公开(公告)号：US11142544B2

公开(公告)日：2021-10-12

申请号：US15307499

申请日：2015-04-28

申请人： Juewen Liu ,  Po-Jung Jimmy Huang

发明人： Juewen Liu ,  Po-Jung Jimmy Huang

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12N15/11 ,  C12N15/10

摘要： A novel substrate-bound DNAzyme complex is provided comprising a DNAzyme bound to a nucleic acid-based substrate. The DNAzyme comprises a pair of binding arms which hybridize to binding regions on the substrate, and a catalytic domain between the binding arms. The nucleic acid-based substrate comprises a phosphorothioate-modified ribonucleotide cleavage site between the binding regions of the substrate. The catalytic domain of the DNAzyme catalyzes heavy metal-dependent cleavage of the substrate cleavage site. The DNAzyme complex is useful in a method of heavy metal sensing. A novel cadmium-selective DNAzyme is also described for cadmium sensing.

公开(公告)号：US08043802B2

公开(公告)日：2011-10-25

申请号：US11926779

申请日：2007-10-29

申请人： Yi Lu ,  Juewen Liu

发明人： Yi Lu ,  Juewen Liu

IPC分类号： C12Q1/68 ,  C12M1/34 ,  G01N33/00 ,  G01N33/53 ,  C07H21/04

CPC分类号： C12Q1/68 ,  C12Q1/6825 ,  Y10S436/80 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/501 ,  C12Q2563/137 ,  C12Q2521/337 ,  C12Q2541/101

摘要： A novel biosensor comprises at least one fluorophore and at least two quenchers, and is capable of selectively and specifically detecting the presence of an ion in the presence of other ions.

公开(公告)号：US20110123982A1

公开(公告)日：2011-05-26

申请号：US12598070

申请日：2008-07-16

申请人： Yi Lu ,  Juewen Liu

发明人： Yi Lu ,  Juewen Liu

IPC分类号： C12Q1/68 ,  C07H21/00

CPC分类号： C12N15/115 ,  C12N2310/3517 ,  C12N2320/10 ,  G01N33/542 ,  G01N33/84

摘要： A nucleic acid enzyme responsive to copper, comprising an oligonucleotide comprising a nucleotide sequence of SEQ ID NO:1, wherein the nucleic acid enzyme is not self-cleaving.

摘要翻译： 一种对铜有响应的核酸酶，其包含含有SEQ ID NO：1的核苷酸序列的寡核苷酸，其中所述核酸酶不是自切割的。

公开(公告)号：US20100105039A1

公开(公告)日：2010-04-29

申请号：US12476756

申请日：2009-06-02

申请人： Yi Lu ,  Zidong Wang ,  Jung Heon Lee ,  Juewen Liu

发明人： Yi Lu ,  Zidong Wang ,  Jung Heon Lee ,  Juewen Liu

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6825 ,  C12Q2521/337 ,  C12Q2563/137 ,  C12Q2563/149

摘要： The present invention provides a sensor system kit for detecting an analyte, consisting essentially of: a nucleic acid enzyme, wherein the nucleic acid enzyme cleaves a substrate in the presence of the analyte; the substrate for the nucleic acid enzyme, comprising a polynucleotide; an aggregator; and particles.

摘要翻译： 本发明提供了用于检测分析物的传感器系统试剂盒，其基本上由以下组成：核酸酶，其中所述核酸酶在分析物存在下切割底物; 用于核酸酶的底物，包含多核苷酸; 聚合器 和颗粒。

公开(公告)号：US20090029874A1

公开(公告)日：2009-01-29

申请号：US12206871

申请日：2008-09-09

申请人： Yi Lu ,  Juewen Liu

发明人： Yi Lu ,  Juewen Liu

IPC分类号： C40B30/08 ,  C12M1/00 ,  C12Q1/68

CPC分类号： C12Q1/68 ,  C12Q1/6825 ,  Y10S436/80 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/501 ,  C12Q2563/137 ,  C12Q2521/337 ,  C12Q2541/101

摘要： A novel biosensor comprises at least one fluorophore and at least two quenchers, and is capable of selectively and specifically detecting the presence of an ion in the presence of other ions.

