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8 条记录 (耗时 0.019 秒)

    Modified Leucine Dehydrogenase
    1.
    发明申请
    Modified Leucine Dehydrogenase 审中-公开

    公开(公告)号：US20160362662A1

    公开(公告)日：2016-12-15

    申请号：US15250056

    申请日：2016-08-29

    申请人： AJINOMOTO CO., INC.

    发明人： Wataru Hoshino ,  Yuya Kodama ,  Toshimi Mizukoshi ,  Uno Tagami

    IPC分类号： C12N9/06 ,  C12P13/08 ,  C12P13/06

    CPC分类号： C12N9/0016 ,  C07K2319/02 ,  C12P13/06 ,  C12P13/08 ,  C12Q1/32 ,  C12Y104/01009 ,  G01N33/6812 ,  G01N2333/90616

    摘要： The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.

    Modified Leucine Dehydrogenase
    2.
    发明申请
    Modified Leucine Dehydrogenase 有权
    改性亮氨酸脱氢酶

    公开(公告)号：US20150010936A1

    公开(公告)日：2015-01-08

    申请号：US14491501

    申请日：2014-09-19

    申请人： AJINOMOTO CO., INC.

    发明人： Wataru Hoshino ,  Yuya Kodama ,  Toshimi Mizukoshi ,  Uno Tagami

    IPC分类号： C12N9/06 ,  C12Q1/32

    CPC分类号： C12N9/0016 ,  C07K2319/02 ,  C12P13/06 ,  C12P13/08 ,  C12Q1/32 ,  C12Y104/01009 ,  G01N33/6812 ,  G01N2333/90616

    摘要： The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.

    摘要翻译： 本发明提供了用于测量总支链氨基酸浓度的方法和方法。 具体而言，本发明提供了一种修饰的酶，其中至少一个氨基酸残基被突变以提高与全部支链氨基酸的测量相关的亮氨酸脱氢酶的性质，例如， 亮氨酸脱氢酶对总支链氨基酸的底物特异性，亮氨酸脱氢酶对任何支链氨基酸的活性和亮氨酸脱氢酶的热稳定性; 以及分析总支链氨基酸的方法，包括使用修饰的酶测量测试样品中所含的总支链氨基酸。

    Modified Leucine Dehydrogenase
    3.
    发明申请
    Modified Leucine Dehydrogenase 有权

    公开(公告)号：US20160222359A1

    公开(公告)日：2016-08-04

    申请号：US15133599

    申请日：2016-04-20

    申请人： AJINOMOTO CO., INC.

    发明人： Wataru Hoshino ,  Yuya Kodama ,  Toshimi Mizukoshi ,  Uno Tagami

    IPC分类号： C12N9/06 ,  C12P13/06 ,  C12Q1/32

    CPC分类号： C12N9/0016 ,  C07K2319/02 ,  C12P13/06 ,  C12P13/08 ,  C12Q1/32 ,  C12Y104/01009 ,  G01N33/6812 ,  G01N2333/90616

    摘要： The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.

    Modified leucine dehydrogenase
    5.
    发明授权
    Modified leucine dehydrogenase 有权
    改性亮氨酸脱氢酶

    公开(公告)号：US09453206B2

    公开(公告)日：2016-09-27

    申请号：US15133599

    申请日：2016-04-20

    申请人： AJINOMOTO CO., INC.

    发明人： Wataru Hoshino ,  Yuya Kodama ,  Toshimi Mizukoshi ,  Uno Tagami

    IPC分类号： C12N9/06 ,  C12P41/00 ,  C12Q1/32 ,  G01N33/68 ,  C12P13/06

    CPC分类号： C12N9/0016 ,  C07K2319/02 ,  C12P13/06 ,  C12P13/08 ,  C12Q1/32 ,  C12Y104/01009 ,  G01N33/6812 ,  G01N2333/90616

    摘要： The present invention provides a means and method useful for measurement of a total branched-chain amino acid concentration. Specifically, the present invention provides a modified enzyme in which at least one amino acid residue is mutated so as to improve a property of a leucine dehydrogenase which is associated with the measurement of the total branched-chain amino acids, such as, for example, substrate specificities of leucine dehydrogenase for total branched-chain amino acids, activity of leucine dehydrogenase for any branched-chain amino acids, and thermal stability of leucine dehydrogenase; and a method of analyzing the total branched-chain amino acids, comprising measuring the total branched-chain amino acids contained in a test sample using the modified enzyme.

    摘要翻译： 本发明提供了用于测量总支链氨基酸浓度的方法和方法。 具体而言，本发明提供了一种修饰的酶，其中至少一个氨基酸残基被突变以提高与全部支链氨基酸的测量相关的亮氨酸脱氢酶的性质，例如， 亮氨酸脱氢酶对总支链氨基酸的底物特异性，亮氨酸脱氢酶对任何支链氨基酸的活性和亮氨酸脱氢酶的热稳定性; 以及分析总支链氨基酸的方法，包括使用修饰的酶测量测试样品中所含的总支链氨基酸。