

公开(公告)号：US20240263156A1

公开(公告)日：2024-08-08

申请号：US18630219

申请日：2024-04-09

Applicant: SOCIETE DES PRODUITS NESTLE S.A.

Inventor： Chinping Chng ,  William Casey Hallows ,  Judy Victoria Antonio Viduya ,  Nikki Dellas ,  Stephanie Sue Galanie ,  Kristen Jean Vallieu

IPC: C12N9/20 ,  A61K9/48 ,  A61K38/46 ,  A61P5/48

CPC classification number: C12N9/20 ,  A61K9/4883 ,  A61K38/465 ,  A61P5/48

Abstract: Engineered lipase polypeptides have been optimized to provide improved thermostability, protease stability, and stability under a range of pH conditions, including acidic (pH 7) conditions. Compositions containing the engineered lipase polypeptides can be used for therapeutic and/or nutritional purposes. Polynucleotides encoding the engineered lipase polypeptides are also provided, as well as methods for making the engineered polynucleotides and lipase polypeptides.

公开(公告)号：US11421253B2

公开(公告)日：2022-08-23

申请号：US17148834

申请日：2021-01-14

Applicant: Codexis, Inc.

Inventor： Gjalt W. Huisman ,  Joyce Liu ,  Nikki Dellas ,  Chinping Chng ,  William Casey Hallows

IPC: C12N9/88 ,  C12P7/42 ,  C12P13/22 ,  C12N15/63 ,  A61K9/00 ,  A61K31/122

Abstract: The present invention provides engineered tyrosine ammonia-lyase (TAL) polypeptides and compositions thereof. In some embodiments, the engineered TAL polypeptides have been optimized to provide enhanced catalytic activity and enhanced acid stability, while reducing sensitivity to proteolysis and increasing tolerance to acidic pH levels. The invention also provides methods for utilization of the compositions comprising the engineered TAL polypeptides for therapeutic and industrial purposes.

公开(公告)号：US20210189365A1

公开(公告)日：2021-06-24

申请号：US17126647

申请日：2020-12-18

Applicant: Codexis, Inc.

Inventor： William Casey Hallows ,  Rachel Cathleen Botham ,  Yu Zhu ,  Chinping Chng ,  Nikki Dellas ,  Gjalt W. Huisman ,  Moulay Hicham Alaoui Ismaili ,  David William Homan ,  Adam P. Silverman ,  Jonathan Vroom ,  Jessica P. Lao

IPC: C12N9/34

Abstract: The present invention provides engineered acid alpha-glucosidase (GAA) polypeptides and compositions thereof. In some embodiments, the engineered GAA polypeptides have been optimized to provide increased expression, stability at neutral pH, and activity in cell lysates. The invention also provides methods for utilization of the compositions comprising the engineered GAA polypeptides for therapeutic and other purposes.

公开(公告)号：US20180230448A1

公开(公告)日：2018-08-16

申请号：US15894446

申请日：2018-02-12

Applicant: Codexis, Inc.

Inventor： Chinping Chng ,  William Casey Hallows ,  Nicholas J. Agard ,  Oscar Alvizo ,  Nikki Dellas ,  Gjalt W. Huisman ,  John Joseph Nicols

IPC: C12N9/88 ,  A61K9/00

CPC classification number: C12N9/88 ,  A61K9/0019 ,  A61K9/0053 ,  A61K38/00 ,  A61K48/00 ,  C12Y403/01024

Abstract: The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activity, as well as reduced sensitivity to proteolysis and increased tolerance to storage at elevated temperatures. In some embodiments, the engineered PAL polypeptides contain fewer phenylalanine residues than wild-type PAL polypeptides. The present invention also provides methods for the use of the compositions comprising the engineered PAL polypeptides for therapeutic and industrial purposes.

公开(公告)号：US20230295598A1

公开(公告)日：2023-09-21

申请号：US18154388

申请日：2023-01-13

Applicant: Codexis, Inc.

Inventor： Chinping Chng ,  William Casey Hallows ,  Nicholas J. Agard ,  Oscar Alvizo ,  Nikki Dellas ,  Gjalt W. Huisman ,  John Joseph Nicols

IPC: C12N9/88 ,  C12N15/00

CPC classification number: C12N9/88 ,  C12Y403/01024 ,  C12N15/00 ,  A61K48/00

Abstract: The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activity, as well as reduced sensitivity to proteolysis and increased tolerance to storage at elevated temperatures. In some embodiments, the engineered PAL polypeptides contain fewer phenylalanine residues than wild-type PAL polypeptides. The present invention also provides methods for the use of the compositions comprising the engineered PAL polypeptides for therapeutic and industrial purposes.

公开(公告)号：US10995329B2

公开(公告)日：2021-05-04

申请号：US15894446

申请日：2018-02-12

Applicant: Codexis, Inc.

Inventor： Chinping Chng ,  William Casey Hallows ,  Nicholas J. Agard ,  Oscar Alvizo ,  Nikki Dellas ,  Gjalt W. Huisman ,  John Joseph Nicols

IPC: C12N9/88 ,  C12N15/00 ,  A61K48/00 ,  A61K38/00 ,  A61K9/00

Abstract: The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activity, as well as reduced sensitivity to proteolysis and increased tolerance to storage at elevated temperatures. In some embodiments, the engineered PAL polypeptides contain fewer phenylalanine residues than wild-type PAL polypeptides. The present invention also provides methods for the use of the compositions comprising the engineered PAL polypeptides for therapeutic and industrial purposes.

公开(公告)号：US20220356458A1

公开(公告)日：2022-11-10

申请号：US17814371

申请日：2022-07-22

Applicant: Codexis, Inc.

Inventor： William Casey Hallows ,  Kristen Jean Vallieu ,  Nikki Dellas ,  Yu Zhu ,  Judy Victoria Antonio Viduya ,  Chinping Chng ,  Antoinette Sero ,  Gjalt W. Huisman ,  Rachel Cathleen Botham ,  Moulay Hicham Alaoui Ismaili

IPC: C12N9/40 ,  C12N15/52 ,  C12N15/85

Abstract: The present invention provides engineered human alpha-galactosidase polypeptides and compositions thereof. The engineered human alpha-galactosidase polypeptides have been optimized to provide improved thermostability, serum stability, improved cellular uptake, stability under both acidic (pH 7) conditions, reduced immunogenicity, and improved globotriaosylceramide removal from cells. The invention also relates to the use of the compositions comprising the engineered human alpha-galactosidase polypeptides for therapeutic purposes.

公开(公告)号：US20210222145A1

公开(公告)日：2021-07-22

申请号：US17219494

申请日：2021-03-31

Applicant: Codexis, Inc.

Inventor： Chinping Chng ,  William Casey Hallows ,  Nicholas J. Agard ,  Oscar Alvizo ,  Nikki Dellas ,  Gjalt W. Huisman ,  John Joseph Nicols

IPC: C12N9/88 ,  C12N15/00

Abstract: The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activity, as well as reduced sensitivity to proteolysis and increased tolerance to storage at elevated temperatures. In some embodiments, the engineered PAL polypeptides contain fewer phenylalanine residues than wild-type PAL polypeptides. The present invention also provides methods for the use of the compositions comprising the engineered PAL polypeptides for therapeutic and industrial purposes.