公开(公告)号：US20240192202A1

公开(公告)日：2024-06-13

申请号：US18533104

申请日：2023-12-07

Applicant: Nautilus Subsidiary, Inc.

Inventor： Rukshan PERERA ,  Maryam JOUZI ,  Cassandra STAWICKI ,  Torri Elise RINKER ,  Chol YUN ,  Kenneth KUHN ,  Raymond MAK

IPC: G01N33/543 ,  G01N33/58

CPC classification number: G01N33/54306 ,  G01N33/582

Abstract: Methods of minimizing detection of unintended signals during array-based processes are provided. Methods include the use of sets of fluidic media that inhibit sources of unintended signals during array-based processes. Also provided are systems containing the sets of fluidic media that are configured to perform the array-based processes provided herein.

公开(公告)号：US20240158782A1

公开(公告)日：2024-05-16

申请号：US18402273

申请日：2024-01-02

Applicant: Nautilus Subsidiary, Inc.

Inventor： Parag MALLICK

IPC: C12N15/10 ,  G01N33/68

CPC classification number: C12N15/1055 ,  G01N33/6845

Abstract: Methods and systems are provided herein for selecting an affinity reagent which binds a desired peptide epitope in a plurality of sequence contexts. The method relies on obtaining a peptide library, each peptide having the sequence αXβ, wherein X is the desired peptide epitope, wherein each of α and β comprise an amino acid, using the peptide library to select an affinity reagent.

公开(公告)号：US20240053333A1

公开(公告)日：2024-02-15

申请号：US18364200

申请日：2023-08-02

Applicant: Nautilus Subsidiary, Inc.

Inventor： Seok Ki CHOI ,  Julia ROBINSON

IPC: G01N33/533 ,  C07K19/00 ,  C07K1/08 ,  C07K1/13

CPC classification number: G01N33/533 ,  C07K19/00 ,  C07K1/084 ,  C07K1/13

Abstract: Provided are antibody conjugates, including a pair of cysteines crosslinked via a two-carbon bridge covalently connecting the sulfur atoms of the cysteines. Methods for making and using antibody conjugates are also provided. The compositions and methods of the present disclosure although particularly well suited to antibodies can be extended to other analytes having sulfur moieties such as proteins obtained from biological or artificial sources.

公开(公告)号：US20230314324A1

公开(公告)日：2023-10-05

申请号：US18192606

申请日：2023-03-29

Applicant: Nautilus Subsidiary, Inc.

Inventor： Maryam JOUZI ,  Torri Elise RINKER ,  Pengyu HAO ,  Vadim LOBANOV ,  Ryan Kempston SEGHERS ,  Daniel HORNER ,  Pierre INDERMUHLE ,  David STERN ,  Ezra VAN GELDER ,  Kevin CHEN

IPC: G01N21/64 ,  B82Y15/00

CPC classification number: G01N21/6452 ,  G01N21/6428 ,  B82Y15/00 ,  G01N2021/6439

Abstract: Compositions, systems, and methods are disclosed for preparing and utilizing arrays, such as single-analyte arrays containing a plurality of fiducial elements with random spatial distributions. Arrays may be prepared with pluralities of fiducial elements comprising optically active or passive moieties. Arrays containing random spatial distributions of fiducial elements may be utilized for various array-based processes that require spatial information.

公开(公告)号：US20230287480A1

公开(公告)日：2023-09-14

申请号：US18180733

申请日：2023-03-08

Applicant: Nautilus Subsidiary, Inc.

Inventor： David STERN ,  Glenn CHAN ,  Ezra VAN GELDER

IPC: C12Q1/6837 ,  C12Q1/6825 ,  C12Q1/6876

CPC classification number: C12Q1/6837 ,  C12Q1/6825 ,  C12Q1/6876 ,  C12Q2600/158

Abstract: Systems for optically detecting single-analytes, such as cells, nucleic acids, and polypeptides, are described. The described optical detection systems are suitable for multiplexed detection of single-analytes, including single-analytes provided in an array-based format. Methods for identifying single-analyte properties and interactions utilizing optical detection systems are provided.

公开(公告)号：US20230272459A1

公开(公告)日：2023-08-31

申请号：US18179924

申请日：2023-03-07

Applicant: Nautilus Subsidiary, Inc.

