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公开(公告)号：US20170350763A1
公开(公告)日：2017-12-07
申请号：US15524119
申请日：2015-11-05
Applicant: Carl Zeiss Microscopy GmbH
Inventor： David SHAFER , Tiemo ANHUT , Matthias WALD
CPC classification number: G01J3/4406 , G01J3/0208 , G01J3/021 , G01J3/14 , G01J3/18 , G01J3/36 , G01N21/6458 , G01N2021/6421 , G01N2201/068 , G02B21/0064 , G02B21/0076 , G02B21/16 , G02B21/361 , G02B27/0025 , G02B27/1013 , G02B27/126
Abstract: A detection device (113) for a microscope comprises a dispersive element (211) in the beam path (290) of light and a selection element (212). The selection element (212) separates a beam path (291) of a spectral portion of the light from the beam path (290) of the light. The detector device (113) furthermore comprises a focusing optical unit (213) configured to focus the beam path (291) of the spectral portion of the light onto a sensor (214). By way of example, the microscope may be a confocal microscope.
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公开(公告)号：US20190227290A1
公开(公告)日：2019-07-25
申请号：US16182422
申请日：2018-11-06
Applicant: Carl Zeiss Microscopy GmbH , Carl Zeiss AG
Inventor： Daniel SCHWEDT , Matthias WALD , Tiemo ANHUT
IPC: G02B21/00
CPC classification number: G02B21/0032 , G02B21/002 , G02B21/004 , G02B21/0052 , G02B21/0076 , G02B21/008
Abstract: A functionally integrated laser scanning microscope for scanning a sample with laser illumination, selectably in a confocal, line or wide-field operating mode, comprising a laser light source, an illumination and detection beam path, a detection device and at least one objective, wherein the illumination and detection beam path has optical means for the configuration of the laser illumination, at least one scanner for scanning the sample with the laser illumination, and a beam splitter for separating illumination and detection light, and controllable optical elements for changing the beam guiding depending on the operating mode selected in each case.
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公开(公告)号：US20170293126A1
公开(公告)日：2017-10-12
申请号：US14645697
申请日：2015-03-12
Applicant: Carl Zeiss Microscopy GmbH
Inventor： Matthias WALD
IPC: G02B21/00
CPC classification number: G02B21/002 , G02B21/0032 , G02B21/06 , G02B27/10
Abstract: A laser-scanning microscope having an illumination-beam path and a detection-beam path and a microscope objective. A component for generating a plurality of scanning beams from at least one illumination beam is located in the illumination-beam path. A wedge-shaped, light-transmitting first component part provided in the illumination beam path generates spatially offset partial beams, the scanning beams being generated at the first component part by multiple reflections at an at least partially partially-reflecting surface. The microscope has a one-dimensional scanner for moving the scanning beams over a sample in the illumination beam path. The scanning beams have at least partially relative to one another a non-zero angle upstream of the objective in the illumination direction. The scanning beams can intersect at least partially in the objective pupil of the microscope objective. Additional compensation elements are provided for the scanning beams to compensate for a spectral dispersion and/or the beam direction.
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公开(公告)号：US20200183139A1
公开(公告)日：2020-06-11
申请号：US16641567
申请日：2018-08-23
Applicant: Carl Zeiss Microscopy GmbH
Inventor： Tiemo ANHUT , Matthias WALD , Daniel SCHWEDT , Beate BÖHME
Abstract: An optical assembly for scanning excitation radiation and/or manipulation radiation in a laser scanning microscope. The assembly an optical scanning unit as a first focusing device for providing a first pupil plane, a first beam deflecting device, which is made of a first scanner arranged in the first pupil plane, for scanning the excitation radiation and/or manipulation radiation in a first coordinate direction, and a second focusing device for generating a second pupil plane, which is optically conjugated to the first pupil plane. A second beam deflecting device is provided for deflecting the excitation radiation and/or manipulation radiation, said second deflecting device being arranged in the second pupil plane. A third focusing device is provided in order to generate a third pupil plane, optically conjugated to the first pupil plane. A third beam deflecting device is arranged in the third pupil plane in order to deflect the excitation radiation and/or manipulation radiation, and a variable beam deflecting means is provided in order to switch an optical beam path between a first beam path and a second beam path.
