公开(公告)号：US20120117865A1

公开(公告)日：2012-05-17

申请号：US13186126

申请日：2011-07-19

申请人： Kevin L. Deppermann ,  Michael W. Petersen ,  Allen N. Ondes ,  David W. Finley ,  William M. Fischer ,  John M. Jensen ,  David Butruille ,  Stanton Dotson ,  Sam Eathington ,  Heather M. Forbes ,  Bruce Schnicker ,  John Tamulonis

发明人： Kevin L. Deppermann ,  Michael W. Petersen ,  Allen N. Ondes ,  David W. Finley ,  William M. Fischer ,  John M. Jensen ,  David Butruille ,  Stanton Dotson ,  Sam Eathington ,  Heather M. Forbes ,  Bruce Schnicker ,  John Tamulonis

IPC分类号： A01C1/00

CPC分类号： G01N35/00584 ,  A01C1/00 ,  A01C1/025 ,  G01N1/04 ,  G01N1/20 ,  G01N1/286 ,  G01N33/0098 ,  G01N33/5097 ,  G01N2001/002

摘要： A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.

摘要翻译： 提供种子采样系统，其具有包括种子仓的自动种子加载组件，并且可操作以从种子仓内的多个种子中分离种子。 该系统还包括可操作以从单个种子中去除组织样本的自动种子取样组件，以及可操作以将单种化种子从种子装载组件转移到种子采样组件的自动种子运输组件。 种子运输组件包括多个保持构件。 每个保持构件可相对于种子装载组件和种子取样组件移动。 种子运输组件可操作以将多个保持构件中的一个定位成与种子装载组件相邻以接合单个种子之一，同时将另一个保持构件定位成与种子取样组件相邻，以将另一个单个种子呈现到 种子采样总成。

公开(公告)号：US20080263721A9

公开(公告)日：2008-10-23

申请号：US11595983

申请日：2006-11-13

申请人： Andrey Boukharov ,  Yongwei Cao ,  Stanton Dotson ,  Jeffrey Koshi ,  David Kovalic ,  Jingdong Liu ,  James McIninch ,  Wei Wu

发明人： Andrey Boukharov ,  Yongwei Cao ,  Stanton Dotson ,  Jeffrey Koshi ,  David Kovalic ,  Jingdong Liu ,  James McIninch ,  Wei Wu

IPC分类号： A01H5/00 ,  C07H21/04

CPC分类号： C12N15/8216 ,  C07K14/415 ,  C12N15/8227

摘要： The present invention discloses rice genomic promoter sequences. The promoters are particularly suited for use in rice and other cereal crops. Methods of modifying, producing, and using the promoters are also disclosed. The invention further discloses compositions, transformed host cells, transgenic plants, and seeds containing the rice genomic promoter sequences, and methods for preparing and using the same.

公开(公告)号：US20050022266A1

公开(公告)日：2005-01-27

申请号：US10678588

申请日：2003-10-02

申请人： Jingrui Wu ,  Garrett Lee ,  Thomas Adams ,  Zhidong Xie ,  Stanton Dotson ,  Donald Nelson

发明人： Jingrui Wu ,  Garrett Lee ,  Thomas Adams ,  Zhidong Xie ,  Stanton Dotson ,  Donald Nelson

IPC分类号： C12N15/82 ,  A01H1/00

CPC分类号： C12N15/8273

摘要： Disclosed herein are transgenic plants having a recombinant DNA construct which expresses a Hap3 transcription factor which provides enhanced resistance and/or tolerance to water deficit. More specifically the DNA constructs comprise a polynucleotide which encodes at least a functional part of a transcription factor that is a CCAAT-box DNA binding subunit or a homologous transcription factor.

