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公开(公告)号：US07709696B2
公开(公告)日：2010-05-04
申请号：US10887636
申请日：2004-07-09
申请人： Roger Craig , Charalambos Savakis , Frank Grosveld
发明人： Roger Craig , Charalambos Savakis , Frank Grosveld
IPC分类号： C12N15/00 , A01K67/027
CPC分类号： A01K67/0275 , A01K67/0333 , A01K67/0339 , A01K2217/05 , A01K2227/105 , A01K2267/0393 , C12N9/22 , C12N15/85 , C12N15/8509 , C12N15/90 , C12N2799/022 , C12N2799/026 , C12N2799/027 , C12N2799/028 , C12N2800/30 , C12N2800/60 , C12N2800/90 , C12N2830/003 , C12N2830/008 , C12N2830/42 , C12N2830/46 , C12N2840/203
摘要： A method of inducing transposition in a transgenic embryo, sperm and egg is described, comprising the steps of (a) generating a first adult transgenic organism comprising within its genome one or more copies of a transposon; (b) generating a second adult transgenic organism comprising within its genome one or more copies of a gene encoding a transposase cognate for the transposon and/or a sequence capable of regulating expression of the gene encoding the transposase; (c) crossing the first adult transgenic organism with the second transgenic adult organism to provide a progeny which comprises, in the genome of one or more of its cells, both (i) one or more copies of the transposon and (ii) a gene encoding a transposase cognate for the transposon, wherein the gene encoding the transposase is under the control of one or more inducible regulatory sequences which permit expression of the transposase, and (d) expressing the gene encoding the transposase in the embryo, sperm or egg to cause mobilization of the transposon within a portion of the tissues or cells of the progeny. Using the method, mobilization of a transposon can advantageously be induced at predetermined stages of development of an embryo, sperm or egg and the mutated gene of a single cell may be replicated in subsequent cell divisions, resulting in groups of cells which are essentially homogenous for the transposed gene.
摘要翻译： 描述了在转基因胚胎，精子和卵中诱导转座的方法，其包括以下步骤：（a）产生第一成体转基因生物体，其在其基因组中包含转座子的一个或多个拷贝; （b）产生第二成体转基因生物体，其在其基因组中包含编码与转座子相关的转座酶的基因的一个或多个拷贝和/或能够调节编码转座酶基因表达的序列; （c）将第一成体转基因生物与第二转基因成体生物体交叉以提供后代，其在一个或多个细胞的基因组中包含（i）转座子的一个或多个拷贝和（ii）基因 编码转座子的转座酶，其中编码转座酶的基因处于允许转座酶表达的一种或多种可诱导调节序列的控制下，和（d）在胚胎，精子或卵中表达编码转座酶的基因， 导致转座子在子代的组织或细胞的一部分内的动员。 使用该方法，可以有利地在胚胎，精子或卵的发育的预定阶段诱导转座子的动员，并且可以在随后的细胞分裂中复制单细胞的突变基因，导致基本上均匀的细胞群 转座基因。
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公开(公告)号：US07132586B2
公开(公告)日：2006-11-07
申请号：US10124903
申请日：2002-04-18
申请人： Roger Craig , Charalambos Savakis
发明人： Roger Craig , Charalambos Savakis
IPC分类号： C12P21/00 , A01K67/033
CPC分类号： C12P21/02 , A01K67/0333 , A01K67/0339 , A01K2217/05 , C07K14/47 , C07K14/61 , C12N9/22 , C12N9/2408 , C12N15/90 , C12Y302/0102
摘要： The invention relates to a method for producing a protein of interest comprising transforming a target insect with a non-viral expression system that expresses the protein in the insect larvae, breeding the insect to produce larvae, culturing the larvae and isolating the protein from the larvae.
