公开(公告)号：US09945762B2

公开(公告)日：2018-04-17

申请号：US14586882

申请日：2014-12-30

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Edwin E. Wikfors ,  Brian A. Bidlingmeyer ,  Klaus Witt

IPC: G01N30/06 ,  G01N1/28 ,  G01N30/20 ,  G01N30/36 ,  G01N30/34

CPC classification number: G01N1/28 ,  G01N30/20 ,  G01N30/36 ,  G01N2030/202 ,  G01N2030/207 ,  G01N2030/342

Abstract: A method and a system for introducing a sample into a mobile phase of a chromatography system is provided. The method includes initially directing the mobile phase directly into a separation unit of the chromatography system, bypassing a sample loop, the mobile phase including a combined solvent, metered from a pressurized first solvent and a second solvent; loading the sample into the sample loop, while the mobile phase continues to be directed directly into the separation unit; pressurizing the sample in the sample loop with the pressurized first solvent, while the mobile phase continues to be directed directly into the separation unit; and switching the sample loop into the mobile phase, thereby introducing the pressurized sample to the separation unit.

公开(公告)号：US20160187304A1

公开(公告)日：2016-06-30

申请号：US14586882

申请日：2014-12-30

Applicant: AGILENT TECHNOLOGIES, INC.

Inventor： Edwin E. Wikfors ,  Brian A. Bidlingmeyer ,  Klaus Witt

IPC: G01N30/06 ,  G01N1/28

CPC classification number: G01N1/28 ,  G01N30/20 ,  G01N30/36 ,  G01N2030/202 ,  G01N2030/207 ,  G01N2030/342

Abstract: A method and a system for introducing a sample into a mobile phase of a chromatography system is provided. The method includes initially directing the mobile phase directly into a separation unit of the chromatography system, bypassing a sample loop, the mobile phase including a combined solvent, metered from a pressurized first solvent and a second solvent; loading the sample into the sample loop, while the mobile phase continues to be directed directly into the separation unit; pressurizing the sample in the sample loop with the pressurized first solvent, while the mobile phase continues to be directed directly into the separation unit; and switching the sample loop into the mobile phase, thereby introducing the pressurized sample to the separation unit.

Abstract translation: 提供了一种用于将样品引入色谱系统的流动相的方法和系统。 该方法包括首先将流动相直接引入色谱系统的分离单元，绕过样品环，流动相包括从加压的第一溶剂和第二溶剂计量的组合溶剂; 将样品加载到样品回路中，同时流动相继续直接导入分离单元; 在加压的第一溶剂中对样品环中的样品加压，同时流动相继续被直接引导到分离单元中; 并将样品环切换到流动相中，从而将加压的样品引入分离单元。