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1.发明公开公开(公告)号：US20230313232A1
公开(公告)日：2023-10-05
申请号：US18175790
申请日：2023-02-28
Applicant: CORNING INCORPORATED
Inventor： Carine Cerato-Noyerie , Samantha Sophie Catherine Haller , Davis Henry , Marylène Denise Madeleine Pécheul , Corinne Walerack
Abstract: Methods and systems are provided for transfecting cells using real-time, continuous transfection of cells. In some aspects, the methods can be applied for the continuous production of non-viral vector nucleic acid complexes. The systems and methods include a passive mixing fluidic module with at least two inlets, a plurality of mixing elements, and an outlet to provide a continuous flow of transfection complexes to a cell reactor. The transfection agent and nucleic acid are passively mixed and then provided to cells in a continuous flow of cell medium. In some aspects, the flow of cell medium perfusing through the cell reactor recirculates. The system and the methods of the present disclosure provide for highly reproducible and scalable transfection with a low coefficient of variation.
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公开(公告)号：US20220186178A1
公开(公告)日：2022-06-16
申请号：US17558844
申请日：2021-12-22
Applicant: CORNING INCORPORATED
Inventor： David Henry , Martial Hervy , Corinne Walerack
IPC: C12N5/00
Abstract: A cell culture article, including: a substrate comprising a polygalacturonic acid compound selected from at least one of: pectic acid; partially esterified pectic acid having a degree of esterification from 1 to 40 mol %, or salts thereof; and an adhesion polymer on the surface of the polygalacturonic acid compound. A method of making and using the article are also disclosed.
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公开(公告)号：US20180179489A1
公开(公告)日：2018-06-28
申请号：US15579739
申请日：2016-06-08
Applicant: Corning Incorporated
Inventor： Stephen Joseph Caracci , David Henry , Corinne Walerack , Yue Zhou
IPC: C12N5/00 , C12N5/0775 , C12N5/071
CPC classification number: C12N5/0075 , C08L5/00 , C12N5/0663 , C12N5/0686 , C12N5/0688 , C12N2531/00 , C12N2533/18 , C12N2533/70
Abstract: A cell culture article is provided. The cell culture substrate includes a polygalacturonic acid compound selected from at least one of: pectic acid or salts thereof, and partially esterified pectic acid having a degree of esterification from 1 to 40 mol % or salts thereof. The polygalacturonic acid compound is crosslinked with a divalent cation and the divalent cation concentration ranges from 0.5 to 2 g/1 of the substrate.
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公开(公告)号：US20210062142A1
公开(公告)日：2021-03-04
申请号：US17022764
申请日：2020-09-16
Applicant: CORNING INCORPORATED
Inventor： Ye Fang , Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , David Henry , Martial Hervy , Corinne Walerack , Yue Zhou
IPC: C12N5/00 , C08J3/24 , C09D167/00
Abstract: A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.
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公开(公告)号：US10203320B2
公开(公告)日：2019-02-12
申请号：US14437275
申请日：2013-11-15
Applicant: Corning Incorporated
Inventor： Oberon Denaci Deichmann , Ye Fang , Ann MeeJin Ferrie , David Henry , Haibei Hu , Corinne Walerack , Qi Wu
Abstract: A label-free resonant waveguide grating biosensor imager system for measuring beat patterns and dynamic mass redistribution (DMR) signals of cultured cardiomyocytes in the absence and presence of a drug molecule. The disclosure also provides a method using the imager system for analyzing the beat patterns and the DMR signals of the cardiomyocytes to assess drug-induced cardiotoxicity.
