公开(公告)号：US5756313A

公开(公告)日：1998-05-26

申请号：US138384

申请日：1993-10-20

申请人： Ken Okabayashi ,  Hideyuki Oi ,  Kazumoto Hirabayashi ,  Masami Miura ,  Miho Shimizu ,  Haruhide Kawabe

发明人： Ken Okabayashi ,  Hideyuki Oi ,  Kazumoto Hirabayashi ,  Masami Miura ,  Miho Shimizu ,  Haruhide Kawabe

IPC分类号： C12N1/19 ,  C07K14/765 ,  C12N9/26 ,  C12N15/09 ,  C12N15/14 ,  C12N15/81 ,  C12N15/90 ,  C12P21/00 ,  C12P21/02 ,  C12R1/865 ,  C12N15/00

CPC分类号： C07K14/765 ,  C12N15/81 ,  C12N15/90 ,  C12N9/2408 ,  C07K2319/02

摘要： A plasmid for a yeast host, the plasmid comprising in sequence: (1) a yeast derived promoter, (2) an albumin-encoding region placed under control of the yeast-derived promoter, (3) a transcription terminator and (4) a sequence homologous to a part of the yeast host chromosomal sequence such that the plasmid is capable of being integrated into the yeast host cell chromosome and wherein the plasmid is incapable of autonomous replication in yeast cells; a yeast host transformed with the above plasmid; a method of producing a yeast transformant which comprises transforming a yeast host by integrating at least two different plasmids each having a sequence homologous to a part of the host yeast chromosomal sequence one at a time into the chromosome of the yeast host cell; and a method of producing albumin which comprises cultivating the above transformant and recovering the thus-produced albumin.

摘要翻译： 一种用于酵母宿主的质粒，所述质粒按顺序包含：（1）酵母衍生的启动子，（2）置于酵母衍生的启动子控制下的白蛋白编码区，（3）转录终止子和（4） 序列与酵母宿主染色体序列的一部分同源，使得质粒能够整合到酵母宿主细胞染色体中，并且其中质粒不能在酵母细胞中自主复制; 用上述质粒转化的酵母宿主; 生产酵母转化体的方法，其包括通过将至少两种与宿主酵母染色体部分的序列同源的不同质粒整合到酵母宿主细胞的染色体中来转化酵母宿主; 以及生产白蛋白的方法，其包括培养上述转化体并回收由此产生的白蛋白。

公开(公告)号：US4757021A

公开(公告)日：1988-07-12

申请号：US795144

申请日：1985-11-05

申请人： Haruhide Kawabe ,  Kazumoto Hirabayashi ,  Hazime Horii ,  Hirofumi Arimura ,  Masayuki Nishida ,  Tadakazu Suyama

发明人： Haruhide Kawabe ,  Kazumoto Hirabayashi ,  Hazime Horii ,  Hirofumi Arimura ,  Masayuki Nishida ,  Tadakazu Suyama

IPC分类号： C12N15/09 ,  C07K14/02 ,  C12N1/16 ,  C12N15/68 ,  C12N15/81 ,  C12P21/00 ,  C12R1/865 ,  C12N1/18 ,  C12N1/00 ,  C12N15/00

CPC分类号： C07K14/005 ,  C12N15/68 ,  C12N15/81 ,  C12N2730/10122

摘要： A recombinant plasmid wherein at least two DNA fragments containing a Baker gene and a Charlie gene of the yeast 2 .mu.m circular plasmid are incorporated into a plasmid containing genes encoding a physiologically active substance is disclosed. Also, a strain transformed by the recombinant plasmid is disclosed.

摘要翻译： 公开了将包含酵母2μm圆形质粒的Baker基因和Charlie基因的至少两个DNA片段并入包含编码生理活性物质的基因的质粒中的重组质粒。 此外，公开了由重组质粒转化的菌株。