公开(公告)号：US10738304B2

公开(公告)日：2020-08-11

申请号：US12808504

申请日：2008-12-18

申请人： Takanori Kubo ,  Hideki Ohba ,  Kazuo Sakurai ,  Jusaku Minari ,  Atsushi Uno

发明人： Takanori Kubo ,  Hideki Ohba ,  Kazuo Sakurai ,  Jusaku Minari ,  Atsushi Uno

IPC分类号： C12N5/00 ,  C12N15/11 ,  C07H21/02

摘要： An object of the present invention is to provide novel double-stranded RNA having an RNA interference effect, in which the cellular uptake and the resistance to enzymatic degradation are improved, without reducing the RNA interference effect.The cellular uptake and the resistance to enzymatic degradation of a complex of a polysaccharide having a β-1,3-glucan skeleton and double-stranded RNA can be significantly improved while maintaining the RNA interference effect, by fulfilling the following conditions (i) to (iii): (i) the double-stranded RNA has a sense strand consisting of a base sequence complementary to a target sequence in a target gene and an antisense strand containing a base sequence complementary to the sense strand, and the double-stranded RNA can inhibit expression of the target gene; (ii) the double-stranded RNA has a single-stranded polydeoxyadenine bound directly or via a linker to the end of at least one of the sense strand and the antisense strand; and (iii) the polysaccharide and the single-stranded polydeoxyadenine form a complex.

公开(公告)号：US09040492B2

公开(公告)日：2015-05-26

申请号：US12935546

申请日：2009-03-31

申请人： Takanori Kubo ,  Hideki Ohba ,  Hidekazu Toyobuku ,  Hirotake Hayashi

发明人： Takanori Kubo ,  Hideki Ohba ,  Hidekazu Toyobuku ,  Hirotake Hayashi

IPC分类号： A01N43/04 ,  C12N15/11

CPC分类号： C12N15/111 ,  C12N2310/3515 ,  C12N2320/32

摘要： An object of the present invention is to provide a novel double-stranded RNA that has high resistance to nuclease and cellular uptake efficiency and that can produce an excellent RNA interference effect. The present invention provides a double-stranded lipid-modified RNA comprising an antisense strand having a nucleotide sequence complementary to a target sequence in a target gene, and a sense strand having a nucleotide sequence complementary to the antisense strand, the double-stranded RNA being capable of suppressing the expression of the target gene, and the sense strand having a double-stranded lipid bound directly or via a linker to at least one of the first to sixth nucleotides from the 5′ end.

摘要翻译： 本发明的目的是提供对核酸酶和细胞摄取效率具有高抗性并且可以产生优异的RNA干扰效果的新型双链RNA。 本发明提供了包含具有与靶基因中的靶序列互补的核苷酸序列的反义链和具有与反义链互补的核苷酸序列的有义链的双链脂质修饰的RNA，所述双链RNA为 能够抑制靶基因的表达，并且将具有直链或经由连接基结合的双链脂质的有义链与5'末端的至少一个第一至第六个核苷酸结合。

公开(公告)号：US08981074B2

公开(公告)日：2015-03-17

申请号：US12738797

申请日：2008-10-24

申请人： Takanori Kubo ,  Hideki Ohba ,  Hidekazu Toyobuku ,  Hirotake Hayashi

发明人： Takanori Kubo ,  Hideki Ohba ,  Hidekazu Toyobuku ,  Hirotake Hayashi

IPC分类号： C07H21/04 ,  A61K47/48

CPC分类号： A61K47/48038 ,  A61K47/542 ,  A61K47/543

摘要： An object of the present invention is to provide a novel double-stranded RNA that has high nuclease resistance and high cellular uptake efficiency, and that is capable of producing an excellent RNA interference effect. The present invention provides a lipid-modified double-stranded RNA comprising a sense strand having a nucleotide sequence complementary to a target sequence, and an antisense strand having a nucleotide sequence complementary to the sense strand, the double-stranded RNA being capable of inhibiting the expression of the target gene, the sense strand having a lipid linked to at least one of the first to sixth nucleotides from the 5′ end side directly or via a linker.

