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1.发明授权Aldolase catalyzed stereoselective synthesis of arabinohexulose, xyloheptulose, threohexulose and xylohexulose 失效醛缩酶催化立体选择性合成阿拉伯己离酮糖，木瓜庚酮糖，苏洛木糖和己基己糖
公开(公告)号：US5576426A
公开(公告)日：1996-11-19
申请号：US303289
申请日：1994-09-08
摘要： The present invention relates to aldolase catalyzed stereo-selective synthesis of sugars and compositions of matter comprising arabinohexulose, xyloheptulose, threohexulose, and xylohexulose.
摘要翻译： 本发明涉及醛缩酶催化的立体选择性合成糖和包含阿拉伯己离酮糖，木瓜庚酮糖，苏木素和木糖己糖的物质组合物。
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2.发明授权Fructose 1,6-diphosphate aldolase catalyzed steroselective synthesis of sugars 失效果糖1,6-二磷酸醛缩酶催化立体选择性合成糖
公开(公告)号：US5143831A
公开(公告)日：1992-09-01
申请号：US238756
申请日：1988-08-30
摘要： A method for preparing optically pure N-containing sugars is disclosed wherein an optically impure chiral aldehyde acceptor is enzymatically condensed with dihydroxyacetone phosphate or dihydroxyacetone with a catalytic amount of inorganic arsenate in the presence of an aldolase to form an N-containing sugar.
摘要翻译： 公开了一种用于制备光学纯的含N糖的方法，其中光学上不纯的手性醛受体在醛缩酶存在下用催化量的无机砷酸盐与二羟基丙酮磷酸酯或二羟基丙酮进行酶学缩合以形成含N的糖。
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公开(公告)号：US5565585A
公开(公告)日：1996-10-15
申请号：US225225
申请日：1994-04-08
申请人： Chi-Huey Wong , Richard L. Pederson , Yi-Fong Wang
发明人： Chi-Huey Wong , Richard L. Pederson , Yi-Fong Wang
IPC分类号： C07C45/89 , C07C247/04 , C07F9/02 , C07C211/00
CPC分类号： C07C247/04 , C07C45/89
摘要： A new and practical method for synthesizing heterocyclic polyhydroxylated alkaloids using enzymatic aldol condensation and catalytic intramolecular reductive amination is disclosed.
摘要翻译： 公开了一种使用酶促醛缩醇缩合和催化分子内还原胺化合成杂环多羟基化生物碱的新型实用方法。
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公开(公告)号：US5329025A
公开(公告)日：1994-07-12
申请号：US862157
申请日：1992-04-02
申请人： Chi-Huey Wong , Richard L. Pederson , Yi-Fong Wang
发明人： Chi-Huey Wong , Richard L. Pederson , Yi-Fong Wang
IPC分类号： C07C45/89 , C07C247/04 , C07C247/02 , C07C205/05 , C07C207/00 , C07C247/00
CPC分类号： C07C247/04 , C07C45/89
摘要： A new and practical method for synthesizing heterocyclic polyhydroxylated alkaloids using enzymatic aldol condensation and catalytic intramolecular reductive amination is disclosed.
摘要翻译： 公开了一种使用酶促醛缩醇缩合和催化分子内还原胺化合成杂环多羟基化生物碱的新型实用方法。
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公开(公告)号：US09816981B2
公开(公告)日：2017-11-14
申请号：US13159339
申请日：2011-06-13
申请人： Masaaki Sawa , Chi-Huey Wong , Tsui-Ling Hsu , Sarah Hanson
发明人： Masaaki Sawa , Chi-Huey Wong , Tsui-Ling Hsu , Sarah Hanson
IPC分类号： C07H15/04 , C07H19/10 , G01N33/50 , G01N33/533
CPC分类号： G01N33/5005 , G01N33/5008 , G01N33/533 , G01N2400/00
摘要： Methods for metabolic oligosaccharide engineering that incorporates derivatized alkyne-bearing sugar analogs as “tags” into cellular glycoconjugates are disclosed. Alkynyl derivatized Fuc and alkynyl derivatized ManNAc sugars are incorporated into cellular glycoconjugates. Chemical probes comprising an azide group and a visual or fluorogenic probe and used to label alkyne-derivatized sugar-tagged glycoconjugates are disclosed. Chemical probes bind covalently to the alkynyl group by Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition and are visualized at the cell surface, intracellularly, or in a cellular extract. The labeled glycoconjugate is capable of detection by flow cytometry, SDS-PAGE, Western blot, ELISA, confocal microscopy, and mass spectrometry.