摘要翻译： 本文公开了具有表达Hap3转录因子的重组DNA构建体的转基因植物，其提供增强的抗性和/或耐水亏性。 更具体地，DNA构建体包含编码作为CCAAT盒DNA结合亚基或同源转录因子的转录因子的至少功能部分的多核苷酸。

公开(公告)号：US08312672B2

公开(公告)日：2012-11-20

申请号：US13108762

申请日：2011-05-16

申请人： Kevin L. Deppermann ,  Michael W. Petersen ,  David Butruille ,  Stanton Dotson ,  Sam Eathington ,  Heather Forbes ,  Bruce Schnicker ,  John Tamulonis

发明人： Kevin L. Deppermann ,  Michael W. Petersen ,  David Butruille ,  Stanton Dotson ,  Sam Eathington ,  Heather Forbes ,  Bruce Schnicker ,  John Tamulonis

IPC分类号： A01B79/00 ,  A01B79/02 ,  A01C1/00 ,  A01G1/00 ,  A01H3/00

CPC分类号： A01H1/02 ,  A01H1/04 ,  A01H1/08 ,  A01H5/10 ,  C12N15/8241 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156

摘要： The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for analyzing a population of haploid seeds generally includes providing a population of seeds comprising haploid seeds, removing tissue samples from a plurality of the seeds in the population using an automated seed sampler system while preserving the germination viability of the sampled seeds, and analyzing the tissue samples for the presence or absence of one or more characteristics indicative of at least one genetic or chemical trait.

摘要翻译： 本发明提供了通过使用高通量，种子的非破坏性采样来促进种质改良活动的新方法。 用于分析单倍体种子群体的方法通常包括提供包含单倍体种子的种子群体，使用自动种子取样器系统从群体中的多个种子中去除组织样本，同时保持取样种子的发芽活力，并分析 所述组织样品存在或不存在指示至少一种遗传或化学性状的一种或多种特征。

公开(公告)号：US20090136938A1

公开(公告)日：2009-05-28

申请号：US12135564

申请日：2008-06-09

申请人： Nengbing Tao ,  Zoe McCuddin ,  Stanton Dotson ,  Fenggao Dong ,  Frederic Achard ,  Sam Eathington

发明人： Nengbing Tao ,  Zoe McCuddin ,  Stanton Dotson ,  Fenggao Dong ,  Frederic Achard ,  Sam Eathington

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6895 ,  A01H1/04 ,  C12Q1/6827 ,  C12Q1/6874 ,  C12Q2600/13 ,  C12Q2600/156 ,  G16B30/00 ,  G16B35/00 ,  G16C20/60

摘要： The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants.

摘要翻译： 本发明提供了通过使用直接核酸序列信息来增强植物种质的育种方法和组合物。 该方法描述了植物繁殖群体种质中优选核酸序列的鉴定和积累。

公开(公告)号：US20070169228A1

公开(公告)日：2007-07-19

申请号：US11717562

申请日：2007-03-13

申请人： Steve He ,  Stanton Dotson

发明人： Steve He ,  Stanton Dotson

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04

CPC分类号： C07K14/415 ,  C12N15/8261 ,  C12N15/8289 ,  C12N15/829 ,  Y02A40/146

摘要： The present invention provides a gene encoding ANT-like polypeptide comprising in the N-terminal to C-terminal direction two AP2 DNA binding domains followed in the C-terminal by an amino acid subsequence selected from the group consisting of Xaa-Ser-Ser-Ser-Arg-Glu, Xaa-Ser-Asn-Ser-Arg-Glu, and Asn-Ser-Ser-Ser-Arg-Asn, wherein Xaa is an amino acid residue selected from the group consisting of Gly, Ala, Val, Leu, and Ile. Such gene encoding ANT-like polypeptide can be over-expressed in a transgenic plant to provide agronomically desired traits based on increased size of selected plant organs.

公开(公告)号：US20070020621A1

公开(公告)日：2007-01-25

申请号：US09815264

申请日：2001-03-23

申请人： Andrey Boukharov ,  Yongwei Cao ,  Stanton Dotson ,  Jeffrey Koshi ,  David Kovalic ,  Jingdong Liu ,  James McIninch ,  Wei Wu

发明人： Andrey Boukharov ,  Yongwei Cao ,  Stanton Dotson ,  Jeffrey Koshi ,  David Kovalic ,  Jingdong Liu ,  James McIninch ,  Wei Wu

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N9/00

CPC分类号： C12N15/8216 ,  C07K14/415 ,  C12N15/8227

摘要： The present invention discloses rice genomic promoter sequences. The promoters are particularly suited for use in rice and other cereal crops. Methods of modifying, producing, and using the promoters are also disclosed. The invention further discloses compositions, transformed host cells, transgenic plants, and seeds containing the rice genomic promoter sequences, and methods for preparing and using the same.

