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    Method of diagnosis of foot and mouth disease and the diagnostic kit
    5.
    发明申请
    Method of diagnosis of foot and mouth disease and the diagnostic kit 审中-公开
    口蹄疫诊断方法及诊断试剂盒

    公开(公告)号：US20070128587A1

    公开(公告)日：2007-06-07

    申请号：US10555059

    申请日：2003-05-06

    申请人： In-Soo Cho ,  Bang-Hun Hyun ,  Kwang-Nyeong Lee ,  Jae-Ku Oem ,  Soo-Jeong Kye ,  Young-Joon Ko ,  Bok-Kyung Ku ,  Yi-Seok Joo ,  Soo-Hwan An ,  In-Joong Kim ,  Ok-Kyung Kim ,  Hee-Jeong Kim ,  Ki-Yong Jang ,  Nam-Kyu Shin ,  Suh-Ha Hwang ,  Je-Mo Kang ,  Chang-Ho Kim ,  Song-Woo Ko

    发明人： In-Soo Cho ,  Bang-Hun Hyun ,  Kwang-Nyeong Lee ,  Jae-Ku Oem ,  Soo-Jeong Kye ,  Young-Joon Ko ,  Bok-Kyung Ku ,  Yi-Seok Joo ,  Soo-Hwan An ,  In-Joong Kim ,  Ok-Kyung Kim ,  Hee-Jeong Kim ,  Ki-Yong Jang ,  Nam-Kyu Shin ,  Suh-Ha Hwang ,  Je-Mo Kang ,  Chang-Ho Kim ,  Song-Woo Ko

    IPC分类号： C12Q1/70

    CPC分类号： G01N33/56983 ,  G01N33/526 ,  G01N33/558 ,  G01N2333/09 ,  Y10S436/811

    摘要： The present invention provides a method for diagnosing foot-and-mouth disease virus infection, comprising the steps of applying a predetermined amount of a test sample to a loading region of a strip; coupling a detection reagent including a given labeling reagent to an analyte of interest in the test sample to form a complex therebetween; developing the complex onto a wicking membrane; and observing changes in appearance of a reactivity zone having at least more than one immobilized phase selected from antigen, antibody or hapten on the predetermined region of the wicking membrane, derived from FMDV or obtainable from FMDV through an immunological reaction to determine the presence or absence of foot-and-mouth disease virus infection. It also provides a kit for diagnosing foot-and-mouth disease virus infection comprising a strip 1 including a reactivity zone 13 containing at least more than one immobilized phase selected from antigen, antibody or hapten thereon, derived from FMDV or obtainable from FMDV through an immunological reaction, and a control zone 14 for confining normal operation of the kit, provided on a predetermined region of a wicking membrane 9; and a housing 20 protecting the strip 1 from a variety of contaminants, and including at least a test sample application port 2 and an indicia window 4 for observing results of reaction in the reactivity zone 13 and the control zone 14 on the strip.

    摘要翻译： 本发明提供了一种用于诊断口蹄疫病毒感染的方法，包括以下步骤：将预定量的测试样品施加到条带的装载区域; 将包含给定标记试剂的检测试剂与测试样品中感兴趣的分析物偶联以在其间形成络合物; 将复合物发展到芯吸膜上; 并且观察到具有至少多于一个选自抗原，抗体或半抗原的固定相的反应性区域的变化，所述固定相选自FMDV的来自FMDV的预定区域，或者可通过免疫反应从FMDV获得，以确定存在或不存在 的口蹄疫病毒感染。 它还提供了用于诊断口蹄疫病毒感染的试剂盒，其包括条带1，其包含反应性区13，反应性区13含有至少多于一种固定化相，其选自抗原，抗体或半抗原，其衍生自FMDV或可从FMDV通过 免疫反应和用于限制试剂盒的正常操作的控制区14，其设置在芯吸膜9的预定区域上; 以及保护条带1免受各种污染物的壳体20，并且至少包括测试样品施加口2和标记窗口4，用于观察条带上的反应性区域13和控制区域14中的反应结果。