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13 条记录 (耗时 0.032 秒)

    Method for Amplification of Target Nucleic Acids Using a Multi-Primer Approach
    1.
    发明申请
    Method for Amplification of Target Nucleic Acids Using a Multi-Primer Approach 审中-公开
    使用多引物方法扩增靶核酸的方法

    公开(公告)号：US20130045894A1

    公开(公告)日：2013-02-21

    申请号：US13586042

    申请日：2012-08-15

    申请人： Bruno Frey ,  Irene Labaere

    发明人： Bruno Frey ,  Irene Labaere

    IPC分类号： C40B50/06 ,  C40B40/06

    CPC分类号： C12Q1/686 ,  C12Q2537/143 ,  C12Q2549/119

    摘要： The present invention is a method for amplification of target nucleic acids wherein a first and second primer set is used to amplify a target nucleic acid in a single amplification reaction. The primers of the first primer set generate a first amplification product that serves as a template for amplification by the primers of the second primer set due to the incorporation of a first and second tag sequence added to the target nucleic acid from the forward and reverse primers of the first primer set to which the primers of the second primer set can hybridize to its complement. Additional sequences are thereby added to the resulting target nucleic acid amplicons because of further amplification from the first amplification products by the primers of the second primer set.

    摘要翻译： 本发明是用于扩增靶核酸的方法，其中在单次扩增反应中使用第一和第二引物组来扩增靶核酸。 第一引物组的引物产生第一扩增产物，其作为用于扩增第二引物组的引物的模板，这是由于引入加入靶核酸的第一和第二标签序列来自正向和反向引物 的第一引物组，其中第二引物组的引物可以与其补体杂交。 因为由于第二引物组的引物来自第一扩增产物的进一步扩增，因此将附加的序列加入到所得的靶核酸扩增子中。

    CpG island sequencing
    4.
    发明申请
    CpG island sequencing 审中-公开
    CpG岛测序

    公开(公告)号：US20100075331A1

    公开(公告)日：2010-03-25

    申请号：US12584435

    申请日：2009-09-03

    申请人： Gisela Betzl ,  Bruno Frey ,  Epameinondas Fritzilas ,  Bernhard Horsthemke ,  Sven Rahmann ,  Michael Zeschnigk

    发明人： Gisela Betzl ,  Bruno Frey ,  Epameinondas Fritzilas ,  Bernhard Horsthemke ,  Sven Rahmann ,  Michael Zeschnigk

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q1/6869 ,  C12Q2523/125 ,  C12Q2521/307

    摘要： The present invention is directed to a method for analyzing the methylation status of a genomic DNA sample, comprising the steps of (i) fragmenting said sample and enriching said sample for sequences comprising CpG islands, (ii) generating a single stranded DNA library, (iii) subjecting said sample to Bisulfite treatment, (iv) clonally amplifying individual members of said single stranded DNA library by means of emulsion PCR, and (v) sequencing said amplified clonally amplified single stranded DNA library.

    摘要翻译： 本发明涉及一种用于分析基因组DNA样品的甲基化状态的方法，包括以下步骤：（i）将所述样品分段并富集所述样品以获得包含CpG岛的序列，（ii）产生单链DNA文库（ iii）使所述样品经受亚硫酸氢盐处理，（iv）通过乳液PCR克隆扩增所述单链DNA文库的单个成员，和（v）对所述扩增的克隆扩增的单链DNA文库进行测序。

    Type II restriction endonuclease SexAI
    5.
    发明授权
    Type II restriction endonuclease SexAI 失效
    II型限制性内切酶SexAI

    公开(公告)号：US5354669A

    公开(公告)日：1994-10-11

    申请号：US94374

    申请日：1993-07-19

    申请人： Klaus Kaluza ,  Johannes Auer ,  Bruno Frey

    发明人： Klaus Kaluza ,  Johannes Auer ,  Bruno Frey

    IPC分类号： C12N9/22 ,  C12P19/34

    CPC分类号： C12N9/22

    摘要： The new type II restriction endonuclease SexAI has the following recognition sequence and preferably cleaves at the cleavage site defined by the mark: ##STR1## It is preferably obtainable from microorganisms of the genus Streptomyces.

