公开(公告)号：US20170037350A1

公开(公告)日：2017-02-09

申请号：US15304313

申请日：2015-04-09

Applicant: CORNING INCORPORATED

Inventor： David Alan Kenney ,  Nikolaos Pantelis Kladias ,  Shang-Pin Kwei ,  Aravind Raghavan Rammohan ,  Joseph Christopher Wall ,  Kathy Marie Youngbear

IPC: C12M1/24 ,  C12M1/00

CPC classification number: C12M23/08 ,  B01F9/0021 ,  B01F15/00876 ,  B01F2009/0092 ,  C12M27/20

Abstract: A 5-liter bioreactor vessel enables a substantial increase in the volume of biological media that can be cultivated in the vessel compared to conventional designs. Moreover, when agitated at 1.5× the shaking frequency of a conventional 3-liter bioreactor (i.e., 90 rpm versus 60 rpm), the 5-liter vessel achieves a 19% increase in cell aeration without exceeding the maximum shear stress limit for cell viability. The 5-liter vessel optionally includes a plurality of internal baffles configured to disrupt the liquid vortex and reduce the maximum shear stress transferred to biomedia contained within the vessel.

Abstract translation: 与常规设计相比，5升生物反应器容器能够显着增加在容器中培养的生物培养基的体积。 此外，当以常规3升生物反应器（即90rpm至60rpm）的振荡频率为1.5倍搅拌时，5升容器在不超过细胞活力的最大剪切应力极限的情况下实现细胞曝气增加19％ 。 5升容器可选地包括多个内部挡板，其构造成破坏液体涡流并减少转移到容器内所含生物媒体的最大剪切应力。

公开(公告)号：US20160144360A1

公开(公告)日：2016-05-26

申请号：US14950481

申请日：2015-11-24

Applicant: Corning Incorporated

Inventor： William Joseph Lacey ,  Paul Michael Szlosek ,  Allison Jean Tanner ,  Joseph Christopher Wall ,  Kathy Marie Youngbear

IPC: B01L3/00 ,  B29C51/00 ,  B29C51/10 ,  B29C45/00 ,  B29C51/42

CPC classification number: B01L3/50851 ,  B01L2200/12 ,  B01L2300/0829 ,  B01L2300/0858 ,  B29C45/0001 ,  B29C51/002 ,  B29C51/10 ,  B29C51/428 ,  B29K2023/12 ,  B29K2105/256 ,  B29K2995/0026 ,  B29L2031/752

Abstract: A method of forming microplate includes forming a preform microplate including a deck and a plurality of wells, and reforming at least one well of the plurality of wells to provide a microplate. Each well of the plurality of wells in the preform microplate has a first depth and a first wall thickness. At least one well of the microplate has a second depth and a second wall thickness, where the second depth is greater than the first depth and/or the second wall thickness is less than the first wall thickness.

Abstract translation: 形成微孔板的方法包括形成包括甲板和多个孔的预成型微孔板，以及重整多个孔中的至少一个孔以提供微孔板。 预成型微孔板中的多个孔的每个孔具有第一深度和第一壁厚度。 微孔板的至少一个孔具有第二深度和第二壁厚，其中第二深度大于第一深度和/或第二壁厚度小于第一壁厚度。