公开(公告)号：US20170037350A1

公开(公告)日：2017-02-09

申请号：US15304313

申请日：2015-04-09

Applicant: CORNING INCORPORATED

Inventor： David Alan Kenney ,  Nikolaos Pantelis Kladias ,  Shang-Pin Kwei ,  Aravind Raghavan Rammohan ,  Joseph Christopher Wall ,  Kathy Marie Youngbear

IPC: C12M1/24 ,  C12M1/00

CPC classification number: C12M23/08 ,  B01F9/0021 ,  B01F15/00876 ,  B01F2009/0092 ,  C12M27/20

Abstract: A 5-liter bioreactor vessel enables a substantial increase in the volume of biological media that can be cultivated in the vessel compared to conventional designs. Moreover, when agitated at 1.5× the shaking frequency of a conventional 3-liter bioreactor (i.e., 90 rpm versus 60 rpm), the 5-liter vessel achieves a 19% increase in cell aeration without exceeding the maximum shear stress limit for cell viability. The 5-liter vessel optionally includes a plurality of internal baffles configured to disrupt the liquid vortex and reduce the maximum shear stress transferred to biomedia contained within the vessel.

Abstract translation: 与常规设计相比，5升生物反应器容器能够显着增加在容器中培养的生物培养基的体积。 此外，当以常规3升生物反应器（即90rpm至60rpm）的振荡频率为1.5倍搅拌时，5升容器在不超过细胞活力的最大剪切应力极限的情况下实现细胞曝气增加19％ 。 5升容器可选地包括多个内部挡板，其构造成破坏液体涡流并减少转移到容器内所含生物媒体的最大剪切应力。