摘要翻译： 本发明的目的是提供具有高核酸酶抗性和高细胞摄取效率的新型双链RNA，并能够产生优异的RNA干扰效果。 本发明提供脂质修饰的双链RNA，其包含具有与靶序列互补的核苷酸序列的有义链和具有与有义链互补的核苷酸序列的反义链，所述双链RNA能够抑制 靶基因的表达，有义链具有从5'末端侧的至少一个第一至第六个核苷酸直接或通过连接体连接的脂质。

公开(公告)号：US20110111501A1

公开(公告)日：2011-05-12

申请号：US12808504

申请日：2008-12-18

申请人： Takanori Kubo ,  Hideki Ohba ,  Kazuo Sakurai ,  Jusaku Minari ,  Atsushi Uno

发明人： Takanori Kubo ,  Hideki Ohba ,  Kazuo Sakurai ,  Jusaku Minari ,  Atsushi Uno

IPC分类号： C12N5/00 ,  C07H21/02

CPC分类号： C12N15/111 ,  C12N2310/14 ,  C12N2310/3519 ,  C12N2320/32 ,  C12N2320/51

摘要： An object of the present invention is to provide novel double-stranded RNA having an RNA interference effect, in which the cellular uptake and the resistance to enzymatic degradation are improved, without reducing the RNA interference effect.The cellular uptake and the resistance to enzymatic degradation of a complex of a polysaccharide having a β-1,3-glucan skeleton and double-stranded RNA can be significantly improved while maintaining the RNA interference effect, by fulfilling the following conditions (i) to (iii): (i) the double-stranded RNA has a sense strand consisting of a base sequence complementary to a target sequence in a target gene and an antisense strand containing a base sequence complementary to the sense strand, and the double-stranded RNA can inhibit expression of the target gene; (ii) the double-stranded RNA has a single-stranded polydeoxyadenine bound directly or via a linker to the end of at least one of the sense strand and the antisense strand; and (iii) the polysaccharide and the single-stranded polydeoxyadenine form a complex.

摘要翻译： 本发明的目的是提供具有RNA干扰效应的新颖的双链RNA，其中细胞摄取和对酶降解的抗性得到改善，而不降低RNA干扰效应。 通过满足以下条件，可以显着改善具有β-葡聚糖骨架和双链RNA的多糖的复合物的细胞摄取和抗降解，同时保持RNA干扰效应（i） 至（iii）：（i）双链RNA具有由与靶基因中的靶序列互补的碱基序列的有义链和含有与有义链互补的碱基序列的反义链，双链 RNA可抑制靶基因的表达; （ii）双链RNA具有单链多脱氧腺嘌呤，其直接​​或经由接头连接至至少一个有义链和反义链的末端; 和（iii）多糖和单链多脱氧腺嘌呤形成复合物。

公开(公告)号：US20100013577A1

公开(公告)日：2010-01-21

申请号：US12567693

申请日：2009-09-25

申请人： Takanori KUBO ,  Hiromichi Yoshikawa

发明人： Takanori KUBO ,  Hiromichi Yoshikawa

IPC分类号： H01P3/08

CPC分类号： H01P1/20327 ,  H01P1/20354

摘要： A bandpass filter for a wide frequency band such as UWB is disclosed. The bandpass filter can receive a pair of signals, namely a balanced signal, and output a pair of signals. The bandpass filter comprises a plurality of ½ wavelength resonance electrodes, a plurality of ¼ wavelength resonance electrodes and a plurality of coupling electrodes. A transmission characteristic of the bandpass filter having flat and low loss over the entire region of the broad pass band can be achieved.