摘要翻译： 本发明涉及一种生产目的蛋白质的方法，包括用表达昆虫幼虫蛋白质的非病毒表达系统转化靶昆虫，培育昆虫以产生幼虫，培养幼虫并从幼虫中分离蛋白质 。
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公开(公告)号：US07060506B2
公开(公告)日：2006-06-13
申请号：US09770102
申请日：2001-01-25
申请人： Roger Craig
发明人： Roger Craig
IPC分类号： G01N33/543
CPC分类号： G01N33/573
摘要： The invention provides methods and compositions for monitoring enzymatic activity as a function of the interaction of modification dependant binding partner polypeptides. Association or dissociation of the binding partner polypeptides is dependant upon the addition or removal of a moiety to or from one or both of the binding partner polypeptides or upon proteolytic digestion of one or both of the binding partner polypeptides by a modifying enzyme.
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公开(公告)号：US20200171682A1
公开(公告)日：2020-06-04
申请号：US16696736
申请日：2019-11-26
IPC分类号： B26B5/00
摘要： An ambulatory cutting device is provided. The ambulatory cutting device provides a blade mounted, by way of a blade holding assembly, to a wheeled frame. The wheeled frame has handles for a user to push the wheeled frame along a surface supporting a roofing membrane or other cuttable membrane, wherein the blade holding assembly maintains the mounted blade at an elevation just below the supporting surface so that the membrane is cut as a user pushes the wheeled frame.
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公开(公告)号：US06779130B2
公开(公告)日：2004-08-17
申请号：US09951372
申请日：2001-09-13
IPC分类号： G06F1100
CPC分类号： G06F11/1435
摘要： According to the present invention, there is provided a method, system and program storage device for maintaining an active root filesystem in a storage subsystem comprising a plurality of storage devices. The method, system and program storage device comprise searching root partitions of the plurality of storage devices for a valid root filesystem, the root filesystem including mount points associated with the plurality of storage devices; mounting a root filesystem that is found first as the active root filesystem and mounting root partitions of the plurality of storage devices to the associated mount points within the active root filesystem; and replicating the active root filesystem across the plurality of storage devices in the storage subsystem, wherein the storage subsystem is bootable from any storage device of the plurality of storage devices that includes a replica of the active root filesystem. The method, system and program storage device further replicate the active root filesystem in response to storage subsystem configuration requests, and notifications of new or replaced disk drives being installed in the storage subsystem.
摘要翻译： 根据本发明，提供了一种用于在包括多个存储设备的存储子系统中维护活动根文件系统的方法，系统和程序存储装置。 所述方法，系统和程序存储设备包括搜索所述多个存储设备中的根分区以获得有效的根文件系统，所述根文件系统包括与所述多个存储设备相关联的安装点; 将根文件系统首先安装为活动根文件系统，并将多个存储设备的根分区安装到活动根文件系统内的相关联的安装点; 以及跨所述存储子系统中的所述多个存储设备复制所述活动根文件系统，其中所述存储子系统可从包括所述活动根文件系统的副本的所述多个存储设备中的任何存储设备引导。 方法，系统和程序存储设备还响应于存储子系统配置请求复制活动根文件系统，以及安装在存储子系统中的新的或替换的磁盘驱动器的通知。
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公开(公告)号：US20060060513A1
公开(公告)日：2006-03-23
申请号：US11105030
申请日：2005-04-13
申请人： Roger Craig
发明人： Roger Craig
CPC分类号： E04H4/1263
摘要： A surface pool skimmer includes a body, a propulsion member attached to the body for propelling the body along a water surface, a skimmer attached to the body for collecting debris from the water surface, a remote control transmitter structured to transmit control signals, and a remote control receiver attached to the body and structured to receive the control signals for controlling operation of the propulsion member. A method of cleaning a pool of water includes providing an untethered surface pool skimmer having a propulsion member for propelling the surface pool skimmer along the surface of the water, and controlling the propulsion member via remote control.
摘要翻译： 表面池撇渣器包括主体，附接到主体以沿水面推进身体的推进构件，附接到身体以从水面收集碎屑的撇渣器，被构造成传送控制信号的遥控发射器，以及 遥控接收器附接到主体并被构造成接收用于控制推进构件的操作的控制信号。 一种清洁水池的方法包括提供一种无粘结表面池撇渣器，其具有用于沿水面推动表面池撇渣器的推进构件，以及通过远程控制来控制推进构件。
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公开(公告)号：US06812204B1
公开(公告)日：2004-11-02
申请号：US09748063
申请日：2000-12-22
IPC分类号： A61K3100
CPC分类号： A61K9/5068 , A61K9/0009 , A61K41/00 , A61K41/0033 , A61K47/6901
摘要： The invention relates to a method for selectively releasing an agent loaded into a red blood cell, comprising electrosensitizing the red blood cell by application of an electric field and subsequently disrupting the cell selectively using ultrasound.
