公开(公告)号：US20220089943A1

公开(公告)日：2022-03-24

申请号：US17542223

申请日：2021-12-03

Applicant: AAT Bioquest, Inc.

Inventor： Zhenjun Diwu ,  Haitao Guo ,  Jixiang Liu ,  Qin Zhao ,  Ruogu Peng

IPC: C09K11/06 ,  G01N21/64 ,  G01N33/533 ,  C08G61/12

Abstract: Fluorescent water soluble conjugated polymers including polycyclic aromatic comonomers are provided. The conjugated polymers can be linked to an acceptor fluorescent dye. The conjugated polymers find use in conjugates with biological substrates having applications in a variety of applications including methods of analyte detection.

公开(公告)号：US09810700B1

公开(公告)日：2017-11-07

申请号：US15610184

申请日：2017-05-31

Applicant: AAT Bioquest, Inc.

Inventor： Zhenjun Diwu ,  Haitao Guo ,  Ruogu Peng ,  Qin Zhao ,  Jinfang Liao ,  Feng Liu

IPC: C07D311/90 ,  G01N33/84 ,  C07D493/10 ,  C07C229/22 ,  C07F5/02 ,  C07D307/94 ,  G01N33/58

CPC classification number: G01N33/84 ,  C07C229/22 ,  C07D307/94 ,  C07D311/90 ,  C07D493/10 ,  C07F5/022 ,  C09B11/24 ,  C09B11/28 ,  C09B15/00 ,  C09B23/04 ,  C09B69/008 ,  G01N33/582

Abstract: The present disclosure provides fluorogenic compounds useful for preparing fluorescent calcium ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators in methods of detection, discrimination and quantification of metal ions. The subject fluorogenic compounds and fluorescent ion indicators can include a chelating group based on a 2-aminophenoxyethylene glycol 2-aminoethyl ether, N,N,N′,N′-tetraacetic acid (PEGTA) moiety or precursor thereof where the phenyl group of the PEGTA is substituted with or fused with a fluorophore moiety of interest. The subject methods find use in the detection of intracellular calcium ions. Also provided are kits for use in practicing the subject methods.

公开(公告)号：US12169166B2

公开(公告)日：2024-12-17

申请号：US17176847

申请日：2021-02-16

Applicant: AAT Bioquest, Inc.

Inventor： Zhenjun Diwu ,  Haitao Guo ,  Lok N. Neupane ,  Deven Patel

IPC: G01N1/30 ,  C07D417/06 ,  C09B23/01 ,  C09B23/10 ,  G01N21/64

Abstract: This disclosure provides cyanine dye compounds having linked hydrogen bond-forming groups that can be used for detecting nucleic acids, particularly for fluorescent staining of DNA, in a biological sample. We found that the simultaneous incorporation of at least two hydrogen bond-forming groups (HBGs) into such a nucleic acid stain can provide for crosslinking interactions between the stain and the target nucleic acid, and lead to a significant increase in the detection sensitivity without substantially increasing undesirable cytotoxicity or mutagenicity properties of the dye compounds. The cyanine dye compounds can have a cyanine structure that connects two particular fused heterocycle ring systems, where at least two HBGs are connected to the core structure through linkers. Also provided are nucleic acid complexes including the cyanine dye compounds, and methods of using the compounds to detect nucleic acids in a biological sample.

公开(公告)号：US20200263084A1

公开(公告)日：2020-08-20

申请号：US16592464

申请日：2019-10-03

Applicant: AAT Bioquest, Inc.

Inventor： Zhenjun Diwu ,  Haitao Guo ,  Jixiang Liu ,  Qin Zhao ,  Ruogu Peng

IPC: C09K11/06 ,  G01N21/64 ,  G01N33/533 ,  C08G61/12

Abstract: Fluorescent water soluble conjugated polymers including polycyclic aromatic comonomers are provided. The conjugated polymers can be linked to an acceptor fluorescent dye. The conjugated polymers find use in conjugates with biological substrates having applications in a variety of applications including methods of analyte detection.

