公开(公告)号：US20170233728A1

公开(公告)日：2017-08-17

申请号：US15519112

申请日：2014-10-14

申请人： BGI Shenzhen Co., Limited

发明人： Yuan JIANG ,  Chunyu GENG ,  Xia ZHAO ,  Shujin FU ,  Lingyu HE ,  Yaqiao LI ,  Xiaoshan SU ,  Fanzi WU ,  Wenwei ZHANG ,  Hui JIANG ,  Andrei ALEXEEV ,  Radoje DRMANAC

IPC分类号： C12N15/10 ,  C12N9/12 ,  C12N9/16

CPC分类号： C12N15/1093 ,  C12N9/1205 ,  C12N9/1252 ,  C12N9/16 ,  C12N15/11 ,  C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q2525/191 ,  C12Y207/01078 ,  C12Y207/07007 ,  C12Y301/03001 ,  C40B40/06 ,  C40B50/06 ,  C12Q2521/531 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2525/307 ,  C12Q2535/122 ,  C12Q2537/162 ,  C12Q2563/131

摘要： Provided is a linker element and a method of using the linker element to construct a sequencing library, wherein the linker element consists of a linker A and a linker B, the linker A is obtained through the complementary pairing of a long nucleic acid strand and a short nucleic acid strand, the 5′ end of the long strand has a phosphoric acid modification, and the 3′ end of the short strand has an enclosed modification, with enzyme sites in the short strand; and the linker B is a nucleic acid single strand, and the 3′ end thereof can be in a complementary pairing with the 5′ end of the long strand of the linker A. Using the linker element of the present invention for constructing a sequencing library ensures the linking directionality of the linkers while solving the problems of fragment interlinking, linker self-linking and low linking efficiency, and reducing the purification reaction between steps, shortening the linking time and reducing costs.