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    Modulation of Immunity to Drug Resistant and Latent MTB
    3.
    发明申请
    Modulation of Immunity to Drug Resistant and Latent MTB 有权

    公开(公告)号：US20220088166A1

    公开(公告)日：2022-03-24

    申请号：US17022893

    申请日：2020-09-16

    申请人： Longhorn Vaccines and Diagnostics, LLC

    发明人： Gerald W. Fischer ,  Luke T. Daum ,  Richard F. Schuman ,  Clara J. Sei

    IPC分类号： A61K39/04 ,  A61K45/06 ,  A61P31/06

    摘要： The invention is directed to compositions and methods for stimulating, enhancing or modulating the immune system of a patient before or after infection by a pathogen, and in particular multidrug resistant (MDR) MTB and extremely drug resistant (XDR) MTB. Compositions of the invention contain non-naturally occurring antigens that generate an effective cellular and/or humoral immune response to MTB and/or antibodies that are specifically reactive to MTB antigens. The greater activity of the immune system generated by a vaccine of the invention increases generation of memory T cells that provide for a greater and/or extended response to an MTB infection. Responses involve an increased generation of antibodies that enhance immunity against MTB infection and promote an enhanced phagocytic response. Monoclonal antibodies produced by the non-naturally occurring antigens enhance phagocytosis and killing of mycobacteria by phagocytic cells, enhance clearance of MTB from the blood and modulate immunity and cytokine responses.

    Next generation genomic sequencing methods
    4.
    发明授权
    Next generation genomic sequencing methods 有权

    公开(公告)号：US11186867B2

    公开(公告)日：2021-11-30

    申请号：US15448966

    申请日：2017-03-03

    申请人： Longhorn Vaccines and Diagnostics, LLC

    发明人： Luke T. Daum ,  Gerald W. Fischer

    IPC分类号： C12Q1/68 ,  C12Q1/6869 ,  C12Q1/689 ,  C12Q1/70

    摘要： Disclosed is an enhanced method for rapid and cost-effective analysis of sequences of a microorganism by semi-conductor sequencing, preferably ion-torrent sequencing. This method provides for full length analysis and of multiple areas (e.g. genes) of multiple genomes. These methods identify genetic mutations of a particular gene that are responsible for conferring resistance or sensitivity to an antibiotic or other chemical compound. Multiple different species, strains and/or serotypes of a particular organism are rapidly and efficiently screened and mutations identified along with the complete genome of an organism. By selecting primers pairs of similar size and GC content that produce amplicons with sequences spanning the entire genome, a single PCR reaction analyzed by ion torrent methodology can determine the sequence of a complete genome. Methods are useful to sequences the genomes of viral agents, such as influenza virus, and bacterial agents, such as tuberculosis bacteria.

    Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
    5.
    发明授权
    Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples 有权

    公开(公告)号：US11041216B2

    公开(公告)日：2021-06-22

    申请号：US15361228

    申请日：2016-11-25

    申请人： Longhorn Vaccines and Diagnostics, LLC

    发明人： Jeffrey D. Fischer ,  Luke T. Daum ,  Gerald W. Fischer

    IPC分类号： C12Q1/68 ,  C12N15/10 ,  C12Q1/70 ,  C12Q1/689 ,  C12Q1/6806

    摘要： The invention is directed to compositions and methods for rapidly detecting, amplifying, and quantitating one or more pathogen-specific nucleic acids in a biological sample, and in particular, samples obtained from patients with sepsis. The invention also provides diagnostic kits containing specific amplification primers, and labeled detection probes that specifically bind to the amplification products obtained therefrom. The invention is also directed to detecting the quantity or ratio of genomic sequences and mRNA sequences of an individual suspected of being infected with an infectious agent over time to assess the progress of the infection over time. Also disclosed are compositions and methods for the isolation and characterization of nucleic acids that are specific to one or more pathogens, such as, for example, Influenza virus, Mycobacterium tuberculosis, Plasmodium, and/or HIV from a wide variety of samples including those of biological, environmental, clinical and/or veterinary origin.

    Antibodies that modulate immunity to drug resistant and latent MTB infections
    6.
    发明授权
    Antibodies that modulate immunity to drug resistant and latent MTB infections 有权

    公开(公告)号：US10370437B2

    公开(公告)日：2019-08-06

    申请号：US15850208

    申请日：2017-12-21

    申请人： Longhorn Vaccines and Diagnostics, LLC

    发明人： Gerald W. Fischer ,  Luke T. Daum ,  Richard F. Schuman ,  Clara J. Sei

    IPC分类号： A61K39/02 ,  C07K16/12 ,  A61K45/06 ,  A61K39/40 ,  A61K39/04 ,  A61P31/06 ,  A61K39/00

    摘要： The invention is directed to compositions and methods for stimulating, enhancing or modulating the immune system of a patient before or after infection by a pathogen, and in particular multidrug resistant (MDR) MTB and extremely drug resistant (XDR) MTB. Compositions of the invention contain non-naturally occurring antigens that generate an effective cellular and/or humoral immune response to MTB and/or antibodies that are specifically reactive to MTB antigens. The greater activity of the immune system generated by a vaccine of the invention increases generation of memory T cells that provide for a greater and/or extended response to an MTB infection. Responses involve an increased generation of antibodies that enhance immunity against MTB infection and promote an enhanced phagocytic response. Monoclonal antibodies produced by the non-naturally occurring antigens enhance phagocytosis and killing of mycobacteria by phagocytic cells, enhance clearance of MTB from the blood and modulate immunity and cytokine responses.

    PCR Ready Compositions and Methods for Detecting and Identifying Nucleic Acid Sequences
    10.
    发明申请
    PCR Ready Compositions and Methods for Detecting and Identifying Nucleic Acid Sequences 审中-公开
    PCR准备组合物和检测和鉴定核酸序列的方法

    公开(公告)号：US20170044595A1

    公开(公告)日：2017-02-16

    申请号：US15334519

    申请日：2016-10-26

    申请人： Longhorn Vaccines and Diagnostics, LLC

    发明人： Gerald W. Fischer ,  Luke T. Daum

    IPC分类号： C12Q1/68

    摘要： The invention is directed to compositions and methods for isolating, detecting, amplifying, and quantitating pathogen-specific nucleic acids in a biological sample, and in particular PCR ready compositions that contain enzyme and are stable or long periods of time. The invention also provides diagnostic kits containing specific amplification primers, and labeled detection probes that specifically bind to the amplification products obtained therefrom. Also disclosed are compositions and methods for the isolation and characterization of nucleic acids that are specific to one or more pathogens, including for example Influenza virus and Mycobacterium tuberculosis, from a wide variety of samples including those of biological, environmental, clinical and/or veterinary origin.

    摘要翻译： 本发明涉及用于分离，检测，扩增和定量生物样品中的病原体特异性核酸的组合物和方法，特别是含有酶且稳定或长时间段的PCR即用组合物。 本发明还提供了包含特异性扩增引物的诊断试剂盒，以及特异性结合从其获得的扩增产物的标记检测探针。 还公开了用于分离和表征对于一种或多种病原体（包括例如流感病毒和结核分枝杆菌）特异性的核酸的组合物和方法，所述多种样品包括生物，环境，临床和/或兽医学 起源。