公开(公告)号：US5541076A

公开(公告)日：1996-07-30

申请号：US460496

申请日：1995-06-02

申请人： Raymond L. Houghton ,  Morgan Van Aken ,  Tobin K. Jones

发明人： Raymond L. Houghton ,  Morgan Van Aken ,  Tobin K. Jones

IPC分类号： C07K16/30 ,  G01N33/543 ,  G01N33/574 ,  G01N33/576 ,  G01N33/68 ,  G01N33/53

CPC分类号： C07K16/3038 ,  G01N33/54313 ,  G01N33/57415 ,  G01N33/57423 ,  G01N33/5743 ,  G01N33/57438 ,  G01N33/57488 ,  G01N33/576 ,  G01N33/6887 ,  G01N2800/34 ,  Y10S436/813

摘要： Complexes (which include basement membrane components) and their polypeptide components are found to be related to the invasiveness of a bladder tumor. Detection of such complexes or constituents in a urine sample by immunological or non-immunological methods permits determining the invasiveness of a bladder tumor.

摘要翻译： 发现复合物（其包括基底膜组分）及其多肽组分与膀胱肿瘤的侵袭性有关。 通过免疫学或非免疫学方法检测尿样中的这种复合物或成分允许确定膀胱肿瘤的侵袭性。

公开(公告)号：US5512657A

公开(公告)日：1996-04-30

申请号：US178219

申请日：1994-01-06

申请人： Morgan Van Aken ,  Stefan L. Paskell

发明人： Morgan Van Aken ,  Stefan L. Paskell

IPC分类号： C07K16/30 ,  G01N33/543 ,  G01N33/574 ,  G01N33/576 ,  G01N33/68 ,  C07K14/435 ,  C07K1/22 ,  G01N33/483 ,  G01N33/493

CPC分类号： C07K16/3038 ,  G01N33/54313 ,  G01N33/57415 ,  G01N33/57423 ,  G01N33/5743 ,  G01N33/57438 ,  G01N33/57488 ,  G01N33/576 ,  G01N33/6887 ,  G01N2800/34 ,  Y10S436/811 ,  Y10S436/813 ,  Y10S436/82

摘要： Isolated complexes (which include basement membrane components), antibodies to such complexes or polypeptide constituents thereof, and methods for detecting such complexes or constituents are disclosed. Detection of such complexes in a biological sample by immunological and non-immunological methods allows the diagnosis of a variety of diseases, including cancers, collagen degenerative diseases, and hepatitis. Suitable biological samples include urine, cervical secretions, bronchial aspirates, sputum, saliva, feces, serum, synovial fluid, and cerebrospinal fluid.

摘要翻译： 公开了分离的复合物（其包括基底膜组分），这种复合物或其多肽组分的抗体，以及用于检测这些复合物或组分的方法。 通过免疫学和非免疫学方法检测生物样品中的这种复合物允许诊断各种疾病，包括癌症，胶原退行性疾病和肝炎。 合适的生物样品包括尿液，宫颈分泌物，支气管抽吸物，痰液，唾液，粪便，血清，滑液和脑脊液。

公开(公告)号：US5591595A

公开(公告)日：1997-01-07

申请号：US457285

申请日：1995-06-01

申请人： Morgan Van Aken ,  Stefan L. Paskell

发明人： Morgan Van Aken ,  Stefan L. Paskell

IPC分类号： C07K16/30 ,  G01N33/543 ,  G01N33/574 ,  G01N33/576 ,  G01N33/68 ,  G01N33/53

CPC分类号： C07K16/3038 ,  G01N33/54313 ,  G01N33/57415 ,  G01N33/57423 ,  G01N33/5743 ,  G01N33/57438 ,  G01N33/57488 ,  G01N33/576 ,  G01N33/6887 ,  G01N2800/34 ,  Y10S436/811 ,  Y10S436/813 ,  Y10S436/82

摘要： Isolated complexes (which include basement membrane components), antibodies to such complexes or polypeptide constituents thereof, and methods for detecting such complexes or constituents are disclosed. Detection of such complexes in a biological sample by immunological and non-immunological methods allows the diagnosis of a variety of diseases, including cancers, collagen degenerative diseases, and hepatitis. Suitable biological samples include urine, cervical secretions, bronchial aspirates, sputum, saliva, feces, serum, synovial fluid, and cerebrospinal fluid.

