公开(公告)号：US20170355981A1

公开(公告)日：2017-12-14

申请号：US15529881

申请日：2014-11-26

申请人： BGI SHENZHEN ,  BGI SHENZHEN CO., LIMITED

发明人： Yuan JIANG ,  Qiaoling LI ,  Andrei ALEXEEV ,  Evan HUROWITZ ,  Xia ZHAO ,  Tong WANG ,  Chao DONG ,  Dong LI ,  Radoje DRMANAC ,  Wenwei ZHANG ,  Hui JIANG

IPC分类号： C12N15/10 ,  B01J19/00

摘要： A method and reagent for constructing a nucleic acid double-linker single-strand cyclic library. The method comprises: breaking a nucleic acid into nucleic acid fragments; connecting a first linker sequence; producing by amplification a first product provided with the first linker sequence at either end, where a U nucleobase site is provided on primer sequences and a nicking enzyme recognition sequence is either provided or not provided on same, and a first affinity tag is provided on one of the primer sequences; using USER enzyme to cleave the first product; cyclizing the cleaved first product; treating the cyclization product with either a phosphatase or a nicking enzyme; using a solid-phase vector for combination with a cyclized molecule; performing a restrictive gap translation reaction; removing by digestion any portion that did not undergo the restrictive gap translation reaction; connecting a second linker sequence; producing by amplification a second product provided with the second linker sequence at either end; denaturing the second product, and cyclizing a single-strand nucleic acid molecule. The method allows an increase in the length of library insert fragments, a simplified library construction process, reduced library construction time, and reduced library construction costs.

公开(公告)号：US20180180567A1

公开(公告)日：2018-06-28

申请号：US15738114

申请日：2016-06-23

申请人： BGI SHENZHEN ,  BGI SHENZHEN CO., LIMITED

发明人： Handong LI ,  Jianxun LIN ,  Quanxin YUN ,  Shaohua XIANG ,  Radoje DRMANAC ,  Snezana DRMANAC ,  Yongwei ZHANG

IPC分类号： G01N27/327 ,  C12Q1/6869

CPC分类号： G01N27/3276 ,  B81B7/02 ,  C12Q1/6869 ,  G01N27/3277 ,  G01N27/3278 ,  G01N33/48721 ,  C12Q2521/543 ,  C12Q2563/116 ,  C12Q2565/607 ,  C12Q2565/631 ,  C12Q2521/101 ,  C12Q2535/122 ,  C12Q2537/157

摘要： Provided is a microwell electrode, comprising one or more first electrodes (301); one or more second electrodes (303) each arranged opposite to one first electrode (301), wherein a channel (601) is provided between each first electrode and the second electrode opposite thereto, and the channel (601) has at least one end in communication with a chamber; and one or more guiding electrodes (501) located in the chamber (401). The microwell electrode electrode can sensitively detect a signal and improve the read length of a sequencer greatly. The invention further relates to a method for manufacturing the micro-porous electrode, a microwell electrode array, a sensor chip, a sequencing system, and a method for analysis of a chemical substance and a nucleic acid molecule based on the microwell electrode.

公开(公告)号：US20170349893A1

公开(公告)日：2017-12-07

申请号：US15529867

申请日：2014-11-26

申请人： BGI SHENZHEN ,  BGI SHENZHEN CO., LIMITED

发明人： Yuan JIANG ,  Xia ZHAO ,  Andrei ALEXEEV ,  Radoje DRMANAC ,  Wenwei ZHANG ,  Hui JIANG