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Patent Agency Ranking
公开(公告)号：US20240271085A1
公开(公告)日：2024-08-15
申请号：US18028961
申请日：2021-09-14
Applicant: CORNING INCORPORATED
Inventor： David Henry , Corinne Walerack
IPC: C12N5/00
CPC classification number: C12N5/0068 , C12N2533/40 , C12N2537/10 , C12N2539/10
Abstract: Activated dissolvable supports are provided comprising an ionotropically crosslinked compound comprising a polymer material having at least one repeating unit comprising an ionically crosslinked carboxylic acid group, and an activated hydroxyl group, wherein the hydroxyl group is activated by N,N′-disuccinimidyl carbonate (DSC) or N-hydroxy succinimidyl chloroformate in a solvent to form succinimidyl carbonate groups for ligand binding. Methods of forming activated dissolvable supports, culturing cells on activated dissolvable supports, and harvesting cells from dissolvable supports are provided.
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公开(公告)号：US20200291348A1
公开(公告)日：2020-09-17
申请号：US16765722
申请日：2018-11-20
Applicant: CORNING INCORPORATED
Inventor： Marie-Maud Bear , David Henry , Martial Hervy , Marylène Denise Madeleine Pécheul , Corinne Walerack
IPC: C12N5/00
Abstract: A dissolvable foam scaffold for cell culture is provided herein. The dissolvable foam scaffold includes an ionotropically crosslinked polygalacturonic acid compound selected from at least one of: pectic acid; partially esterified pectic acid, partially amidated pectic acid and salts thereof, and at least one first water-soluble polymer having surface activity.
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公开(公告)号：US20160145567A1
公开(公告)日：2016-05-26
申请号：US14899394
申请日：2014-06-23
Applicant: CORNING INCORPORATED
Inventor： David Henry , Martial Hervy , Corinne Walerack
IPC: C12N5/00
CPC classification number: C12N5/0075 , C12N2509/00 , C12N2531/00 , C12N2533/30 , C12N2533/70
Abstract: A cell culture article, including: a substrate comprising a polygalacturonic acid compound selected from at least one of: pectic acid; partially esterified pectic acid having a degree of esterification from 1 to 40 mol %, or salts thereof; and an adhesion polymer on the surface of the polygalacturonic acid compound. A method of making and using the article are also disclosed.
Abstract translation: 一种细胞培养物品，包括：包含选自以下中的至少一种的聚半乳糖醛酸化合物的底物：果胶酸; 酯化度为1〜40摩尔％的部分酯化果胶酸，或其盐; 和聚半乳糖醛酸化合物表面上的粘合聚合物。 还公开了制造和使用制品的方法。
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公开(公告)号：US20240026287A1
公开(公告)日：2024-01-25
申请号：US18226559
申请日：2023-07-26
Applicant: CORNING INCORPORATED
Inventor： Ye Fang , Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , David Henry , Martial Hervy , Corinne Walerack , Yue Zhou
IPC: C12N5/00 , C08J3/24 , C09D167/00
CPC classification number: C12N5/0075 , C12N5/0068 , C08J3/24 , C09D167/00 , C12N2533/40 , C12N2533/50 , C12N2537/10 , C12N2533/32 , C12N2533/74
Abstract: A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.
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公开(公告)号：US10941312B2
公开(公告)日：2021-03-09
申请号：US16196516
申请日：2018-11-20
Applicant: Corning Incorporated
Inventor： Anthony Glenn Frutos , David Henry , Corinne Walerack
IPC: C09D151/00 , C09D189/00 , B05D3/06 , C08F220/20 , C08F220/28
Abstract: A method for coating a surface of a cell culture article includes dissolving a polymer having a covalently attached polypeptide in an aqueous solution to produce a polymer solution. The polymer is formed from monomers selected to form a polymer having a linear backbone, wherein the polymer is crosslink free. The weight percentage of the polypeptide relative to the polymer conjugated to the polypeptide is sufficiently high to render the polymer conjugated to the polypeptide water soluble. The aqueous solution is substantially free of organic solvents. The method further includes (i) disposing the polymer solution on the surface of the cell culture article to produce a coated article; and (ii) subjecting the coated article to sufficient heat or electromagnetic radiation to attach the polymer conjugated to a polypeptide to the surface of the cell culture article.
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