公开(公告)号：US06865939B2

公开(公告)日：2005-03-15

申请号：US10245224

申请日：2002-09-16

Applicant: Brian J. Kirby ,  Timothy Jon Shepodd

Inventor： Brian J. Kirby ,  Timothy Jon Shepodd

IPC: B05D3/02 ,  B05D5/08 ,  G01F3/08

CPC classification number: B05D5/083 ,  B05D3/0254 ,  B05D2203/30 ,  B05D2254/04

Abstract: A method for surface modification of microchannels and capillaries. The method produces a chemically inert surface having a lowered surface free energy and improved frictional properties by attaching a fluorinated alkane group to the surface. The coating is produced by hydrolysis of a silane agent that is functionalized with either alkoxy or chloro ligands and an uncharged C3-C10 fluorinated alkane chain. It has been found that the extent of surface coverage can be controlled by controlling the contact time from a minimum of about 2 minutes to a maximum of 120 minutes for complete surface coverage.

Abstract translation: 微通道和毛细管表面改性的方法。 该方法通过将氟化烷烃基团附着在表面上产生具有降低的表面自由能和改善摩擦性能的化学惰性表面。 涂层通过用烷氧基或氯配体官能化的硅烷试剂的水解和不带电的C 3 -C 10氟化烷烃链来水解制备。 已经发现，通过将接触时间从最小约2分钟控制到最大120分钟来完成表面覆盖，可以控制表面覆盖的程度。

公开(公告)号：US06952962B2

公开(公告)日：2005-10-11

申请号：US10141906

申请日：2002-05-08

Applicant: Ernest F. Hasselbrink, Jr. ,  Jason E. Rehm ,  Timothy J. Shepodd ,  Brian J. Kirby

Inventor： Ernest F. Hasselbrink, Jr. ,  Jason E. Rehm ,  Timothy J. Shepodd ,  Brian J. Kirby

IPC: F15C5/00 ,  F16K99/00 ,  G01F3/06 ,  G01F3/24 ,  G01F3/08

CPC classification number: F16K99/0001 ,  F15C5/00 ,  F16K99/0005 ,  F16K99/0011 ,  F16K99/0017 ,  F16K99/003 ,  F16K99/0034 ,  F16K99/004 ,  F16K99/0044 ,  F16K99/0051 ,  F16K99/0057 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0084 ,  G01F3/06 ,  G01F3/24 ,  Y10T428/2933

Abstract: A cast-in-place and lithographically shaped mobile, monolithic polymer element for fluid flow control in microfluidic devices and method of manufacture. Microfluid flow control devices, or microvalves that provide for control of fluid or ionic current flow can be made incorporating a cast-in-place, mobile monolithic polymer element, disposed within a microchannel, and driven by fluid pressure (either liquid or gas) against a retaining or sealing surface. The polymer elements are made by the application of lithographic methods to monomer mixtures formulated in such a way that the polymer will not bond to microchannel walls. The polymer elements can seal against pressures greater than 5000 psi, and have a response time on the order of milliseconds. By the use of energetic radiation it is possible to depolymerize selected regions of the polymer element to form shapes that cannot be produced by conventional lithographic patterning and would be impossible to machine.

Abstract translation: 用于微流体装置中流体流动控制的现浇和光刻形移动式整体式聚合物元件及其制造方法。 提供控制流体或离子电流的微流量控制装置或微型阀可以结合设置在微通道内的现浇可移动整体式聚合物元件并由流体压力（液体或气体）驱动 保持或密封表面。 通过将光刻方法应用于以聚合物不会结合到微通道壁的方式配制的单体混合物来制备聚合物元件。 聚合物元件可以密封大于5000psi的压力，并且具有大约毫秒级的响应时间。 通过使用高能辐射，可以解聚聚合物元件的选定区域以形成不能通过常规平版印刷图案产生的形状，并且不可能加工。

公开(公告)号：US07264723B2

公开(公告)日：2007-09-04

申请号：US10443491

申请日：2003-05-22

Applicant: Anup K. Singh ,  Brian J. Kirby ,  Timothy J. Shepodd

Inventor： Anup K. Singh ,  Brian J. Kirby ,  Timothy J. Shepodd

IPC: B01D69/10

CPC classification number: B01D67/0006 ,  B01D61/28 ,  B01D71/40 ,  B01D2323/30 ,  B01D2323/345 ,  B01D2325/02 ,  B01D2325/028 ,  B01D2325/08 ,  B01F13/0059 ,  B01F13/0093 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/0681 ,  G01N2001/4016 ,  Y10T156/10 ,  Y10T436/118339 ,  Y10T436/255