摘要翻译： 公开了一种用于诸如UWB的宽频带的带通滤波器。 带通滤波器可以接收一对信号，即平衡信号，并输出一对信号。 带通滤波器包括多个1/2波长谐振电极，多个1/4波长谐振电极和多个耦合电极。 可以实现在宽带的整个区域上具有平坦且低损耗的带通滤波器的传输特性。

公开(公告)号：US20100298411A1

公开(公告)日：2010-11-25

申请号：US12738797

申请日：2008-10-24

申请人： Takanori Kubo ,  Hideki Ohba ,  Hidekazu Toyobuku ,  Hirotake Hayashi

发明人： Takanori Kubo ,  Hideki Ohba ,  Hidekazu Toyobuku ,  Hirotake Hayashi

IPC分类号： A61K31/713 ,  C07H21/02 ,  C12N15/113 ,  A61P5/00

CPC分类号： A61K47/48038 ,  A61K47/542 ,  A61K47/543

摘要： An object of the present invention is to provide a novel double-stranded RNA that has high nuclease resistance and high cellular uptake efficiency, and that is capable of producing an excellent RNA interference effect. The present invention provides a lipid-modified double-stranded RNA comprising a sense strand having a nucleotide sequence complementary to a target sequence, and an antisense strand having a nucleotide sequence complementary to the sense strand, the double-stranded RNA being capable of inhibiting the expression of the target gene, the sense strand having a lipid linked to at least one of the first to sixth nucleotides from the 5′ end side directly or via a linker.

摘要翻译： 本发明的目的是提供具有高核酸酶抗性和高细胞摄取效率的新型双链RNA，并能够产生优异的RNA干扰效果。 本发明提供脂质修饰的双链RNA，其包含具有与靶序列互补的核苷酸序列的有义链和具有与有义链互补的核苷酸序列的反义链，所述双链RNA能够抑制 靶基因的表达，有义链具有从5'末端侧的至少一个第一至第六个核苷酸直接或通过连接体连接的脂质。

公开(公告)号：US08497748B2

公开(公告)日：2013-07-30

申请号：US12580944

申请日：2009-10-16

申请人： Takanori Kubo ,  Hiromichi Yoshikawa

发明人： Takanori Kubo ,  Hiromichi Yoshikawa

IPC分类号： H01P1/203

CPC分类号： H01P1/20345

摘要： A bandpass filter for a wide frequency band such as UWB is disclosed. The bandpass filter can receive a pair of signals, namely a differential signal, and output a single signal, namely an unbalanced signal. A transmission characteristic of the bandpass filter having an attenuation pole near both sides of the passband can be achieved.

摘要翻译： 公开了一种用于诸如UWB的宽频带的带通滤波器。 带通滤波器可以接收一对信号，即差分信号，并输出单个信号，即不平衡信号。 可以实现具有靠近通带两侧的衰减极的带通滤波器的传输特性。

公开(公告)号：US20110034545A1

公开(公告)日：2011-02-10

申请号：US12935546

申请日：2009-03-31

申请人： Takanori Kubo ,  Hideki Ohba ,  Hidekazu Toyobuku ,  Hirotake Hayashi

发明人： Takanori Kubo ,  Hideki Ohba ,  Hidekazu Toyobuku ,  Hirotake Hayashi

IPC分类号： A61K31/713 ,  C07H21/02 ,  C12N5/071 ,  A61P35/00 ,  A61P31/18

CPC分类号： C12N15/111 ,  C12N2310/3515 ,  C12N2320/32

摘要： An object of the present invention is to provide a novel double-stranded RNA that has high resistance to nuclease and cellular uptake efficiency and that can produce an excellent RNA interference effect. The present invention provides a double-stranded lipid-modified RNA comprising a sense strand having a nucleotide sequence complementary to a target sequence in a target gene, and an antisense strand having a nucleotide sequence complementary to the sense strand, the double-stranded RNA being capable of suppressing the expression of the target gene, and the sense strand having a double-stranded lipid bound directly or via a linker to at least one of the first to sixth nucleotides from the 5′ end.