Inventor： Tural AKSEL ,  Torri RINKER ,  Markus BURNS ,  Michael DORWART ,  Rachel GALIMIDI ,  Dmitriy GREMYACHINSKIY ,  Stephen HENDRICKS ,  Elvis IKWA ,  Gregory KAPP ,  Joshua Simon KLEIN ,  Julia ROBINSON ,  Cassandra STAWICKI ,  Sonal TONAPI ,  Parag MALLICK

IPC: C12Q1/6832 ,  C12Q1/6834 ,  C12Q1/6876

CPC classification number: C12Q1/6832 ,  C12Q1/6834 ,  C12Q1/6876

Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.

公开(公告)号：US11721412B2

公开(公告)日：2023-08-08

申请号：US16534174

申请日：2019-08-07

Applicant: NAUTILUS SUBSIDIARY, INC.

Inventor： Sujal M. Patel ,  Parag Mallick

IPC: G16B20/20

CPC classification number: G16B20/20

Abstract: Methods and systems are provided for accurate and efficient identification and quantification of proteins. In an aspect, disclosed herein is a method for identifying a protein in a sample of unknown proteins, comprising receiving information of a plurality of empirical measurements performed on the unknown proteins; comparing the information of empirical measurements against a database comprising a plurality of protein sequences, each protein sequence corresponding to a candidate protein among a plurality of candidate proteins; and for each of one or more of the plurality of candidate proteins, generating a probability that the candidate protein generates the information of empirical measurements, a probability that the plurality of empirical measurements is not observed given that the candidate protein is present in the sample, or a probability that the candidate protein is present in the sample; based on the comparison of the information of empirical measurements against the database.

公开(公告)号：US11692217B2

公开(公告)日：2023-07-04

申请号：US17523869

申请日：2021-11-10

Applicant: Nautilus Subsidiary, Inc.

Inventor： Tural Aksel ,  Torri Rinker ,  Markus Burns ,  Michael Dorwart ,  Rachel Galimidi ,  Dmitriy Gremyachinskiy ,  Stephen Hendricks ,  Elvis Ikwa ,  Gregory Kapp ,  Joshua Simon Klein ,  Julia Robinson ,  Cassandra Stawicki ,  Sonal Tonapi ,  Parag Mallick

IPC: C12Q1/6832 ,  C12Q1/6834 ,  C12Q1/6876

CPC classification number: C12Q1/6832 ,  C12Q1/6834 ,  C12Q1/6876

Abstract: An affinity reagent, having: (a) a retaining component such as a structured nucleic acid particle; and (b) one or both of (i) one or more label components attached to the retaining component, and (ii) one or more binding components attached to the retaining component.

公开(公告)号：US20250066841A1

公开(公告)日：2025-02-27

申请号：US18813886

申请日：2024-08-23

Applicant: Nautilus Subsidiary, Inc.

Inventor： Robert GROTHE ,  Kara JUNEAU ,  Michael Augusto DARCY ,  Parag MALLICK ,  Jacinto VILLANUEVA ,  Vivekananda BUDAMAGUNTA ,  Jonathan LEANO ,  Pengyu HAO ,  Terren CHANG ,  Aimee SANFORD ,  Maureen NEWMAN ,  Filip BARTNICKI ,  Rukshan PERERA ,  Grant NAPIER

IPC: C12Q1/6818 ,  G01N33/542

Abstract: Provided are methods of detecting analytes. In some configurations, the methods can employ analytes attached to a solid support or particle and affinity reagents that are attached to the solid support or particle via a flexible linker. In some configurations, the methods can employ analytes attached to a solid support or particle and solution-phase affinity reagents can be attracted to the analytes via application of a stimulus.

公开(公告)号：US20240368585A1

公开(公告)日：2024-11-07

申请号：US18770462

申请日：2024-07-11

Applicant: Nautilus Subsidiary, Inc.

Inventor： Pengyu HAO ,  Elvis IKWA ,  Pierre INDERMUHLE ,  Christina INMAN ,  Parag MALLICK ,  Torri Elise RINKER ,  Steven TAN ,  Tural AKSEL ,  Stephen HENDRICKS ,  Sadie INGLE

IPC: C12N15/10 ,  B01J19/00 ,  B82Y5/00 ,  C12Q1/6804 ,  C12Q1/6837

Abstract: Compositions, systems, and methods for the display of analytes such as biomolecules are described. Display of analytes is achieved by coupling of the analytes to displaying molecules that are configured to associate with surfaces or interfaces. Arrays of analytes may be formed from the described systems for utilization in assays and other methods.