公开(公告)号：US10239853B1

公开(公告)日：2019-03-26

申请号：US15998019

申请日：2018-06-12

Applicant: AAT BIOQUEST, INC.

Inventor： Zhenjun Diwu ,  Ruogu Peng ,  Haitao Guo ,  Zhen Luo

IPC: C07D307/77 ,  G01N33/58

Abstract: The present invention is generally directed to fluorescent dyes useful for preparing fluorescent oligonucleotides, and the use of the fluorescent oligonucleotides for the detection, discrimination and quantification of molecular biology targets.

公开(公告)号：US20180171070A1

公开(公告)日：2018-06-21

申请号：US15860965

申请日：2018-01-03

Applicant: AAT Bioquest, Inc. ,  Affymetrix, Inc.

Inventor： Zhenjun Diwu ,  Haitao Guo ,  Ruogu Peng ,  Qin Zhao ,  Travis Jennings ,  Castle Funatake

IPC: C08G61/12 ,  C09K11/06 ,  G01N33/533

CPC classification number: C08G61/128 ,  C08G61/12 ,  C08G2261/12 ,  C08G2261/1424 ,  C08G2261/143 ,  C08G2261/3142 ,  C08G2261/3242 ,  C08G2261/344 ,  C08G2261/411 ,  C08G2261/522 ,  C08G2261/72 ,  C08G2261/77 ,  C08G2261/94 ,  C09K11/06 ,  C09K2211/1416 ,  C09K2211/145 ,  G01N33/533

Abstract: The invention provides for polyfluoreno[4,5-cde]oxepine conjugates and their use in methods of analyte detection.

公开(公告)号：US11827824B2

公开(公告)日：2023-11-28

申请号：US17542223

申请日：2021-12-03

Applicant: AAT Bioquest, Inc.

Inventor： Zhenjun Diwu ,  Haitao Guo ,  Jixiang Liu ,  Qin Zhao ,  Ruogu Peng

IPC: C09K11/06 ,  G01N21/64 ,  G01N33/533 ,  C08G61/12

CPC classification number: C09K11/06 ,  C08G61/124 ,  G01N21/6428 ,  G01N33/533 ,  C08G2261/3241 ,  C09K2211/1466 ,  G01N2021/6439

Abstract: Fluorescent water soluble conjugated polymers including polycyclic aromatic comonomers are provided. The conjugated polymers can be linked to an acceptor fluorescent dye. The conjugated polymers find use in conjugates with biological substrates having applications in a variety of applications including methods of analyte detection.

公开(公告)号：US20230131000A1

公开(公告)日：2023-04-27

申请号：US17793173

申请日：2021-01-28

Applicant: AAT Bioquest, Inc. ,  Affymetrix, Inc.

Inventor： Zhenjun Diwu ,  Haitao Guo ,  Ruogu Peng ,  Travis Jennings

IPC: G01N33/533 ,  C07K17/08 ,  C08G73/06

Abstract: The invention provides for UV excitable polyfluorene based conjugates and their use in methods of analyte detection.

公开(公告)号：US11237170B2

公开(公告)日：2022-02-01

申请号：US16350574

申请日：2018-12-04

Applicant: AAT BIOQUEST, INC.

Inventor： Zhenjun Diwu ,  Zhen Luo ,  Jixiang Liu ,  Pengfei Dong

IPC: G01N33/58 ,  C07D333/04 ,  G01N33/532

Abstract: Styryl compounds useful for preparing biological detections probes, and the use of the Styryl compounds for the detection, discrimination and quantification of biological targets.

公开(公告)号：US20200174006A1

公开(公告)日：2020-06-04

申请号：US16350574

申请日：2018-12-04

Applicant: AAT BIOQUEST, INC.

Inventor： Zhenjun Diwu ,  Zhen Luo ,  Jixiang Liu ,  Pengfei Dong

IPC: G01N33/58 ,  G01N33/532 ,  C07D333/04

Abstract: Styryl compounds useful for preparing biological detections probes, and the use of the Styryl compounds for the detection, discrimination and quantification of biological targets.