Abstract: Laser-induced phase-separation polymerization of a porous acrylate polymer is used for in-situ fabrication of dialysis membranes inside glass microchannels. A shaped 355 nm laser beam is used to produce a porous polymer membrane with a thickness of about 15 μm, which bonds to the glass microchannel and form a semi-permeable membrane. Differential permeation through a membrane formed with pentaerythritol triacrylate was observed and quantified by comparing the response of the membrane to fluorescein and fluorescently tagging 200 nm latex microspheres. Differential permeation was observed and quantified by comparing the response to rhodamine 560 and lactalbumin protein in a membrane formed with SPE-methylene bisacrylamide. The porous membranes illustrate the capability for the present technique to integrate sample cleanup into chip-based analysis systems.

Abstract translation: 多孔丙烯酸酯聚合物的激光诱导相分离聚合用于玻璃微通道内透析膜的原位制备。 使用形状为355nm的激光束产生厚度约15μm的多孔聚合物膜，其结合到玻璃微通道并形成半透膜。 通过比较膜与荧光素的反应和荧光标记200nm胶乳微球，观察到通过由季戊四醇三丙烯酸酯形成的膜的差异渗透和定量。 通过比较用SPE-亚甲基双丙烯酰胺形成的膜中的罗丹明560和乳白蛋白的响应，观察并定量差异渗透。 多孔膜说明了本技术将样品清理整合到基于芯片的分析系统中的能力。

公开(公告)号：US07022381B2

公开(公告)日：2006-04-04

申请号：US10610093

申请日：2003-06-30

Applicant: Brian J. Kirby ,  David S. Reichmuth ,  Timothy J. Shepodd

Inventor： Brian J. Kirby ,  David S. Reichmuth ,  Timothy J. Shepodd

IPC: C08F2/46 ,  C08J7/18 ,  B05D7/22

CPC classification number: F16K99/0001 ,  F15C5/00 ,  F16K99/0025 ,  F16K99/0049 ,  F16K2099/0074

Abstract: A microvalve having a cast-in-place and lithographically shaped mobile, polymer monolith for fluid flow control in microfluidic devices and method of manufacture. The microvalve contains a porous fluorinated polymer monolithic element whose pores are filled with an electrically insulating, high dielectric strength fluid, typically a perfluorinated liquid. This combination provides a microvalve that combines high dielectric strength with extremely low electrical conductivity. These microvalves have been shown to have resistivities of at least 100 GΩ and are compatible with solvents such as water at a pH between 2.7 and 9.0, 1-1 propanol, acetonitrile, and acetone.

Abstract translation: 具有现浇和光刻形移动式聚合物整料的微型阀，用于微流体装置中的流体流动控制和制造方法。 微型阀包含多孔氟化聚合物整体元件，其孔被电绝缘的高介电强度流体（通常为全氟化液体）填充。 这种组合提供了一种结合了高介电强度和极低导电性的微型阀。 已经显示这些微型阀具有至少100Gega的电阻率，并且与溶剂（例如pH在2.7至9.0之间的水，1-1丙醇，乙腈和丙酮）相容。

公开(公告)号：US07754077B1

公开(公告)日：2010-07-13

申请号：US11825590

申请日：2007-07-05

Applicant: Anup K. Singh ,  Brian J. Kirby ,  Timothy J. Shepodd

Inventor： Anup K. Singh ,  Brian J. Kirby ,  Timothy J. Shepodd

IPC: B01D69/10

CPC classification number: B01D67/0006 ,  B01D61/28 ,  B01D71/40 ,  B01D2323/30 ,  B01D2323/345 ,  B01D2325/02 ,  B01D2325/028 ,  B01D2325/08 ,  B01F13/0059 ,  B01F13/0093 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/0681 ,  G01N2001/4016 ,  Y10T156/10 ,  Y10T436/118339 ,  Y10T436/255

Abstract: Laser-induced phase-separation polymerization of a porous acrylate polymer is used for in-situ fabrication of dialysis membranes inside glass microchannels. A shaped 355 nm laser beam is used to produce a porous polymer membrane with a thickness of about 15 μm, which bonds to the glass microchannel and forms a semi-permeable membrane. Differential permeation through a membrane formed with pentaerythritol triacrylate was observed and quantified by comparing the response of the membrane to fluorescein and fluorescently tagging 200 nm latex microspheres. Differential permeation was observed and quantified by comparing the response to rhodamine 560 and lactalbumin protein in a membrane formed with SPE-methylene bisacrylamide. The porous membranes illustrate the capability for the present technique to integrate sample cleanup into chip-based analysis systems.