公开(公告)号：US20050282186A1

公开(公告)日：2005-12-22

申请号：US11082197

申请日：2005-03-16

申请人： Yi Lu ,  Juewen Liu

发明人： Yi Lu ,  Juewen Liu

IPC分类号： C12Q1/68

CPC分类号： C12Q1/68 ,  C12Q1/6825 ,  Y10S436/80 ,  Y10S977/924 ,  Y10T436/143333 ,  C12Q2565/501 ,  C12Q2563/137 ,  C12Q2521/337 ,  C12Q2541/101

摘要： A novel biosensor comprises at least one fluorophore and at least two quenchers, and is capable of selectively and specifically detecting the presence of an ion in the presence of other ions.

摘要翻译： 一种新型生物传感器包括至少一种荧光团和至少两种猝灭剂，并且能够在其它离子存在下选择性地和具体地检测离子的存在。

公开(公告)号：US20240261220A1

公开(公告)日：2024-08-08

申请号：US18106205

申请日：2023-02-06

申请人： JUEWEN LIU ,  YIBO LIU ,  CENTRE FOR EYE AND VISION RESEARCH LIMITED ,  THE HONG KONG POLYTECHNIC UNIVERSITY

发明人： MAN-SAU WONG ,  KA-YING WONG ,  JUEWEN LIU ,  YIBO LIU

IPC分类号： A61K9/127 ,  A61K38/13 ,  A61K47/54 ,  A61P27/02

CPC分类号： A61K9/127 ,  A61K38/13 ,  A61K47/549 ,  A61P27/02

摘要： Provided herein are aptamer functionalized liposomes for delivery of ocular drug for eye diseases (such as dry eye disease), products comprising the same and method for preventing and/or treating eye diseases using the same. The aptamer functionalized liposomes, product and method of the present application can be used to effectively delivering ocular drug with extended retention time and high protective effect.

公开(公告)号：US20170241971A1

公开(公告)日：2017-08-24

申请号：US15307499

申请日：2015-04-28

申请人： Juewen Liu ,  Po-Jung Jimmy Huang

发明人： Juewen Liu ,  Po-Jung Jimmy Huang

IPC分类号： G01N33/20 ,  C12Q1/68 ,  C12N15/11

CPC分类号： G01N33/20 ,  C07H21/04 ,  C12N15/10 ,  C12N15/111 ,  C12N2310/127 ,  C12N2310/315 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2320/10 ,  C12Q1/68

摘要： A novel substrate-bound DNAzyme complex is provided comprising a DNAzyme bound to a nucleic acid-based substrate. The DNAzyme comprises a pair of binding arms which hybridize to binding regions on the substrate, and a catalytic domain between the binding arms. The nucleic acid-based substrate comprises a phosphorothioate-modified ribonucleotide cleavage site between the binding regions of the substrate. The catalytic domain of the DNAzyme catalyzes heavy metal-dependent cleavage of the substrate cleavage site. The DNAzyme complex is useful in a method of heavy metal sensing. A novel cadmium-selective DNAzyme is also described for cadmium sensing.

公开(公告)号：US08992984B1

公开(公告)日：2015-03-31

申请号：US12909572

申请日：2010-10-21

申请人： C. Jeffrey Brinker ,  Carlee Erin Ashley ,  Xingmao Jiang ,  Juewen Liu ,  David S. Peabody ,  Walker Richard Wharton ,  Eric Carnes ,  Bryce Chackerian ,  Cheryl L. Willman

发明人： C. Jeffrey Brinker ,  Carlee Erin Ashley ,  Xingmao Jiang ,  Juewen Liu ,  David S. Peabody ,  Walker Richard Wharton ,  Eric Carnes ,  Bryce Chackerian ,  Cheryl L. Willman

