公开(公告)号：US06790669B1

公开(公告)日：2004-09-14

申请号：US10018629

申请日：2001-12-14

申请人： Curtis D. Pfeiffer ,  Nile N. Frawley ,  Thomas L. Peters ,  Philip J. Savickas ,  David R. Albers ,  Steven J. Gluck ,  Lawrence W. Nicholson ,  Jose B. Esquivel H.

发明人： Curtis D. Pfeiffer ,  Nile N. Frawley ,  Thomas L. Peters ,  Philip J. Savickas ,  David R. Albers ,  Steven J. Gluck ,  Lawrence W. Nicholson ,  Jose B. Esquivel H.

IPC分类号： G01N3014

CPC分类号： G01N30/88 ,  G01N30/78 ,  G01N2030/625 ,  G01N2030/8813 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/255

摘要： A chemical analysis method for determining chemically related differences between subject biological material such as genetically modified plant material and control biological material such as genetically unmodified plant material, which method includes at least the following six steps. The first step is to contact the subject biological material with a fluid extractant, such as a mixture of water, isopropanol and potassium hydroxide, to produce a fluid extract of the subject biological material. The second step is to contact the control biological material with the fluid extractant to produce a fluid extract of the control biological material. The third step is to chromatograph the fluid extract of the subject biological material, for example, gas or fluid chromatography, to produce a chromatogram of the fluid extract of the subject biological material. The fourth step is to chromatograph the fluid extract of the control biological material to produce a chromatogram of the fluid extract of the control biological material. The fifth step is to determine the differences between the chromatograms, for example, by using the method of U.S. Pat. No. 5,592,402, to identify at least one outlier peak. The sixth step is to determine the chemical identity of the outlier peak, for example, using gas chromatography/mass spectroscopy analysis of the outlier peak.

摘要翻译： 一种化学分析方法，用于确定受试生物材料如转基因植物材料和对照生物材料如基因未修饰植物材料之间的化学相关差异，该方法至少包括以下六个步骤。 第一步是用流体萃取剂如水，异丙醇和氢氧化钾的混合物接触主题生物材料，以产生受试者生物材料的流体提取物。 第二步是将控制生物材料与流体萃取剂接触以产生对照生物材料的流体萃取物。 第三步是对目标生物材料的流体提取物进行色谱分离，例如气相色谱或流体色谱法，以产生该物质生物材料的液体提取物的色谱图。 第四步是对对照生物材料的流体提取物进行色谱分析以产生对照生物材料的液体提取物的色谱图。 第五步是确定色谱图之间的差异，例如，使用美国专利No. 确定至少一个异常值峰值的第5,592,402号。 第六步是确定离子峰的化学特性，例如使用离子峰的气相色谱/质谱分析。

公开(公告)号：US5641404A

公开(公告)日：1997-06-24

申请号：US596338

申请日：1996-02-20

申请人： Lawrence W. Nicholson ,  Christian T. Goralski ,  Curtis D. Pfeiffer

发明人： Lawrence W. Nicholson ,  Christian T. Goralski ,  Curtis D. Pfeiffer

IPC分类号： B01D15/08 ,  C07B57/00

CPC分类号： B01D15/3833 ,  B01D15/203 ,  C07B57/00

摘要： A process for separating enantiomeric mixtures by liquid chromatography using a stationary phase that includes cellulose or amylose derivative and a mobile phase that includes methanol and pentane, the concentration of methanol in the mobile phase being greater than one tenth percent by volume and less than the saturation concentration of methanol in the mobile phase, the concentration of pentane being at least that necessary to resolve the enantiomeric mixture into its enantiomers with a resolution at least one and one half times greater than the pentane of the mobile phase is replaced with hexane.

摘要翻译： PCT No.PCT / US94 / 09687 371日期1996年2月20日 102（e）日期1996年2月20日PCT 1994年8月25日PCT公布。 第WO95 / 05879号公报 日期1995年3月2日使用包含纤维素或直链淀粉衍生物的固定相和包含甲醇和戊烷的流动相通过液相色谱分离对映异构体混合物的方法，流动相中甲醇的浓度大于体积百分之十 并且小于流动相中甲醇的饱和浓度，所替代的戊烷浓度至少为将对映异构体混合物分解成其对映异构体所必需的浓度至少比流动相的戊烷高1.5倍。 用己烷。

