公开(公告)号：US20150056666A1

公开(公告)日：2015-02-26

申请号：US14532259

申请日：2014-11-04

申请人： Alexander RETH ,  Brigitte Bathe ,  Stephan Hans ,  Wilfried Claes

发明人： Alexander RETH ,  Brigitte Bathe ,  Stephan Hans ,  Wilfried Claes

IPC分类号： C12N15/77 ,  C12P13/10 ,  C12P13/06 ,  C12P13/08

CPC分类号： C12P13/08 ,  C12N9/0008 ,  C12N15/77 ,  C12P13/04 ,  C12P13/06 ,  C12P13/10

摘要： The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.

摘要翻译： 本发明涉及具有启动子活性的分离的多核苷酸，编码甘油醛-3-磷酸脱氢酶的间隙基因的启动子的变体; 以及产生和/或分泌精细化学物质的微生物，所述微生物包括具有启动子活性的分离的多核苷酸，其使各种基因与特定的起始菌株相比过表达; 以及使用微生物制备精细化学品的方法。

公开(公告)号：US08912313B2

公开(公告)日：2014-12-16

申请号：US13534359

申请日：2012-06-27

申请人： Alexander Reth ,  Brigitte Bathe ,  Stephan Hans ,  Wilfried Claes

发明人： Alexander Reth ,  Brigitte Bathe ,  Stephan Hans ,  Wilfried Claes

IPC分类号： C12N15/11 ,  C12N15/77 ,  C12N9/02 ,  C12P13/08 ,  C12P13/10 ,  C12P13/04

CPC分类号： C12P13/08 ,  C12N9/0008 ,  C12N15/77 ,  C12P13/04 ,  C12P13/06 ,  C12P13/10

摘要： The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.

摘要翻译： 本发明涉及具有启动子活性的分离的多核苷酸，编码甘油醛-3-磷酸脱氢酶的间隙基因的启动子的变体; 以及产生和/或分泌精细化学物质的微生物，所述微生物包括具有启动子活性的分离的多核苷酸，其使各种基因与特定的起始菌株相比过表达; 以及使用微生物制备精细化学品的方法。

公开(公告)号：US07982020B2

公开(公告)日：2011-07-19

申请号：US11722301

申请日：2005-12-13

申请人： Stephan Hans ,  Brigitte Bathe ,  Georg Thierbach

发明人： Stephan Hans ,  Brigitte Bathe ,  Georg Thierbach

IPC分类号： C07H21/04

CPC分类号： C12N9/12

摘要： The invention relates to mutated variants of the proB gene from coryneform bacteria, which encode γ-glutamyl kinase, and to processes for fermentative production of L-proline using bacteria which contain this mutation.

摘要翻译： 本发明涉及编码γ-谷氨酰激酶的棒状细菌的proB基因的突变变体，以及使用含有该突变的细菌发酵生产L-脯氨酸的方法。

公开(公告)号：US07160703B2

公开(公告)日：2007-01-09

申请号：US10460294

申请日：2003-06-13

申请人： Bettina Moeckel ,  Stephan Hans ,  Natalie Schischka ,  Walter Pfefferle

发明人： Bettina Moeckel ,  Stephan Hans ,  Natalie Schischka ,  Walter Pfefferle

IPC分类号： C12P13/04 ,  C12P13/24 ,  C12P13/22 ,  C12P13/20 ,  C12P13/14 ,  C12P13/18 ,  C12P13/12 ,  C12N1/21 ,  C12N9/12 ,  C07H21/04

CPC分类号： C12N9/12 ,  C12P13/04

摘要： The present invention provides nucleotide sequences from Coryneform bacteria which code for the PtsI protein and a process for the fermentative preparation of amino acids using bacteria in which the ptsI gene is enhanced.

摘要翻译： 本发明提供了编码PtsI蛋白的棒状细菌的核苷酸序列，以及使用其中ptsI基因增强的细菌发酵制备氨基酸的方法。

公开(公告)号：US07105302B2

公开(公告)日：2006-09-12

申请号：US10940606

申请日：2004-09-15

申请人： Brigitte Bathe ,  Stephan Hans ,  Mike Farwick ,  Thomas Hermann

发明人： Brigitte Bathe ,  Stephan Hans ,  Mike Farwick ,  Thomas Hermann

IPC分类号： C12Q1/68 ,  C12N9/12 ,  C12N1/20 ,  C12N15/09 ,  C07H21/04

CPC分类号： C12N9/1205 ,  C12P13/04 ,  C12Y207/01037

摘要： An isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: (a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID No. 2, (b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, (c) a polynucleotide which is complementary to the polynucleotides of (a) or (b), and (d) a polynucleotide containing at least 15 consecutive nucleotides of the polynucleotide sequence of (a), (b) or (c), and a fermentation process for the preparation of L-amino acids using corynebacteria in which at least the pknB gene is amplified, and to the use, as hybridization probes, of polynucleotides containing the sequences according to the invention.

