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    Methods and vector constructs for making non-human animals which ubiquitously express a heterologous gene
    3.
    发明授权
    Methods and vector constructs for making non-human animals which ubiquitously express a heterologous gene 有权
    用于制造普遍表达异源基因的非人动物的方法和载体构建体

    公开(公告)号：US06461864B1

    公开(公告)日：2002-10-08

    申请号：US09291541

    申请日：1999-04-14

    申请人： Philippe Soriano ,  Elizabeth J. Robertson

    发明人： Philippe Soriano ,  Elizabeth J. Robertson

    IPC分类号： C12N1563

    CPC分类号： A01K67/0275 ,  A01K2217/05 ,  A01K2217/07 ,  A01K2217/15 ,  A01K2217/20 ,  A01K2217/30 ,  C12N9/00 ,  C12N15/87

    摘要： Transgenic non-human animals one having a general deletor construct and a second having a general reporter construct are described. The general deletor animals express a heterologous recombinase under the control of an ubiquitously expressed endogenous promoter. Specifically, the Cre recombinase is inserted into the ROSA26 locus of the mouse. The general reporter animals have a gene which is desired to remove flanked by sites recognized by the is heterologous recombinase. This flanked sequence is operatively associated with a marker gene such that when the gene sequence flanked by sites recognized by the heterologous recombinase is excised, the reporter gene is expressed. When the general deletor mouse is crossed with the general reporter mouse the heterologous recombinase is expressed in essentially all cells of the resultant descendants under the control of the ubiquitous promoter. Expression of the recombinase results in the excision of the desired gene in essentially all cells of the descendant animals. A conditional deletor construct and its use to derive a conditional deletor animal are also described.

    摘要翻译： 描述了具有一般缺失构建体的转基因非人动物和具有一般报告基因构建体的第二非动物。 一般的删除动物在普遍表达的内源性启动子的控制下表达异源重组酶。 具体来说，将Cre重组酶插入小鼠的ROSA26位点。 一般报道动物具有希望被异源重组酶所识别的位点侧向除去的基因。 该侧翼序列可操作地与标记基因相关联，使得当切除由异源重组酶识别的位点侧翼的基因序列时，报告基因被表达。 当一般的缺失小鼠与一般报道小鼠杂交时，异源重组酶在无处不在的启动子控制下在所得后代的基本上所有细胞中表达。 重组酶的表达导致在后代动物的基本上所有细胞中切除所需基因。 还描述了条件删除构造及其用于导出条件删除动物的用途。