公开(公告)号：US07767441B2

公开(公告)日：2010-08-03

申请号：US12255044

申请日：2008-10-21

申请人： Chung-Fan Chiou ,  Cheng-Wei Chu ,  Shang-Chia Chang ,  Yu-Tang Li ,  Chao-Chi Pan ,  Bin-Cheng Yao

发明人： Chung-Fan Chiou ,  Cheng-Wei Chu ,  Shang-Chia Chang ,  Yu-Tang Li ,  Chao-Chi Pan ,  Bin-Cheng Yao

IPC分类号： C12M3/00

CPC分类号： C12Q1/6869 ,  C12Q1/6804 ,  C12Q1/6874 ,  G01N21/6454 ,  C12Q2565/607 ,  C12Q2565/518 ,  C12Q2535/101

摘要： A bioassay system is disclosed. The bioassay system may include a plurality of optical detection apparatuses, each of which includes a substrate having a light detector, and a linker site formed over the light detector, the linker site being treated to affix the biomolecule to the linker site. The linker site is proximate to the light detector and is spaced apart from the light detector by a distance of less than or equal to 100 micrometers. The light detector collects light emitted from the biomolecule within a solid angle of greater than or equal to 0.8 SI steridian. The optical detection apparatus may further include an excitation light source formed over the substrate so as to provide a light source for exciting a fluorophore attached to the biomolecule.

摘要翻译： 公开了生物测定系统。 生物测定系统可以包括多个光学检测装置，每个光学检测装置包括具有光检测器的基板和在光检测器上形成的连接部位，所述连接部位被处理以将生物分子固定到连接部位。 接头位置靠近光检测器，并且与光检测器间隔开小于或等于100微米的距离。 光检测器以大于或等于0.8SI steridian的立体角收集从生物分子发射的光。 光学检测装置还可以包括形成在衬底上的激发光源，以便提供用于激发与生物分子相连的荧光团的光源。

公开(公告)号：US20110300534A1

公开(公告)日：2011-12-08

申请号：US13117565

申请日：2011-05-27

申请人： Chung-Fan Chiou ,  Chao-Chi Pan ,  Jenn-Yeh Fann ,  Shang-Chia Chang ,  Tsu-Ju Fu

发明人： Chung-Fan Chiou ,  Chao-Chi Pan ,  Jenn-Yeh Fann ,  Shang-Chia Chang ,  Tsu-Ju Fu

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C07H21/00 ,  C07H21/04 ,  C12Q1/6869 ,  C12Q2525/117 ,  C12Q2525/119 ,  C12Q2565/101

摘要： Embodiments relate to methods of sequencing nucleic acids. Embodiments encompass the use of nucleotide analogs and a nucleic acid polymerase enzyme or enzyme complex comprising proofreading activity. The nucleotide analogs may become incorporated into a replicating strand and induce the proofreading activity of the polymerizing enzyme, thereby prolonging the duration of a signal associated with nucleotide incorporation, resulting in more observable sequencing events and increasing the accuracy of nucleic acid sequencing.

摘要翻译： 实施方案涉及核酸测序方法。 实施方案包括使用核苷酸类似物和包含校正活性的核酸聚合酶或酶复合物。 核苷酸类似物可以并入复制链中并诱导聚合酶的校正活性，从而延长与核苷酸掺入相关的信号的持续时间，导致更可观测的测序事件并增加核酸测序的准确性。

公开(公告)号：US09670243B2

公开(公告)日：2017-06-06

申请号：US13117565

申请日：2011-05-27

申请人： Chung-Fan Chiou ,  Chao-Chi Pan ,  Jenn-Yeh Fann ,  Shang-Chia Chang ,  Tsu-Ju Fu

发明人： Chung-Fan Chiou ,  Chao-Chi Pan ,  Jenn-Yeh Fann ,  Shang-Chia Chang ,  Tsu-Ju Fu

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04 ,  C07H21/02 ,  C07H19/04

CPC分类号： C07H21/00 ,  C07H21/04 ,  C12Q1/6869 ,  C12Q2525/117 ,  C12Q2525/119 ,  C12Q2565/101

摘要： Embodiments relate to methods of sequencing nucleic acids. Embodiments encompass the use of nucleotide analogs and a nucleic acid polymerase enzyme or enzyme complex comprising proofreading activity. The nucleotide analogs may become incorporated into a replicating strand and induce the proofreading activity of the polymerizing enzyme, thereby prolonging the duration of a signal associated with nucleotide incorporation, resulting in more observable sequencing events and increasing the accuracy of nucleic acid sequencing.