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公开(公告)号：US08916540B2
公开(公告)日：2014-12-23
申请号：US12354717
申请日：2009-01-15
申请人： Chi-Huey Wong , Ting-Jen Cheng , Che Alex Ma , Wei-Chieh Cheng
发明人： Chi-Huey Wong , Ting-Jen Cheng , Che Alex Ma , Wei-Chieh Cheng
IPC分类号： C07K14/195 , C07K14/26 , C07K14/315 , C07K14/25 , C07K14/235 , C07K14/32 , C07K14/31 , C07K14/21 , C07K14/33 , C07K14/255 , C07K14/22 , C07K14/265 , G01N33/573 , A61K31/19 , A61K31/715 , A61K31/427 , A61K31/365
CPC分类号： A61K31/702 , A61K31/19 , A61K31/365 , A61K31/427 , A61K31/715 , C07K14/195 , C07K14/21 , C07K14/22 , C07K14/235 , C07K14/25 , C07K14/255 , C07K14/26 , C07K14/265 , C07K14/31 , C07K14/315 , C07K14/3156 , C07K14/32 , C07K14/33 , G01N33/573 , G01N2333/91091 , Y02A50/473 , Y02A50/475 , Y02A50/481
摘要： Moenomycin inhibits bacterial growth by clocking the transglycosylase activity of class A penicillin-binding proteins (PBPs), which are key enzymes in bacterial cell wall synthesis. The binding affinities of moenomycin A with various truncated PBPs were compared showing that the transmembrane domain is important for moenomycin binding. Full-length class-A PBPs from 16 bacterial species were produced, and their binding activities showed a correlation with the antimicrobial activity of moenomycin against Enterococcus faecalis and Staphylococcus aureus. Moreover, a fluorescence anisotropy-based high-throughput assay was developed and used successfully for identification of transglycosylase inhibitors.
摘要翻译： 霉菌素通过计时细菌细胞壁合成中的关键酶A类青霉素结合蛋白（PBP）的转糖苷酶活性抑制细菌生长。 比较了霉酚霉素A与各种截短的PBP的结合亲和力，结果表明跨膜结构域对于霉酚霉素结合是重要的。 产生了16种细菌种类的全长class-A PBPs，其结合活性与霉酚霉素对粪肠球菌和金黄色葡萄球菌的抗菌活性呈显着相关性。 此外，开发了基于荧光各向异性的高通量测定，并成功地用于鉴定转糖基酶抑制剂。
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7.发明授权Quantitative analysis of carbohydrate-protein interactions using glycan microarrays: determination of surface and solution dissociation constants 有权使用聚糖微阵列对碳水化合物 - 蛋白质相互作用的定量分析：测定表面和溶液解离常数公开(公告)号：US08906832B2
公开(公告)日：2014-12-09
申请号：US12423733
申请日：2009-04-14
申请人： Chi-Huey Wong , Pi-Hui Liang
发明人： Chi-Huey Wong , Pi-Hui Liang
CPC分类号： G01N33/5308 , B01J19/0046 , C40B30/04 , C40B40/12 , G01N2400/00 , G01N2400/10
摘要： A method, system and device to identify, study and/or mimic carbohydrate-protein interactions on cell surfaces and in solution measured by a glycan microarray. In some instances the method, system and device uses very small quantities of carbohydrate as low as attomol. In some instances the system, method and device is high-throughput. The small quantity sensitivity may allow for close placement of carbohydrate array members wherein due to close proximity multivalent interactions with proteins may be identified.
摘要翻译： 鉴定，研究和/或模拟细胞表面和聚糖微阵列测量溶液中碳水化合物 - 蛋白质相互作用的方法，系统和装置。 在某些情况下，该方法，系统和装置使用非常少量的碳水化合物，与阿托莫尔一样低。 在某些情况下，系统，方法和设备是高吞吐量的。 小量的灵敏度可以允许碳水化合物阵列成员的紧密放置，其中由于与蛋白质的近似多价相互作用可以被鉴定。
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公开(公告)号：US08481571B2
公开(公告)日：2013-07-09
申请号：US11121314
申请日：2005-05-03
申请人： Chi-Huey Wong , Chung-Yi Wu , Jia-Tsrong Jan
发明人： Chi-Huey Wong , Chung-Yi Wu , Jia-Tsrong Jan
IPC分类号： A61K31/44 , A61K31/40 , A61K31/165 , A61K31/325
CPC分类号： A61K31/165 , A61K31/325 , A61K31/40 , A61K31/44
摘要： A method of treating coronavirus infection. The method includes administering to a subject suffering from or being at risk of suffering from such infection an effective amount of a compound of formula (I). Each variable in this formula is defined in the specification.
