公开(公告)号：US07025886B2

公开(公告)日：2006-04-11

申请号：US10473415

申请日：2002-03-25

Applicant: Camilla Viklund ,  Knut Irgum

Inventor： Camilla Viklund ,  Knut Irgum

IPC: B01D15/08

CPC classification number: B01J20/285 ,  B01D15/34 ,  B01J20/26 ,  B01J20/261 ,  B01J20/267 ,  B01J20/28042 ,  B01J2220/82 ,  C08F212/08 ,  C08F257/02

Abstract: The present invention provides porous monoliths with high flow permeability that can be produced by polymerising and divinylbenzene in the presence of an initiator, a carboxy-functionalized nitroxide stable free radical and polymeric porogens. The monoliths produced with these stable free radicals functionalized with carboxylic groups feature porosities very different from those produced by polymerizations involving other SFRs, characterized by very large surface areas in combination with relatively large through-pores. The invention also provides a method for producing the monoliths, a column containing the monoliths and an assay method using the column.

Abstract translation: 本发明提供了在引发剂，羧基官能化的氮氧自由基和聚合物致孔剂的存在下可以通过聚合和二乙烯基苯来制备具有高流动性的多孔整料。 用这些用羧基官能化的这些稳定的自由基产生的整料特征在于与通过涉及其它SFR的聚合产生的那些非常不同的孔隙度，其特征在于具有相当大的通孔的非常大的表面积。 本发明还提供了一种生产整料的方法，一种含有整料的柱和使用该柱的测定方法。

公开(公告)号：US06884345B1

公开(公告)日：2005-04-26

申请号：US09831162

申请日：1999-11-09

Applicant: Knut Irgum ,  Camilla Viklund

Inventor： Knut Irgum ,  Camilla Viklund

IPC: B01J20/285 ,  B01D15/08 ,  B01D15/36 ,  B01J20/26 ,  B01J20/32 ,  B01J39/26 ,  B01J43/00 ,  G01N30/26 ,  G01N30/52 ,  G01N30/88

CPC classification number: B01J39/26 ,  B01D15/364 ,  B01J20/28078 ,  B01J20/286 ,  B01J20/289 ,  B01J20/3092 ,  B01J20/321 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3251 ,  B01J20/3255 ,  B01J20/3278 ,  B01J20/3285 ,  B01J20/3293 ,  B01J41/20 ,  B01J43/00 ,  B01J2220/54 ,  B01J2220/58 ,  B01J2220/82 ,  G01N2030/528

Abstract: A novel sorbent suitable for use as a stationary phase in a chromatography column, the core of which consists of an organic polymer of synthetic or natural origin. Further, the carrier exhibits a plurality of covalently bonded non-aromatic zwitterionic groups on its surface. Additionally, the invention also relates to a method for purifying a particular biological macromolecule, such as a protein or a nucleic acid, by zwitterionic ion exchange chromatography as well as an ion exchange column suitable for use in the zwitterionic ion exchange chromatography.

Abstract translation: 适用于色谱柱中固定相的新型吸附剂，其核心由合成或天然来源的有机聚合物组成。 此外，载体在其表面上显示出多个共价键合的非芳族两性离子基团。 此外，本发明还涉及通过两性离子交换层析纯化特定生物大分子如蛋白质或核酸的方法以及适用于两性离子交换层析的离子交换柱。