IPC分类号： C12N15/10

CPC分类号： C12N15/1068 ,  C12N15/10 ,  C12N15/11 ,  C12Q1/68 ,  C40B50/06 ,  C40B50/18

摘要： A method and reagent for constructing a nucleic acid double-joint single-strand cyclical library. The method comprises: breaking a nucleic acid into nucleic acid fragments; connecting a first linker sequence; producing by amplification a first product provided with the first linker sequence at either end, where a U nucleobase is provided on a primer sequence; using USER enzyme to cleave the first product and cyclizing to produce a gap; or, a nicking enzyme recognition sequence is also provided on the primer sequence, using the USER enzyme to cleave the first product, cyclizing and using a nicking enzyme for nicking to produce a nick; performing a restrictive nick/gap translation reaction from the nick or the gap; removing by digestion any portion that did not undergo the restrictive nick/gap translation reaction; connecting a second linker sequence; producing by amplification a second product provided with the second linker sequence at either end; denaturing the second product, and using a mediated sequence for cyclization of a single-strand nucleic acid molecule. The method allows an increase in the length of library insert fragments and obviates the need for gel extraction; the single-strand nucleic acid molecule can be cyclized directly when denatured with heat.

公开(公告)号：US20180044668A1

公开(公告)日：2018-02-15

申请号：US15519149

申请日：2015-10-13

申请人： BGI SHENZHEN CO., LIMITED

发明人： Yuan JIANG ,  Radoje DRMANAC ,  Evan HUROWITZ ,  Andrei ALEXEEV ,  Xia ZHAO ,  Jie RUAN

IPC分类号： C12N15/10 ,  C12Q1/68

CPC分类号： C12N15/1093 ,  C12Q1/6855 ,  C12Q1/6869 ,  C12Q1/6874 ,  C40B40/06 ,  C40B50/06 ,  C12Q2521/501 ,  C12Q2525/191 ,  C12Q2565/50 ,  C12Q2565/631

摘要： The present invention provides a novel method for ligating an adapter to a target polynucleotide and methods of generating a library of mate-pair polynucleotide constructs that employ such a ligation method. Libraries and arrays comprising mate-pair polynucleotide constructs, and methods of sequencing libraries and arrays comprising mate-pair polynucleotide constructs, are also provided.

公开(公告)号：US20170233728A1

公开(公告)日：2017-08-17

申请号：US15519112

申请日：2014-10-14

申请人： BGI Shenzhen Co., Limited

发明人： Yuan JIANG ,  Chunyu GENG ,  Xia ZHAO ,  Shujin FU ,  Lingyu HE ,  Yaqiao LI ,  Xiaoshan SU ,  Fanzi WU ,  Wenwei ZHANG ,  Hui JIANG ,  Andrei ALEXEEV ,  Radoje DRMANAC

IPC分类号： C12N15/10 ,  C12N9/12 ,  C12N9/16

CPC分类号： C12N15/1093 ,  C12N9/1205 ,  C12N9/1252 ,  C12N9/16 ,  C12N15/11 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q2525/191 ,  C12Y207/01078 ,  C12Y207/07007 ,  C12Y301/03001 ,  C40B40/06 ,  C40B50/06 ,  C12Q2521/531 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/307 ,  C12Q2535/122 ,  C12Q2537/162 ,  C12Q2563/131

摘要： Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5′ end of the long strand has a phosphoric acid modification, and the 3′ end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3′ end thereof can be in a complementary pairing with the 5′ end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.

公开(公告)号：US20230175047A1

公开(公告)日：2023-06-08

申请号：US17611099

申请日：2020-05-14

申请人： BGI SHENZHEN ,  MGI TECH CO., LTD.

发明人： Ao CHEN ,  Xun XU ,  Sha LIAO ,  Jin YANG ,  Longqi LIU ,  Ou WANG ,  Yuxiang LI ,  Guoxin TANG ,  Yuan JIANG ,  Chongjun XU ,  Ming NI ,  Wenwei ZHANG ,  Radoje DRMANAC ,  Snezana DRMANAC

IPC分类号： C12Q1/6841 ,  C12Q1/6837 ,  C12N15/10

CPC分类号： C12Q1/6841 ,  C12Q1/6837 ,  C12N15/1093

摘要： Provided are a method for detecting spatial information of nucleic acids in a sample, as well as a nucleic acid array used in the method and a method for producing the nucleic acid array.