摘要翻译： 治疗冠状病毒感染的方法。 该方法包括给患有或有风险患有此类感染的受试者施用有效量的式（I）化合物。 该公式中的每个变量在规范中定义。
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9.发明授权Five-membered iminocyclitol derivatives as selective and potent glycosidase inhibitors: new structures for antivirals and osteoarthritis therapeutics 有权五元亚氨基环醇衍生物作为选择性和有效的糖苷酶抑制剂：用于抗病毒药物和骨关节炎治疗剂的新结构公开(公告)号：US08273788B2
公开(公告)日：2012-09-25
申请号：US13033692
申请日：2011-02-24
申请人： Pi-Hui Liang , Yi-Ling Lin , Chi-Huey Wong
发明人： Pi-Hui Liang , Yi-Ling Lin , Chi-Huey Wong
IPC分类号： C07D207/00 , A61K31/40
CPC分类号： A61K31/4015 , Y02A50/385 , Y02A50/389
摘要： Novel 5-membered iminocyclitol derivatives were found to be a potent and selective inhibitors of the glycoprotein processing α- and β-glucosidase which were further found to be active antiviral agents against Japanese encephalitis virus, dengue virus serotype 2 (DEN-2), human SARS coronavirus and human β-hexosaminidase, a new target for development of osteoarthritis therapeutics.
摘要翻译： 发现新的5元亚氨基环醇衍生物是糖蛋白加工α-和β-葡萄糖苷酶的有效和选择性抑制剂，其进一步被发现是针对日本脑炎病毒，登革热病毒血清型2（DEN-2）的活性抗病毒剂， 人类SARS冠状病毒和人类 - 糖尿病病毒，是骨关节炎治疗发展的新靶点。
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10.发明申请GLYCOPROTEOMIC PROBES FOR FLUORESCENT IMAGING OF FUCOSYLATED GLYCANS IN VIVO 有权血液中富含荧光成像的GLYCOPROTEOMIC PROBES公开(公告)号：US20110257376A1
公开(公告)日：2011-10-20
申请号：US13053192
申请日：2011-03-21
申请人： Chi-Huey Wong , Tsui-Ling Hsu , Sarah R. Hanson , Masaaki Sawa
发明人： Chi-Huey Wong , Tsui-Ling Hsu , Sarah R. Hanson , Masaaki Sawa
IPC分类号： C07K14/47 , C07H19/207
CPC分类号： G01N33/582 , G01N1/30 , G01N2400/00
摘要： Methods are provided for labeling cellular glycans bearing azide groups via fluorescent labeling comprising Cu(I)-catalyzed [3+2] cycloaddition of a probe comprising alkynyl group. Generation of fluorescent probes from a nonfluorescent precursor, 4-ethynyl-N-ethyl-1,8-naphthalimide, by Cu(I)-catalyzed [3+2] cycloaddition of the alkyne group of the probe to an azido-modified sugar are provided. Incorporation of azido-containing fucose analog into glycoconjugates via the fucose salvage pathway are disclosed. Fluorescent visualization of fucosylated cells by flow cytometry of cells treated with 6-azidofucose labeled with click-activated fluorogenic probe or biotinylated alkyne is disclosed. Visualization of intracellular location of fucosylated glycoconjugates by fluorescence microscopy are disclosed.
摘要翻译： 提供了通过含有包含炔基的探针的Cu（I）催化的[3 + 2]环加成的荧光标记来标记携带叠氮基的细胞聚糖的方法。 通过Cu（I）催化的[3 + 2]环加成的探针的炔基与叠氮改性的糖，从非荧光前体4-乙炔基-N-乙基-1,8-萘二甲酰亚胺生成荧光探针是 提供。 公开了通过岩藻糖补救途径将叠氮基岩藻糖类似物掺入糖缀合物。 公开了通过用用点击激活的荧光探针或生物素化的炔标记的6-叠氮基聚糖处理的细胞的流式细胞术的岩藻糖基化细胞的荧光可视化。 公开了通过荧光显微镜观察岩藻糖基化糖缀合物的细胞内位置。
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