公开(公告)号：US20130224870A1

公开(公告)日：2013-08-29

申请号：US13819668

申请日：2011-08-26

申请人： Gyula Vigh ,  Roy T. Estrada, III

发明人： Gyula Vigh ,  Roy T. Estrada, III

IPC分类号： C08F20/26 ,  G01N33/58 ,  G01N33/68 ,  C09B57/00

CPC分类号： C08F20/26 ,  C07C311/39 ,  C07C2603/50 ,  C07D317/22 ,  C09B57/001 ,  G01N33/54306 ,  G01N33/54393 ,  G01N33/582 ,  G01N33/6809 ,  Y10T436/173845

摘要： Solid phase fluorescent labeling reagents (SPR) are described that simultaneously capture and label analytes, and then efficiently release the labeled-analytes under mild conditions (SCaLER).

摘要翻译： 描述了固相荧光标记试剂（SPR），同时捕获和标记分析物，然后在温和条件（SCaLER）下有效释放标记分析物。

公开(公告)号：US09896522B2

公开(公告)日：2018-02-20

申请号：US13819668

申请日：2011-08-26

申请人： Gyula Vigh ,  Roy T. Estrada, III

发明人： Gyula Vigh ,  Roy T. Estrada, III

IPC分类号： G01N33/58 ,  C07C311/39 ,  C08F20/26 ,  C07D317/22 ,  G01N33/543 ,  C09B57/00 ,  G01N33/68

CPC分类号： C08F20/26 ,  C07C311/39 ,  C07C2603/50 ,  C07D317/22 ,  C09B57/001 ,  G01N33/54306 ,  G01N33/54393 ,  G01N33/582 ,  G01N33/6809 ,  Y10T436/173845

摘要： A solid phase reagent and method for simultaneously capturing and fluorescently labeling an analyte with multiple reactive sites to provide a mono-labeled analyte are disclosed. The reagent can be used in the method and comprises a plurality of analyte-reactive groups tethered to a porous solid phase, wherein each analyte-reactive group is covalently attached to a fluorescent group either directly or indirectly, wherein the distance between adjacent analyte-reactive groups is greater than the gyration radius of a captured analyte; the fluorescent group is covalently attached to a cleavable anchor group either directly or through a first spacer; and the cleavable anchor group is covalently attached to the solid phase either directly or through a second spacer, wherein the solid phase is a porous solid or a porous gel.