IPC分类号： A61K9/14 ,  A61K9/51

CPC分类号： A61K9/127 ,  A61K9/1277 ,  A61K9/5115 ,  A61K9/5123 ,  A61K31/513 ,  A61K31/575 ,  A61K31/704 ,  A61K33/24 ,  A61K49/005

摘要： Various embodiments provide materials and methods for synthesizing protocells for use in targeted delivery of cargo components to cancer cells. In one embodiment, the lipid bilayer can be fused to the porous particle core to form a protocell. The lipid bilayer can be modified with targeting ligands or other ligands to achieve targeted delivery of cargo components that are loaded within the protocell to a target cell, e.g., a type of cancer. Shielding materials can be conjugated to the surface of the lipid bilayer to reduce undesired non-specific binding.

摘要翻译： 各种实施方案提供用于合成原细胞以用于向癌细胞靶向递送货物组分的材料和方法。 在一个实施方案中，脂质双层可以与多孔颗粒核心融合以形成原细胞。 脂质双层可以用靶向配体或其他配体进行修饰，以实现载体在原细胞内的货物组分的靶向递送到靶细胞，例如一种类型的癌症。 屏蔽材料可以结合到脂质双层的表面以减少不期望的非特异性结合。

公开(公告)号：US20120141551A1

公开(公告)日：2012-06-07

申请号：US13310287

申请日：2011-12-02

申请人： Steven BLOEMBERGEN ,  Ian J. MCLENNAN ,  Nathan JONES ,  Ryan WAGNER ,  Aareet Krsna GANESH SHERMON ,  Abdel Rahman ELSAYED ,  Juewen LIU

发明人： Steven BLOEMBERGEN ,  Ian J. MCLENNAN ,  Nathan JONES ,  Ryan WAGNER ,  Aareet Krsna GANESH SHERMON ,  Abdel Rahman ELSAYED ,  Juewen LIU

IPC分类号： A61K9/14 ,  A61P35/00 ,  C08B31/00 ,  A61K47/36 ,  B82Y5/00

CPC分类号： A61K9/145 ,  A61K9/1676 ,  A61K9/5161 ,  A61K31/704 ,  A61K47/36 ,  A61K47/549 ,  A61K47/6939 ,  B82Y5/00 ,  C08B31/003 ,  C08B31/185 ,  C08B33/00 ,  C08B35/00 ,  Y10S977/773 ,  Y10S977/906 ,  Y10T428/2982

摘要： A delivery device for an active agent comprises nanoparticles based on a biopolymer such as starch. The delivery device may also be in the form of an aptamer-biopolymer-active agent conjugate wherein the aptamer targets the device for the treatment of specific disorders. The nanoparticles may be made by applying a high shear force in the presence of a crosslinker. The particles may be predominantly in the range of 50-150 nm and form a colloidal dispersion of crosslinked hydrogel particles in water. The biopolymer may be functionalized. The aptamer may be conjugated directly to the cross-linked biopolymers. The active agent may be a drug useful for the treatment of cancer. The delivery device survives for a period of time in the body sufficient to allow for the sustained release of a drug and for the transportation and uptake of the conjugate into targeted cells. However, the biopolymer is biocompatible and resorbable.

摘要翻译： 用于活性剂的递送装置包括基于诸如淀粉的生物聚合物的纳米颗粒。 递送装置还可以是适体 - 生物聚合物 - 活性剂缀合物的形式，其中适体靶向用于治疗特定病症的装置。 可以通过在交联剂的存在下施加高剪切力来制备纳米颗粒。 颗粒可以主要在50-150nm的范围内，并形成交联的水凝胶颗粒在水中的胶态分散体。 生物聚合物可以官能化。 适体可以直接与交联的生物聚合物缀合。 活性剂可以是用于治疗癌症的药物。 递送装置在体内存活足够长的时间以允许药物的持续释放以及将缀合物运输和摄取到靶细胞中。 然而，生物聚合物是生物相容性和可再吸收的。