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5.发明申请公开(公告)号：US20180113292A1
公开(公告)日：2018-04-26
申请号：US15573429
申请日：2016-05-09
Applicant: Carl Zeiss Microscopy GmbH , Carl Zeiss AG
Inventor： Yauheni NOVIKAU , Thomas KALKBRENNER , Tiemo ANHUT , Daniel SCHWEDT , Matthias WALD
CPC classification number: G02B21/0076 , G01N21/6408 , G01N21/6458 , G02B21/006 , G02B21/008
Abstract: A method for evaluating signals of fluorescence scanning microscopy with simultaneous excitation and detection of fluorescence in different focal planes of a sample by means of confocal laser scanning microscopy. The invention evaluates signals of fluorescence scanning microscopy without the signal losses usually taking place with a confocal aperture, by coupling an illumination beam into a microscope observation beam path which images a measuring volume on a detector array arranged in the image plane, focusing the illumination beam which passes through a beam-forming phase mask for generating an elongated focus in the measuring volume, collecting and collimating fluorescent light generated in the measuring volume and routing it to diffractive optics which split the light beams into different diffraction orders and impress a different spherical phase on the light beams, imaging the different diffraction orders on detector regions of the detector array so that fluorescent light from focal planes at different depths of the measuring volume are associated with different diffraction orders, and associating the fluorescence signals on which crosstalk is superposed from different focal planes of the measuring volume with defined focal planes by means of correlation-based association based on distinguishable blinking behavior of fluorescing dyes.
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Patent Agency Ranking
公开(公告)号：US20210141205A1
公开(公告)日：2021-05-13
申请号：US16492902
申请日：2018-03-01
Applicant: Carl Zeiss Microscopy GmbH
Inventor： Ingo KLEPPE , Matthias WALD
Abstract: A microscopy method for three-dimensionally imaging an object, including imaging the object along a beam path into a first image on a first image plane. A first microlens array is arranged on the first image plane, and a second microlens array with the same pitch is arranged downstream of the first array. The two arrays laterally segment the first image and image same into a second image in which the segments are spaced apart and separated by gaps. On a pupil plane downstream of the microlens array, a phase mask is provided which generates a spot for each segment of the second image according to a pixel diffusion function. A detector detects the shape and structure of the spot, and a controller ascertains a lateral intensity distribution and depth specification from the shape and/or structure of the spot for each segment and generates a depth-resolved image of the object therefrom.
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公开(公告)号：US20190170995A1
公开(公告)日：2019-06-06
申请号：US16313645
申请日：2017-06-29
Applicant: Carl Zeiss Microscopy GmbH
Inventor： Dr. Jörg SIEBENMORGEN , Helmut LIPPERT , Thomas KALKBRENNER , Ingo KLEPPE , Ralf WOLLESCHENSKY , Artur DEGEN , Matthias WALD , Lars-Christian WITTIG , Michael GÖLLES , Wolfgang SINGER
Abstract: An arrangement for microscopy, having an illumination optical unit with an illumination objective for illuminating a specimen situated on a specimen carrier in a specimen region of a specimen plane via an illumination beam path. An optical axis of the illumination objective lies in a plane which includes an illumination angle that differs from zero with the normal of a specimen plane, in respect of which the specimen carrier is aligned, and the illumination is implemented in the plane. Further, a detection optical unit with a detection objective is located in a detection beam path. The optical axis of the detection objective includes a detection angle that differs from zero with the normal of the specimen plane. The illumination objective and/or the detection objective comprises an illumination correction element arranged in the beam path and/or a detection correction element. A meniscus lens is located between the specimen carrier and the illumination and detection objectives, said meniscus lens being arranged both in the illumination beam path and in the detection beam path and being configured to correct aberrations. The illumination correction element and/or the detection correction element is/are configured to correct remaining aberrations.