摘要翻译： 公开了分离的复合物（其包括基底膜组分），这种复合物或其多肽组分的抗体，以及用于检测这些复合物或组分的方法。 通过免疫学和非免疫学方法检测生物样品中的这种复合物允许诊断各种疾病，包括癌症，胶原退行性疾病和肝炎。 合适的生物样品包括尿液，宫颈分泌物，支气管抽吸物，痰液，唾液，粪便，血清，滑液和脑脊液。

公开(公告)号：US5489537A

公开(公告)日：1996-02-06

申请号：US23906

申请日：1993-02-23

申请人： Morgan Van Aken

发明人： Morgan Van Aken

IPC分类号： G01N33/543 ,  G01N33/574 ,  G01N33/58 ,  G01N33/546 ,  G01N33/547 ,  G01N33/558

CPC分类号： G01N33/54313 ,  G01N33/57438 ,  G01N33/585 ,  Y10S435/97 ,  Y10S435/975

摘要： The present invention provides methods and kits for determining the presence or amount of a substance by detection of a colloidal dye associated with agglutinated particles. The disclosure of the present invention shows that the use of a suspension of colloidal dye, which contains dye unattached to the particles to be agglutinated, enhances the amount of colloidal dye associated with the particles following agglutination. The methods and kits are disclosed in direct and indirect (e.g., competitive) formats. In one aspect, the present invention provides methods and kits utilizing a single colloidal dye. In another aspect, methods and kits are provided which include two colloidal dyes, wherein one colloidal dye functions as a background-enhancing dye. A related aspect of the present invention provides methods and kits that include a colloidal dye and a non-water-soluble dye (which functions as a background-enhancing dye) attached to a microporous pad which permits differential migration of non-agglutinated and agglutinated particles. In yet another aspect, the present invention provides methods and kits utilizing a colloidal dye and a water-soluble dye, which functions as a background-enhancing dye.

摘要翻译： 本发明提供用于通过检测与凝集颗粒相关的胶体染料来确定物质的存在或量的方法和试剂盒。 本发明的公开内容表明，使用含有未附着于要凝集的颗粒的染料的胶体染料的悬浮液增加了凝集后与颗粒相关的胶态染料的量。 方法和试剂盒以直接和间接（例如，竞争性）的形式公开。 一方面，本发明提供了使用单一胶态染料的方法和试剂盒。 在另一方面，提供了包括两种胶体染料的方法和试剂盒，其中一种胶体染料用作背景增强染料。 本发明的相关方面提供了包括胶态染料和附着于微孔垫的非水溶性染料（其起到背景增强染料的作用）的方法和试剂盒，其允许非凝集和凝集的颗粒的差异迁移 。 在另一方面，本发明提供了利用胶体染料和作为背景增强染料的水溶性染料的方法和试剂盒。

公开(公告)号：US5591830A

公开(公告)日：1997-01-07

申请号：US456855

申请日：1995-06-01

申请人： Morgan Van Aken ,  Stefan L. Paskell

发明人： Morgan Van Aken ,  Stefan L. Paskell

IPC分类号： C07K16/30 ,  G01N33/543 ,  G01N33/574 ,  G01N33/576 ,  G01N33/68 ,  C07K16/00 ,  C07K16/18

CPC分类号： C07K16/3038 ,  G01N33/54313 ,  G01N33/57415 ,  G01N33/57423 ,  G01N33/5743 ,  G01N33/57438 ,  G01N33/57488 ,  G01N33/576 ,  G01N33/6887 ,  G01N2800/34 ,  Y10S436/811 ,  Y10S436/813 ,  Y10S436/82

摘要： Isolated complexes (which include basement membrane components), antibodies to such complexes or polypeptide constituents thereof, and methods for detecting such complexes or constituents are disclosed. Detection of such complexes in a biological sample by immunological and non-immunological methods allows the diagnosis of a variety of diseases, including cancers, collagen degenerative diseases, and hepatitis. Suitable biological samples include urine, cervical secretions, bronchial aspirates, sputum, saliva, feces, serum, synovial fluid, and cerebrospinal fluid.