摘要翻译： 本发明涉及一种用于选择性释放加载到红细胞中的药剂的方法，包括通过施加电场对红细胞进行电致敏，随后使用超声波选择性地破坏细胞。
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公开(公告)号：US20050066376A1
公开(公告)日：2005-03-24
申请号：US10887636
申请日：2004-07-09
申请人： Roger Craig , Charalambos Savakis , Frank Grosveld
发明人： Roger Craig , Charalambos Savakis , Frank Grosveld
IPC分类号： A01K67/00 , A01K67/027 , A01K67/033 , C12N9/22 , C12N15/09 , C12N15/85 , C12N15/90 , C12Q1/68
CPC分类号： A01K67/0275 , A01K67/0333 , A01K67/0339 , A01K2217/05 , A01K2227/105 , A01K2267/0393 , C12N9/22 , C12N15/85 , C12N15/8509 , C12N15/90 , C12N2799/022 , C12N2799/026 , C12N2799/027 , C12N2799/028 , C12N2800/30 , C12N2800/60 , C12N2800/90 , C12N2830/003 , C12N2830/008 , C12N2830/42 , C12N2830/46 , C12N2840/203
摘要： A method of inducing transposition in a transgenic embryo, sperm and egg is described, comprising the steps of (a) generating a first adult transgenic organism comprising within its genome one or more copies of a transposon; (b) generating a second adult transgenic organism comprising within its genome one or more copies of a gene encoding a transposase cognate for the transposon and/or a sequence capable of regulating expression of the gene encoding the transposase; (c) crossing the first adult transgenic organism with the second transgenic adult organism to provide a progeny which comprises, in the genome of one or more of its cells, both (i) one or more copies of the transposon and (ii) a gene encoding a transposase cognate for the transposon, wherein the gene encoding the transposase is under the control of one or more inducible regulatory sequences which permit expression of the transposase, and (d) expressing the gene encoding the transposase in the embryo, sperm or egg to cause mobilisation of the transposon within a portion of the tissues or cells of the progeny. Using the method, mobilisation of a transposon can advantageously be induced at predetermined stages of development of an embryo, sperm or egg and the mutated gene of a single cell may be replicated in subsequent cell divisions, resulting in groups of cells which are essentially homogenous for the transposed gene.
摘要翻译： 描述了在转基因胚胎，精子和卵中诱导转座的方法，其包括以下步骤：（a）产生第一成体转基因生物体，其在其基因组中包含转座子的一个或多个拷贝; （b）产生第二成体转基因生物体，其在其基因组中包含编码与转座子相关的转座酶的基因的一个或多个拷贝和/或能够调节编码转座酶基因表达的序列; （c）将第一成体转基因生物与第二转基因成体生物体交叉以提供后代，其在一个或多个细胞的基因组中包含（i）转座子的一个或多个拷贝和（ii）基因 编码转座子的转座酶，其中编码转座酶的基因处于允许转座酶表达的一种或多种可诱导调节序列的控制下，和（d）在胚胎，精子或卵中表达编码转座酶的基因， 导致转座子在子代的组织或细胞的一部分内的动员。 使用该方法，可以有利地在胚胎，精子或卵的发育的预定阶段诱导转座子的动员，并且可以在随后的细胞分裂中复制单细胞的突变基因，导致基本上均匀的细胞群 转座基因。
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公开(公告)号：US10994431B2
公开(公告)日：2021-05-04
申请号：US16696736
申请日：2019-11-26
摘要： An ambulatory cutting device is provided. The ambulatory cutting device provides a blade mounted, by way of a blade holding assembly, to a wheeled frame. The wheeled frame has handles for a user to push the wheeled frame along a surface supporting a roofing membrane or other cuttable membrane, wherein the blade holding assembly maintains the mounted blade at an elevation just below the supporting surface so that the membrane is cut as a user pushes the wheeled frame.
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