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公开(公告)号：US20210157117A1
公开(公告)日：2021-05-27
申请号：US16641516
申请日：2018-08-23
Applicant: Carl Zeiss Microscopy GmbH
Inventor： Tiemo ANHUT , Matthias WALD , Daniel SCHWEDT , Tobias KAUFHOLD
Abstract: An optical assembly for scanning excitation radiation and/or manipulation radiation in a laser scanning microscope, having an optical scanning unit for providing a first pupil plane, a first beam deflecting device, which is made of a first scanner arranged on the first pupil plane, for scanning the excitation radiation and/or manipulation radiation in a first coordinate direction, a first focusing device for generating a second pupil plane, which is optically conjugated to the first pupil plane, and a second beam deflecting device for deflecting the excitation radiation and/or manipulation radiation, said second deflecting device being arranged on the second pupil plane, a second focusing device in order to generate a third pupil plane, which is optically conjugated to the first pupil plane and the second pupil plane, a third beam deflecting device is arranged on the third pupil plane for deflecting the excitation radiation and/or manipulation radiation, and a variable beam deflecting means is provided between the first focusing device and the second pupil plane and the second pupil plane and the second focusing device in order to switch an optical beam path between a first beam path and a second beam path.
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公开(公告)号：US20170199362A1
公开(公告)日：2017-07-13
申请号：US15314063
申请日：2015-05-22
Applicant: Carl Zeiss Microscopy GmbH , Carl Zeiss AG
Inventor： Daniel SCHWEDT , Matthias WALD , Tiemo ANHUT
IPC: G02B21/00
CPC classification number: G02B21/0032 , G02B21/002 , G02B21/004 , G02B21/0052 , G02B21/0076 , G02B21/008
Abstract: A functionally integrated laser scanning microscope for scanning a sample with laser illumination, selectably in a confocal, line or wide-field operating mode, comprising a laser light source, an illumination and detection beam path, a detection device and at least one objective, wherein the illumination and detection beam path has optical means for the configuration of the laser illumination, at least one scanner for scanning the sample with the laser illumination, and a beam splitter for separating illumination and detection light, and controllable optical elements for changing the beam guiding depending on the operating mode selected in each case.
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公开(公告)号：US20160377850A1
公开(公告)日：2016-12-29
申请号：US15117849
申请日：2015-02-09
Applicant: CARL ZEISS MICROSCOPY GMBH , CARL ZEISS AG
Inventor： Dr. Tiemo ANHUT , Daniel SCHWEDT , Matthias WALD
Abstract: Scanning fluorescence microscopes with an observation beam path from a measurement volume to an image plane. A beam combiner is provided for coupling an illumination system and a diaphragm arranged in the image plane for slow composition of the image because of the sequential scanning and subject the sample to loading as a result of inefficient use of the excitation light. The microscope simultaneously detects fluorescence from different focal planes in each case quasi-confocally. The observation beam path between the beam combiner and the image plane has a first diffractive optics for splitting light beams into beam bundles along different orders of diffraction, imparting to the light beams a spherical phase that is different from the other orders of diffraction. A second diffractive optics is provided for the compensation of chromatic aberrations of the split beam bundles, and a collecting optics is provided for focusing split beam bundles into the image plane.
Abstract translation: 用从测量体积到图像平面的观察光束路径扫描荧光显微镜。 提供了一种光束组合器，用于耦合照明系统和布置在图像平面中的光阑，用于由于顺序扫描而慢速组合图像，并且由于激发光的低效使用而使样本加载。 显微镜同时检测来自不同焦平面的荧光，在每种情况下准共聚。 光束组合器和像平面之间的观察光束路径具有第一衍射光学器件，用于沿着不同的衍射级将光束分成束束，从而将光束赋予不同于其它衍射级的球形相。 提供第二衍射光学器件用于补偿分束光束的色差，并且提供收集光学器件用于将分束束束聚焦到图像平面中。
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