摘要翻译： 本发明公开了水稻基因组启动子序列。 促进剂特别适用于水稻和其他谷类作物。 还公开了改性，制备和使用促进剂的方法。 本发明还公开了组合物，转化的宿主细胞，转基因植物和含有水稻基因组启动子序列的种子，以及其制备和使用方法。

公开(公告)号：US20060127889A1

公开(公告)日：2006-06-15

申请号：US09912968

申请日：2001-07-25

申请人： Stanton Dotson ,  Jon Schmuke ,  John Anderson

发明人： Stanton Dotson ,  Jon Schmuke ,  John Anderson

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6851 ,  C12Q2545/101 ,  C12Q2521/107

摘要： The invention relates to the field of genetic engineering. More particularly the invention relates to a method of quantitating transgenes or transgene expression by using sequences commonly included in transformation plasmids or vectors. The invention also provides primers and probes which can be used with this method.

摘要翻译： 本发明涉及基因工程领域。 更具体地，本发明涉及通过使用通常包含在转化质粒或载体中的序列来定量转基因或转基因表达的方法。 本发明还提供可与该方法一起使用的引物和探针。

公开(公告)号：US09003696B2

公开(公告)日：2015-04-14

申请号：US13186126

申请日：2011-07-19

申请人： Kevin L. Deppermann ,  Michael W. Petersen ,  Allen N. Ondes ,  David W. Finley ,  William M. Fischer ,  John M. Jensen ,  David Butruille ,  Stanton Dotson ,  Sam Eathington ,  Heather M. Forbes ,  Bruce Schnicker ,  John Tamulonis

发明人： Kevin L. Deppermann ,  Michael W. Petersen ,  Allen N. Ondes ,  David W. Finley ,  William M. Fischer ,  John M. Jensen ,  David Butruille ,  Stanton Dotson ,  Sam Eathington ,  Heather M. Forbes ,  Bruce Schnicker ,  John Tamulonis

IPC分类号： A01C1/00 ,  G01N1/04 ,  G01N1/20 ,  G01N1/28

CPC分类号： G01N35/00584 ,  A01C1/00 ,  A01C1/025 ,  G01N1/04 ,  G01N1/20 ,  G01N1/286 ,  G01N33/0098 ,  G01N33/5097 ,  G01N2001/002

摘要： A seed sampling system is provided having an automated seed loading assembly including a seed bin and being operable to singulate seeds from a plurality of seeds within the seed bin. The system also includes an automated seed sampling assembly operable to remove tissue samples from the singulated seeds, and an automated seed transport assembly operable to transfer the singulated seeds from the seed loading assembly to the seed sampling assembly. The seed transport assembly includes multiple retention members. Each of the retention members is movable relative to the seed loading assembly and to the seed sampling assembly. The seed transport assembly is operable to position one of the multiple retention members adjacent to the seed loading assembly for engaging one of the singulated seeds, while positioning another of the retention members adjacent to the seed sampling assembly for presenting another of the singulated seeds to the seed sampling assembly.

摘要翻译： 提供种子采样系统，其具有包括种子仓的自动种子加载组件，并且可操作以从种子仓内的多个种子中分离种子。 该系统还包括可操作以从单个种子中去除组织样本的自动种子取样组件，以及可操作以将单种化种子从种子装载组件转移到种子采样组件的自动种子运输组件。 种子运输组件包括多个保持构件。 每个保持构件可相对于种子装载组件和种子取样组件移动。 种子运输组件可操作以将多个保持构件中的一个定位成邻近种子装载组件以接合单个种子中的一个，同时将另一个保持构件定位成与种子取样组件相邻，以将另一个单个种子呈现到 种子采样总成。

公开(公告)号：US20130123113A1

公开(公告)日：2013-05-16

申请号：US13247528

申请日：2011-09-28

申请人： Nengbing Tao ,  Zoe McCuddin ,  Stanton Dotson ,  Fenggao Dong ,  Frederic Achard ,  Sam Eathington

发明人： Nengbing Tao ,  Zoe McCuddin ,  Stanton Dotson ,  Fenggao Dong ,  Frederic Achard ,  Sam Eathington

IPC分类号： C12Q1/68

摘要： The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants.