公开(公告)号：US20230092310A1

公开(公告)日：2023-03-23

申请号：US17799323

申请日：2021-02-15

Applicant: Nuclera Nucleics Ltd.

Inventor： Michael Chun Hao Chen ,  Sihong Chen ,  Christopher Dulson ,  Sumit Kalsi ,  Gordon Ross McInroy ,  Stephanie Reikine

IPC: B01L3/00

Abstract: Disclosed is a method for the cell-free expression of peptides or proteins in a liquid filled digital microfluidic device. The droplets having the components required for cell-free protein expression can be manipulated by electrokinesis in order to enhance levels of protein expression in the droplets.

公开(公告)号：US20230087259A1

公开(公告)日：2023-03-23

申请号：US17903120

申请日：2022-09-06

Applicant: Nuclera Nucleics Ltd.

Inventor： Michael CHEN ,  Jiahao HUANG ,  Radu LAZAR ,  Gordon MCINROY

IPC: C12P19/34 ,  C07H1/02 ,  C07H19/10 ,  C07H19/20 ,  C40B50/04 ,  C40B50/18 ,  C40B80/00

Abstract: The invention relates to methods of synthesizing polymers, biopolymers, and nucleic acids, to immobilised dNTP/NTPs and kits comprising said immobilised dNTP/NTPs for use in said methods of nucleic acid synthesis.

公开(公告)号：US20220127588A1

公开(公告)日：2022-04-28

申请号：US17407509

申请日：2021-08-20

Applicant: Nuclera Nucleics Ltd.

Inventor： Michael Chun Hao Chen ,  Sihong Chen ,  Gordon Ross McInroy

IPC: C12N9/12

Abstract: The invention relates to engineered terminal deoxynucleotidyl transferase (TdT) enzymes or the homologous amino acid sequence of Polμ, Polβ, Polλ, and Polθ of any species or the homologous amino acid sequence of X family polymerases of any species and uses thereof.

公开(公告)号：US20220111387A1

公开(公告)日：2022-04-14

申请号：US17497512

申请日：2021-10-08

Applicant: Nuclera Nucleics Ltd.

Inventor： Richard J. Paolini, JR. ,  Luke M. Slominski ,  Timothy J. O'Malley ,  David Zhitomirsky

IPC: B01L3/00

Abstract: An electrowetting system for actuating droplets of a first composition and of a second composition. The system includes: a plurality of electrodes configured to manipulate droplets of fluid in a microfluidic space, each electrode being coupled to circuitry which applies driving voltages to the electrode; and a processing unit operably connected to a look up table correlating drive sequences to chemical species and at least one composition parameter. The processing unit is configured to: receive data of a first chemical species and a first composition parameter of the first composition; receive data of a second chemical species and a second composition parameter of the second composition; correlate a first drive sequence with the first chemical species and the first composition parameter; correlate a second drive sequence with the second chemical species and the second composition parameter; and output the first drive sequence and the second drive sequence to the electrodes.

公开(公告)号：US11236377B2

公开(公告)日：2022-02-01

申请号：US16923656

申请日：2020-07-08

Applicant: Nuclera Nucleics Ltd.

Inventor： Michael C. Chen ,  Radu A. Lazar ,  Jiahao Huang ,  Gordon R. McInroy

IPC: C12P19/34 ,  C12N9/12 ,  C12Q1/68

Abstract: The invention relates to the use of specific terminal deoxynucleotidyl transferase (TdT) enzymes in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of terminal deoxynucleotidyl transferases and 3′-blocked nucleotide triphosphates in a method of template independent nucleic acid synthesis.

公开(公告)号：US20210261998A1

公开(公告)日：2021-08-26

申请号：US17176686

申请日：2021-02-16

Applicant: Nuclera Nucleics Ltd.

Inventor： Michael C. Chen ,  Radu A. Lazar ,  Jiahao Huang ,  Gordon R. McInroy

IPC: C12P19/34 ,  C07H1/00 ,  C07H21/00 ,  C07H19/10 ,  C07H19/12 ,  C07H19/20

Abstract: The invention relates to a method of nucleic acid synthesis comprising the use of 3′-O-azidomethyl blocked nucleotide triphosphates which comprises the step of adding a capping group to any uncleaved 3′-O-azidomethyl groups and to the use of kits comprising said capping groups in a method of nucleic acid synthesis. The invention also relates to capped nucleotide triphosphates and 3′-O-azidomethyl capping groups.

公开(公告)号：US10745727B2

公开(公告)日：2020-08-18

申请号：US15549602

申请日：2016-02-10

Applicant: Nuclera Nucleics Ltd.

Inventor： Michael C. Chen ,  Radu A. Lazar ,  Jiahao Huang ,  Gordon R. McInroy

IPC: C12P19/34 ,  C12N9/12 ,  C12Q1/68

Abstract: The invention relates to the use of specific terminal deoxynucleotidyl transferase (TdT) enzymes in a method of nucleic acid synthesis, to methods of synthesizing nucleic acids, and to the use of kits comprising said enzymes in a method of nucleic acid synthesis. The invention also relates to the use of terminal deoxynucleotidyl transferases and 3′-blocked nucleotide triphosphates in a method of template independent nucleic acid synthesis.

公开(公告)号：US20190338331A1

公开(公告)日：2019-11-07

申请号：US16480128

申请日：2018-01-26

Applicant: Nuclera Nucleics Ltd.

Inventor： Michael Chen ,  Jiahao Huang ,  Radu Lazar ,  Gordon McInroy

IPC: C12P19/34 ,  C07H19/10 ,  C07H19/20 ,  C07H1/02 ,  C40B80/00 ,  C40B50/04 ,  C40B50/18

Abstract: The invention relates to methods of synthesizing polymers, biopolymers, and nucleic acids, to immobilised dNTP/NTPs and kits comprising said immobilised dNTP/NTPs for use in said methods of nucleic acid synthesis.

公开(公告)号：US20230175030A1

公开(公告)日：2023-06-08

申请号：US17610900

申请日：2020-05-14

Applicant: Nuclera Nucleics Ltd.

Inventor： Michael Chun Hao Chen ,  Gordon Ross McInroy ,  Martin Edward Fox ,  Michal Robert Matuszewski

IPC: C12P19/34 ,  C12P19/18 ,  C07H21/04

CPC classification number: C12P19/34 ,  C12P19/18 ,  C07H21/04

Abstract: Disclosed an improved process for synthesising a nucleic acid strand using cycles of template independent enzyme extension. Having one or more of the amino groups on the base heterocyclic groups masked with protecting groups helps to prevent secondary structure in the extended strand, thereby improving access of the enzyme to the 3′ OH terminus for extension.

公开(公告)号：US11623940B2

公开(公告)日：2023-04-11

申请号：US16646361

申请日：2018-09-14

Applicant: Nuclera Nucleics Ltd.

Inventor： Michael Chen ,  Jiahao Huang ,  Gordon McInroy

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6806 ,  C12P19/34

Abstract: The invention relates to the use of polyphosphate containing species in a method of nucleic acid synthesis, to methods of nucleic acid synthesis, and to the use of kits comprising said polyphosphate containing species. The invention also relates to the use of polyphosphate containing species for the capping of 3′-terminal hydroxyl moieties using terminal transferases.