公开(公告)号：US20050074791A1

公开(公告)日：2005-04-07

申请号：US10804120

申请日：2004-03-19

申请人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

发明人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfefferle ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC分类号： C07K14/34 ,  C12N1/21 ,  C12N15/52 ,  C12P13/08 ,  C12Q1/68

CPC分类号： C12N15/52 ,  C07K14/34 ,  C12P13/08

摘要： The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

摘要翻译： 本发明涉及具有增强的lysE基因（赖氨酸出口载体基因）的棒状杆菌L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被增强，特别是过度表达，并且涉及制备L- 赖氨酸。

公开(公告)号：US06844176B1

公开(公告)日：2005-01-18

申请号：US10270512

申请日：2002-10-16

申请人： Brigitte Bathe ,  Stephan Hans

发明人： Brigitte Bathe ,  Stephan Hans

IPC分类号： C07H21/04 ,  C12N9/12 ,  C12N9/10

CPC分类号： C12N9/12 ,  C07H21/04

摘要： Alleles of the lysC gene from corynebacteria that code for desensitized aspartokinases, and to processes for the preparation of L-lysine using bacteria containing these alleles.

摘要翻译： 来自编码脱敏天冬氨酸激酶的棒状杆菌的lysC基因的等位基因，以及使用含有这些等位基因的细菌制备L-赖氨酸的方法。

公开(公告)号：US06200785B1

公开(公告)日：2001-03-13

申请号：US09353133

申请日：1999-07-14

申请人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfeffere ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

发明人： Caroline Kreutzer ,  Stephan Hans ,  Mechthild Rieping ,  Bettina Mockel ,  Walter Pfeffere ,  Lothar Eggeling ,  Hermann Sahm ,  Miroslav Patek

IPC分类号： C12P1308

CPC分类号： C07K14/34 ,  C12N15/52 ,  C12P13/08

摘要： The invention relates to L-lysine-producing strains of corynebacteria with amplified lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are amplified and, in particular, overexpressed, and to a process for the preparation of L-lysine.

摘要翻译： 本发明涉及具有扩增的lysE基因（赖氨酸出口载体基因）的棒状杆菌的L-赖氨酸生产菌株，其中选自包含dapA基因（二氢吡啶二酸合成酶基因），lysC基因（天冬氨酸激酶基因） ，dapB基因（二氢吡啶二羧酸还原酶基因）和pyc基因，特别是dapA基因和lysC基因（天冬氨酸激酶基因）被扩增，特别是过表达，以及制备L-赖氨酸的方法。

公开(公告)号：US09074229B2

公开(公告)日：2015-07-07

申请号：US14607177

申请日：2015-01-28

申请人： Alexander Reth ,  Brigitte Bathe ,  Stephan Hans ,  Wilfried Claes

发明人： Alexander Reth ,  Brigitte Bathe ,  Stephan Hans ,  Wilfried Claes

IPC分类号： C12P13/06 ,  C12P13/08 ,  C12P13/04 ,  C12P13/10 ,  C12N15/77 ,  C12N9/02

CPC分类号： C12P13/08 ,  C12N9/0008 ,  C12N15/77 ,  C12P13/04 ,  C12P13/06 ,  C12P13/10

摘要： The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.

摘要翻译： 本发明涉及具有启动子活性的分离的多核苷酸，编码甘油醛-3-磷酸脱氢酶的间隙基因的启动子的变体; 以及产生和/或分泌精细化学物质的微生物，所述微生物包括具有启动子活性的分离的多核苷酸，其使各种基因与特定的起始菌株相比过表达; 以及使用微生物制备精细化学品的方法。

公开(公告)号：US20150140614A1

公开(公告)日：2015-05-21

申请号：US14607177

申请日：2015-01-28

申请人： ALEXANDER RETH ,  BRIGITTE BATHE ,  STEPHAN HANS ,  WILFRIED CLAES

发明人： ALEXANDER RETH ,  BRIGITTE BATHE ,  STEPHAN HANS ,  WILFRIED CLAES

IPC分类号： C12P13/08 ,  C12N9/02 ,  C12N15/77 ,  C12P13/04 ,  C12P13/10

CPC分类号： C12P13/08 ,  C12N9/0008 ,  C12N15/77 ,  C12P13/04 ,  C12P13/06 ,  C12P13/10

摘要： The invention relates to an isolated polynucleotide having promoter activity, a variant of the promoter of the gap gene coding for glyceraldehyde-3-phosphate dehydrogenase; and to a microorganism which produces and/or secretes a fine chemical, the microorganism including the isolated polynucleotide having promoter activity, which enables various genes to be overexpressed in comparison with the particular starting strain; and to a process for preparing fine chemicals using the microorganism.