Restoration of Nucleic Acid From Degraded or Formalin-Fixed and Paraffin-Embedded Tissue and Uses Thereof
    1.
    发明申请
    Restoration of Nucleic Acid From Degraded or Formalin-Fixed and Paraffin-Embedded Tissue and Uses Thereof 有权
    从降解或福尔马林固定和石蜡包埋组织中恢复核酸及其用途

    公开(公告)号:US20120009570A1

    公开(公告)日:2012-01-12

    申请号:US12087951

    申请日:2007-02-26

    申请人: Olivier Loudig

    发明人: Olivier Loudig

    摘要: This invention provides methods, primers and kits for restoration of nucleic acid from tissue, in particular degraded tissue and formalin-fixed and paraffin-embedded (FFPE) tissue, where the methods involve complementary-template reverse-transcription (CT-RT) where short single-stranded DNA sequences reverse-transcribed from mRNA are used for reverse-transcription of complementary sense-RNA templates. The methods can be used to determine patterns of gene expression and chromosomal alterations in archived tissue samples, and may be used to identify expression of disease-related genes.

    摘要翻译: 本发明提供用于从组织,特别是降解组织和福尔马林固定和石蜡包埋(FFPE)组织中恢复核酸的方法,引物和试剂盒,其中所述方法涉及互补模板逆转录(CT-RT),其中短 用mRNA反向转录的单链DNA序列用于互补有义RNA模板的逆转录。 该方法可用于确定归档组织样品中基因表达和染色体变化的模式,并可用于鉴定疾病相关基因的表达。

    Restoration of nucleic acid from degraded or formalin-fixed and paraffin-embedded tissue and uses thereof
    2.
    发明授权
    Restoration of nucleic acid from degraded or formalin-fixed and paraffin-embedded tissue and uses thereof 有权
    从降解或福尔马林固定和石蜡包埋组织中恢复核酸及其用途

    公开(公告)号:US08497067B2

    公开(公告)日:2013-07-30

    申请号:US12087951

    申请日:2007-02-26

    申请人: Olivier Loudig

    发明人: Olivier Loudig

    IPC分类号: C12P19/34

    摘要: This invention provides methods, primers and kits for restoration of nucleic acid from tissue, in particular degraded tissue and formalin-fixed and paraffin-embedded (FFPE) tissue, where the methods involve complementary-template reverse-transcription (CT-RT) where short single-stranded DNA sequences reverse-transcribed from mRNA are used for reverse-transcription of complementary sense-RNA templates. The methods can be used to determine patterns of gene expression and chromosomal alterations in archived tissue samples, and may be used to identify expression of disease-related genes.

    摘要翻译: 本发明提供用于从组织,特别是降解组织和福尔马林固定和石蜡包埋(FFPE)组织中恢复核酸的方法,引物和试剂盒,其中所述方法涉及互补模板逆转录(CT-RT),其中短 用mRNA反向转录的单链DNA序列用于互补有义RNA模板的逆转录。 该方法可用于确定归档组织样品中基因表达和染色体变化的模式,并可用于鉴定疾病相关基因的表达。