摘要翻译： 本发明的目的是提供对核酸酶和细胞摄取效率具有高抗性并且可以产生优异的RNA干扰效果的新型双链RNA。 本发明提供了包含具有与靶基因中的靶序列互补的核苷酸序列的有义链和具有与有义链互补的核苷酸序列的反义链的双链脂质修饰的RNA，所述双链RNA为 能够抑制靶基因的表达，并且将具有直链或经由连接基结合的双链脂质的有义链与5'末端的至少一个第一至第六个核苷酸结合。

公开(公告)号：US06356173B1

公开(公告)日：2002-03-12

申请号：US09322739

申请日：1999-05-28

申请人： Koichi Nagata ,  Kenji Kitazawa ,  Shinichi Koriyama ,  Shigeki Morioka ,  Takanori Kubo ,  Hidehiro Minamiue ,  Masanobu Ishida ,  Akira Nakayama ,  Naoyuki Shino

发明人： Koichi Nagata ,  Kenji Kitazawa ,  Shinichi Koriyama ,  Shigeki Morioka ,  Takanori Kubo ,  Hidehiro Minamiue ,  Masanobu Ishida ,  Akira Nakayama ,  Naoyuki Shino

IPC分类号： H01P502

CPC分类号： H05K1/0239 ,  H01L23/66 ,  H01L2223/6627 ,  H01L2924/0002 ,  H01L2924/09701 ,  H01L2924/15192 ,  H01L2924/16152 ,  H01L2924/16153 ,  H01L2924/16251 ,  H01L2924/1903 ,  H01L2924/19107 ,  H01L2924/3011 ,  H05K1/0243 ,  H05K1/0272 ,  H05K1/0298 ,  H05K3/0061 ,  H05K2201/09054 ,  H01L2924/00

摘要： A high-frequency module in which a plurality of cavities are formed on the surface of a dielectric board, internal high-frequency signal transmission lines are connected to high-frequency devices in the cavities and are located in the cavities, the internal high-frequency signal transmission lines being electromagnetically coupled to the external high-frequency signal transmission line, and the high-frequency devices in the cavities are connected to each other relying on the electromagnetic coupling through the external line. The high-frequency module enables high-frequency signals to be transmitted among the high-frequency devices with a small loss. The module has a very simple structure, and is cheaply produced, and offers an advantage that it can be easily obtained in a small size.

摘要翻译： 一种高频模块，其中在介质板的表面上形成多个空腔，内部高频信号传输线连接到空腔中的高频器件，并且位于空腔中，内部高频 信号传输线被电磁耦合到外部高频信号传输线，并且空腔中的高频器件通过外部线路依赖于电磁耦合而相互连接。 高频模块使高频信号能够以较小的损耗在高频器件之间传输。 该模块具有非常简单的结构，并且便宜地生产，并且具有可以容易地以小尺寸获得的优点。

公开(公告)号：US5331297A

公开(公告)日：1994-07-19

申请号：US993124

申请日：1992-12-18

申请人： Takanori Kubo ,  Shigeo Tanahashi

发明人： Takanori Kubo ,  Shigeo Tanahashi

IPC分类号： H01P5/12

CPC分类号： H01P5/12 ,  Y10S505/865

摘要： An impedance-matching circuit assembly supplies a.c. power from an external a.c. source to Josephson devices. The assembly comprises resonant-line composed quarter-wave impedance conversion circuits, interconnected in a tree. The impedance-conversion circuits are assembled such that connected to the a.c. source is a circuit having a first-stage impedance, followed by two circuits having second-state impedances connected in parallel, to each of which are connected in parallel two circuits having third-stage impedances, each of which in turn is connected to supply input terminals of two of the Josephson devices.

摘要翻译： 阻抗匹配电路组件提供直流 外部电源的功率 来源于约瑟夫森设备。 该组件包括在树中互连的谐振线组成的四分之一波阻抗转换电路。 阻抗转换电路组装成连接到交流电源。 源极是具有第一级阻抗的电路，其后是具有并联连接的第二状态阻抗的两个电路，每个电路并联连接具有第三级阻抗的两个电路，每个电路依次连接到电源输入端 两个Josephson设备的终端。