    摘要翻译： 新型II型限制性内切核酸酶SexAI具有以下识别序列，优选在由标记：链霉菌属的无机物定义的切割位点切割。

    Polymerase chimeras
    6.
    发明授权
    Polymerase chimeras 有权
    聚合酶嵌合体

    公开(公告)号：US07244602B2

    公开(公告)日：2007-07-17

    申请号：US10456129

    申请日：2003-06-06

    申请人： Bruno Frey ,  Britta Villbrandt ,  Dietmar Schomburg ,  Harald Sobek ,  Waltraud Ankenbauer

    发明人： Bruno Frey ,  Britta Villbrandt ,  Dietmar Schomburg ,  Harald Sobek ,  Waltraud Ankenbauer

    IPC分类号： C12N9/12 ,  C12Q1/68

    CPC分类号： C12N9/1252 ,  C07K2319/00

    摘要： The invention concerns polymerase chimeras which are composed of amino acid fragments representing domains and which combine properties of naturally occurring polymerases that are advantageous with regard to a particular application. It has surprisingly turned out that the domains from the various enzymes are active in the chimeras and exhibit cooperative behavior. In addition the present invention concerns a process for the production of the chimeras according to the invention and the use of these chimeras for the synthesis of nucleic acids e.g. during a polymerase chain reaction. Moreover the present invention concerns a kit which contains the polymerase chimeras according to the invention.

    摘要翻译： 本发明涉及聚合酶嵌合体，其由表示结构域的氨基酸片段组成，并且其结合对于特定应用有利的天然存在的聚合酶的性质。 令人惊讶的是，各种酶的结构域在嵌合体中是活性的，并表现出合作行为。 此外，本发明涉及根据本发明的嵌合体的生产方法和这些嵌合体用于合成核酸的用途，例如， 在聚合酶链反应期间。 此外，本发明涉及包含根据本发明的聚合酶嵌合体的试剂盒。

    Thermolabile uracil-DNA-glycosylas, process for its preparation and use for removing uracil from DNA
    9.
    发明授权
    Thermolabile uracil-DNA-glycosylas, process for its preparation and use for removing uracil from DNA 有权
    Thermolabile尿嘧啶-DNA-糖基，其制备方法和用于从DNA中去除尿嘧啶

    公开(公告)号：US06187575B1

    公开(公告)日：2001-02-13

    申请号：US09077312

    申请日：1998-08-06

    申请人： Harald Sobek ,  Manfred Schmidt ,  Bruno Frey ,  Klaus Kaluza

    发明人： Harald Sobek ,  Manfred Schmidt ,  Bruno Frey ,  Klaus Kaluza

    IPC分类号： C12N914

    CPC分类号： C12Q1/6848 ,  C12N9/2497 ,  C12Q2521/531

    摘要： Thermolabile enzyme with uracil-DNA-glycosylase activity which is in particular characterized by a high degree of purity, short half-lives and a content of contaminating foreign activities of less than 2%, a process for its isolation as well as the use thereof to remove the base uracil from DNA and in particular from PCR products containing uracil. The enzyme is obtainable from gram-positive microorganisms such as e.g. Arthrobacter or Micrococcus.

    摘要翻译： 具有尿嘧啶-DNA-糖基化酶活性的耐热不溶性酶，其特征在于其纯度高，半衰期短，外来污染物含量小于2％，其分离方法及其用途 从DNA中去除碱基尿嘧啶，特别是从含有尿嘧啶的PCR产物中除去。 该酶可从革兰氏阳性微生物获得，例如， 节杆菌属或微球菌属。