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    Phosphoserine phosphatase gene of coryneform bacteria
    4.
    发明申请
    Phosphoserine phosphatase gene of coryneform bacteria 有权

    公开(公告)号：US20030114656A1

    公开(公告)日：2003-06-19

    申请号：US10321382

    申请日：2002-12-18

    Applicant: AJINOMOTO CO. INC

    Inventor： Mikiko Suga ,  Yoko Asakura ,  Masakazu Sugimoto ,  Hisao Ito

    IPC: C07H021/04 ,  C12N009/16

    CPC classification number: C12Y301/03003 ,  C12N9/16 ,  C12P13/06

    Abstract: The present invention provides a DNA coding for a protein defined in the following (A) or (B) is obtained from Brevibacterium flavum chromosomal DNA library by cloning a DNA fragment that complicates serB deficiency of Escherichia coli as a open reading frame in the DNA fragment. (A) A protein which comprises an amino acid sequence of SEQ ID: 2 in Sequence Listing; or (B) A protein which comprises an amino acid sequence including substitution, deletion, insertion, addition or inversion of one or several amino acids in the amino acid sequence of SEQ ID NO: 2 in Sequence Listing, and which has phosphoserine phosphatase activity.

    Method for producing L-glutamic acid by fermentation
    5.
    发明申请
    Method for producing L-glutamic acid by fermentation 失效
    通过发酵生产L-谷氨酸的方法

    公开(公告)号：US20030134397A1

    公开(公告)日：2003-07-17

    申请号：US10307320

    申请日：2002-12-02

    Applicant: AJINOMOTO CO. INC

    Inventor： Wataru Hibino ,  Yasuhiko Yoshihara ,  Masakazu Sugimoto ,  Harufumi Miwa

    IPC: C12P013/14 ,  C12N009/10 ,  C12N001/21

    CPC classification number: C12P13/14

    Abstract: A coryneform bacterium which has enhanced intracellular pyruvate carboxylase activity obtained by increasing copy number of a gene encoding the intracellular pyruvate carboxylase, or by enhancing function of a expression regulatory sequence for the gene, and has L-glutamic acid-producing ability is cultured in a medium so that L-glutamic acid should be produced and accumulated in the culture, and L-glutamic acid is collected from the culture.

    Abstract translation: 通过增加编码细胞内丙酮酸羧化酶的基因的拷贝数或通过增强基因的表达调节序列的功能而获得的具有增强的细胞内丙酮酸羧化酶活性并具有L-谷氨酸生产能力的棒状细菌被培养在 培养基中生成L-谷氨酸并在培养物中积累，从培养物中收集L-谷氨酸。

    Method of producing L-serine by fermentation
    6.
    发明申请
    Method of producing L-serine by fermentation 审中-公开
    通过发酵生产L-丝氨酸的方法

    公开(公告)号：US20030008358A1

    公开(公告)日：2003-01-09

    申请号：US10195548

    申请日：2002-07-16

    Applicant: AJINOMOTO CO. INC

    Inventor： Mikiko Suga ,  Masakazu Sugimoto ,  Tsuyoshi Osumi ,  Tsuyoshi Nakamatsu ,  Wataru Hibino ,  Mika Ito

    IPC: C12P013/06 ,  C12N009/04 ,  C07H021/04 ,  C12P021/02 ,  C12N001/21 ,  C12N015/74

    CPC classification number: C12N9/0006 ,  C12P13/06 ,  C12R1/13 ,  C12R1/15 ,  C12R1/19

    Abstract: Disclosed is a coryneform bacterium having resistance to azaserine or null-(2-thienyl)-DL-alanine and having L-serine productivity. Also, disclosed are D-3-phosphoglycerate dehydrogenase derived from a coryneform bacterium, in which feedback inhibition by L-serine is desensitized; the D-3-phosphoglycerate dehydrogenase, which is obtainable from a coryneform bacterium having resistance to azaserine or null-(2-thienyl)-DL-alanine and having L-serine productivity; the D-3-phosphoglycerate dehydrogenase having an amino acid sequence depicted in SEQ ID NO: 12 in Sequence Listing or the sequence including substitution, addition or deletion of one or more amino acids, wherein an amino acid residue corresponding to the 325th glutamic acid residue of the amino acid sequence in the SEQ ID NO: 12 is replaced with an amino acid other than glutamic acid; the D-3-phosphoglycerate dehydrogenase that has an amino acid sequence depicted in SEQ ID NO: 11 in Sequence Listing; a DNA coding for the D-3-phosphoglycerate dehydrogenase described above; the DNA that has a base sequence depicted in SEQ ID NO: 13 in Sequence Listing; a coryneform bacterium which harbors a recombinant DNA containing the DNA; and a method of producing L-serine, comprising the steps of cultivating the bacterium described above in a medium to allow accumulation of L-serine in the medium, and collecting the L-serine from the medium.

    Abstract translation: 公开了具有对丝氨酸或β-（2-噻吩基）-DL-丙氨酸具有L-丝氨酸生产力的抗性的棒状细菌。 此外，公开了衍生自棒状细菌的D-3-磷酸甘油酸脱氢酶，其中L-丝氨酸的反馈抑制被脱敏; D-3-磷酸甘油酸脱氢酶，其可从具有对β-丝氨酸或β-（2-噻吩基）-DL-丙氨酸具有抗丝氨酸生产力的棒状细菌获得; 具有序列表中SEQ ID NO：12所示氨基酸序列的D-3-磷酸甘油酸脱氢酶或包含一个或多个氨基酸的取代，添加或缺失的序列，其中对应于第325位谷氨酸残基的氨基酸残基 SEQ ID NO：12中的氨基酸序列被除了谷氨酸以外的氨基酸替换; 具有序列表中SEQ ID NO：11所示的氨基酸序列的D-3-磷酸甘油酸脱氢酶; 编码上述D-3-磷酸甘油酸脱氢酶的DNA; 具有序列表中SEQ ID NO：13所示碱基序列的DNA; 含有含DNA的重组DNA的棒状细菌; 以及生产L-丝氨酸的方法，包括以下步骤：在培养基中培养上述细菌以允许L-丝氨酸积累在培养基中，并从培养基中收集L-丝氨酸。

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