Abstract translation: 多孔丙烯酸酯聚合物的激光诱导相分离聚合用于玻璃微通道内透析膜的原位制备。 使用成形的355nm激光束产生厚度约15μm的多孔聚合物膜，其结合到玻璃微通道并形成半透膜。 通过比较膜与荧光素的反应和荧光标记200nm胶乳微球，观察到通过由季戊四醇三丙烯酸酯形成的膜的差异渗透和定量。 通过比较用SPE-亚甲基双丙烯酰胺形成的膜中的罗丹明560和乳白蛋白的响应，观察并定量差异渗透。 多孔膜说明了本技术将样品清理整合到基于芯片的分析系统中的能力。

公开(公告)号：US07534315B1

公开(公告)日：2009-05-19

申请号：US11825600

申请日：2007-07-05

Applicant: Anup K. Singh ,  Brian J. Kirby ,  Timothy J. Shepodd

Inventor： Anup K. Singh ,  Brian J. Kirby ,  Timothy J. Shepodd

IPC: B32B37/00

CPC classification number: B01D67/0006 ,  B01D61/28 ,  B01D71/40 ,  B01D2323/30 ,  B01D2323/345 ,  B01D2325/02 ,  B01D2325/028 ,  B01D2325/08 ,  B01F13/0059 ,  B01F13/0093 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/0681 ,  G01N2001/4016 ,  Y10T156/10 ,  Y10T436/118339 ,  Y10T436/255

Abstract: Laser-induced phase-separation polymerization of a porous acrylate polymer is used for in-situ fabrication of dialysis membranes inside glass microchannels. A shaped 355 nm laser beam is used to produce a porous polymer membrane with a thickness of about 15 μm, which bonds to the glass microchannel and forms a semi-permeable membrane. Differential permeation through a membrane formed with pentaerythritol triacrylate was observed and quantified by comparing the response of the membrane to fluorescein and fluorescently tagging 200 nm latex microspheres. Differential permeation was observed and quantified by comparing the response to rhodamine 560 and lactalbumin protein in a membrane formed with SPE-methylene bisacrylamide. The porous membranes illustrate the capability for the present technique to integrate sample cleanup into chip-based analysis systems.

Abstract translation: 多孔丙烯酸酯聚合物的激光诱导相分离聚合用于玻璃微通道内透析膜的原位制备。 使用成形的355nm激光束来制造厚度约15μm的多孔聚合物膜，其结合到玻璃微通道并形成半透膜。 通过比较膜与荧光素的反应和荧光标记200nm胶乳微球，观察到通过由季戊四醇三丙烯酸酯形成的膜的差异渗透和定量。 通过比较用SPE-亚甲基双丙烯酰胺形成的膜中的罗丹明560和乳白蛋白的响应，观察并定量差异渗透。 多孔膜说明了本技术将样品清理整合到基于芯片的分析系统中的能力。

公开(公告)号：US06988402B2

公开(公告)日：2006-01-24

申请号：US10655337

申请日：2003-09-04

Applicant: Ernest F. Hasselbrink, Jr. ,  Jason E. Rehm ,  Timothy J. Shepodd ,  Brian J. Kirby

Inventor： Ernest F. Hasselbrink, Jr. ,  Jason E. Rehm ,  Timothy J. Shepodd ,  Brian J. Kirby

IPC: G01F3/08

CPC classification number: F16K99/0001 ,  F15C5/00 ,  F16K99/0005 ,  F16K99/0011 ,  F16K99/0017 ,  F16K99/003 ,  F16K99/0034 ,  F16K99/004 ,  F16K99/0044 ,  F16K99/0051 ,  F16K99/0057 ,  F16K99/0059 ,  F16K2099/0074 ,  F16K2099/0084 ,  G01F3/06 ,  G01F3/24 ,  Y10T428/2933

Abstract: A cast-in-place and lithographically shaped mobile, monolithic polymer element for fluid flow control in microfluidic devices and method of manufacture. Microfluid flow control devices, or microvalves that provide for control of fluid or ionic current flow can be made incorporating a cast-in-place, mobile monolithic polymer element, disposed within a microchannel, and driven by fluid pressure (either liquid or gas) against a retaining or sealing surface. The polymer elements are made by the application of lithographic methods to monomer mixtures formulated in such a way that the polymer will not bond to microchannel walls. The polymer elements can seal against pressures greater than 5000 psi, and have a response time on the order of milliseconds. By the use of energetic radiation it is possible to depolymerize selected regions of the polymer element to form shapes that cannot be produced by conventional lithographic patterning and would be impossible to machine.