公开(公告)号：US5367073A

公开(公告)日：1994-11-22

申请号：US138613

申请日：1993-10-15

申请人： Bakthan Singaram ,  Gary B. Fisher ,  Christian T. Goralski ,  Lawrence W. Nicholson

发明人： Bakthan Singaram ,  Gary B. Fisher ,  Christian T. Goralski ,  Lawrence W. Nicholson

IPC分类号： C07C213/00 ,  C07C215/08 ,  C07D295/084 ,  C07D295/088 ,  C07D295/096 ,  C07D207/12 ,  C07D295/08 ,  C07C213/38

CPC分类号： C07D295/096 ,  C07C213/00 ,  C07C215/08 ,  C07D295/084 ,  C07D295/088 ,  C07D295/092

摘要： The present invention relates to a process for the synthesis of chiral enantiomerically pure .beta.-amino alcohols which are extraordinarily important as therapeutic agents for the treatment of a variety of human disorders and as chiral auxiliaries in organic synthesis. The hydroboration of enamines is a versatile and convenient method for the synthesis of both racemic and enantiomerically pure .beta.-amino alcohols in high yields. This methodology enables the synthesis of virtually any .beta.-amino alcohol. Hydroboration of these enamines with chiral organic borohydrides, e.g. either mono- or diisopinocampheylborane, followed by oxidation with aqueous or solid NaOH/30% H.sub.2 O.sub.2 or Me.sub.3 NO, gives the corresponding chiral .beta.-amino alcohol. Enantiomeric excesses ranged from 60% for reactions run at 25.degree. C. to greater than 99% for reactions run at -25.degree. C.

摘要翻译： 本发明涉及合成手性对映异构纯的β-氨基醇的方法，其作为用于治疗多种人类疾病的治疗剂非常重要，并且作为有机合成中的手性助剂。 烯胺的硼氢化合物是以高产率合成外消旋和对映异构纯的β-氨基醇的通用和方便的方法。 该方法使得几乎可以合成任何β-氨基醇。 这些烯胺与手性有机硼氢化物的水解，例如 单或二蒎烷基硼烷，然后用水或固体NaOH / 30％H 2 O 2或Me 3 NO 3氧化，得到相应的手性β-氨基醇。 在25℃下进行的反应，对映异构体的过量范围为60％，对于在-25℃下进行的反应为大于99％

公开(公告)号：US4837161A

公开(公告)日：1989-06-06

申请号：US900038

申请日：1986-08-25

申请人： Timothy S. Stevens ,  Nile N. Frawley ,  Daniel J. Swart ,  William C. Harris ,  Deborah E. Diedering ,  Lawrence W. Nicholson ,  L. David Rothman

发明人： Timothy S. Stevens ,  Nile N. Frawley ,  Daniel J. Swart ,  William C. Harris ,  Deborah E. Diedering ,  Lawrence W. Nicholson ,  L. David Rothman

IPC分类号： G01N1/38 ,  G01N30/02 ,  G01N30/84 ,  G01N35/08

CPC分类号： G01N35/085 ,  G01N30/84 ,  G01N2001/381 ,  G01N2030/8435 ,  G01N30/02 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/255

摘要： An analytical chemistry apparatus and method for introducing a reagent into a flowing stream of liquid carrier in order to quantitatively analyze one or more components of a sample added into the carrier. The reagent is permeated across a short section of membrane having relatively large pores. The use of such a membrane: (a) allows the membrane to be protected from physical damage by covering it with a perforate structure; (b) significantly reduces bandspreading across the membrane reagent addition device; (c) reduces the pressure drop across the membrane reagent addition device; and (d) still allows for the permeation of an effective amount of the reagent into the carrier. The reagent is preferably pressurized to minimize leakage of carrier across the membrane and the reagent can be self-pressurized by essentially completely filling the reagent reservoir of the invention with reagent and then hermetically sealing the reservoir.

摘要翻译： 一种分析化学装置和方法，用于将试剂引入液体载体的流动流中，以便定量分析添加到载体中的样品的一种或多种组分。 试剂渗入具有较大孔隙的短截面膜。 使用这种膜：（a）允许通过用穿孔结构覆盖膜来保护膜免受物理损伤; （b）显着减少穿过膜试剂添加装置的带宽; （c）降低膜试剂添加装置上的压降; 和（d）仍然允许有效量的试剂渗透到载体中。 试剂优选被加压以最小化载体穿过膜的泄漏，并且试剂可以通过用试剂基本上完全填充本发明的试剂储存器然后密封储存器而自加压。